Vars (NM_053292) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bat6; G7a; TrsVal; Vars2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR208326 representing NM_053292 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCATCCTCTACGTCTCTCCCCACCCCGACGCCTTCCCCAGCCTGCGAGCCCTCATCGCTGCCCGCT ACGGGGAAGCTGGGGACGGTCCCGGGTGGGGAGGCCCTCACCCTCGCATCTGCCTGCAGCCTCCCCCGAG CAGCCGGACTCCATTCCCCCCGCCTCGCCTGCCCGCCCTGGAGCAGGGGCCTGGCGGCCTGTGGGTGTGG GGGGCCCCGGCTGTAGCTCAGCTGCTGTGGCCAGCAGGCCTGGGAGGACCCGGGGGCAGCCGGGCAGCTG TCCTGGTCCAGCAGTGGGTCAGTTATGCCGACACGGAGCTCACACCAGCTGCCTGCGGGGCGACGCTGCC TGCTCTAGGACTTCGAGGTCCTGGTCAGGATCCCCAGGCTGCCCTTGGGGCCCTGGGCAAGGCCTTGAAC CCCTTGGAGGAATGGCTTCGTCTGCACACCTACCTGGCTGGGGACGCTCCCACTCTGGCTGACTTAGCTG CTGTGACAGCCTTACTGCTGCCCTTCCGATACGTTCTGGACCCTTCTGCCCGCCGGATCTGGGGTAATGT GACTCGCTGGTTTAACACCTGTGTCCGGCAACCAGAATTCCGGGCTGTGCTGGGAGAAGTGGTCCTGTAC TCAGGGGCCAGGTCTGTCACTCAGCAGCCAGGTTCCGAGATCACTGCACCCCAAAAGACAGCTGCTCAGC TCAAGAAAGAAGCAAAGAAACGGGAGAAACTAGAGAAATTCCAACAGAAGCAGAAGACGCAGCAGCAGCA GCCGGCACACGGGGAGAAGAAACCAAAACCAGAGAAGAAGGAGAAGCGAGACCCCGGGGTCATTACCTAT GACCTCCCTACCCCACCTGGGGAGAAGAAAGACGTAAGTGGCACCATGCCCGACTCCTACAGCCCTCAGT ATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCGGCAGGGCTTCTTCAAGCCAGAGTACGGGCGTCCTAG CGTGTCAGCACCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAACGTGACAGGTTCCCTG CACCTGGGCCACGCGCTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCACCGCATGCGGGGGGAGA CCACCCTATGGAACCCAGGCTGTGACCATGCTGGCATCGCCACCCAGGTGGTGGTGGAAAAGAAACTCTG GAAAGAGCGGGGTCTGAACCGTCACCAGCTGGGCCGCGAGGCCTTTCTTCAGGAGGTCTGGAAGTGGAAA GCGGAGAAGGGTGACAGGATTTACCACCAATTAAAGAAACTTGGCAGCTCCTTGGACTGGGATCGAGCCT GCTTCACCATGGATCCAAAATTGTCAGCAACTGTGACAGAAGCTTTTGTTAGGCTCCATGAAGAAGGGGT CATTTACCGCAGTACCCGCCTGGTCAACTGGTCCTGCACCCTCAACTCTGCCATTTCTGACATCGAGGTG GATAAGAAAGAGCTGACGGGCCGCACCCTGCTCTCTGTGCCTGGCTACAAGGAGAAGGTGGAGTTTGGGG TCCTCGTGTCCTTTGCCTACAAGGTCCAAGGCTCAGACAGCGACGAGGAGGTGGTGGTGGCAACAACCCG GATCGAGACAATGCTGGGGGACGTGGCTGTGGCTGTGCACCCCAAAGATCCCAGATACCAGCATTTAAAG GGAAAGAGTGTCGTCCACCCGTTCTTGTCCCGGAGCCTTCCCATTGTCTTTGATGACTTTGTGGACATGG AGTTTGGCACAGGTGCTGTGAAGATCACCCCGGCCCATGACCAGAATGACTATGAGGTTGGGCAGCGACA TAGGCTGGAGGCCATTAGCATCATGGACTCGAAGGGGGCCCTTGTCAATGTGCCTCCACCTTTCCTGGGC CTGCCCAGGTTTGAGGCCAGGAAGGCTGTGCTGGCGGCCCTGAAGGAGCAGGGCCTGTTCCGCGGCATCA AGGACAACCCCATGGTAGTGCCACTATGCAACCGCTCCAAGGATGTGGTGGAGCCTCTGCTAAGGCCACA GTGGTACGTGCGCTGTGGGGAGATGGCTCAGGCCGCCAGTGCTGCCGTGACCCGGGGAGACCTCCGTATA CTGCCTGAGGCCCATCAGCGGACGTGGCATTCTTGGATGGACAACATCCGAGACTGGTGCATCTCTCGGC AGCTGTGGTGGGGCCACCGAATCCCTGCCTACTTCATCACTGTCCATGACCCAGCGGTACCCCCTGGGGA GGACCCTGATGGGCGGTACTGGGTAAGCGGACGCACTGAAGCAGAGGCCCGGGAGAAGGCAGCACGGGAA TTTGGAGTGTCCCCTGACAAGATCAGCCTTCAGCAAGATGAGGATGTGTTAGACACCTGGTTCTCGTCTG GTCTCTTTCCCTTCTCCATCTTTGGCTGGCCCAATCAGTCAGAAGATCTCAGTGTGTTCTACCCTGGGAC CCTGCTGGAGACGGGCCATGATATCCTCTTCTTCTGGGTGGCGCGAATGGTCATGCTTGGCCTGAAGCTC ACTGAGAAGCTGCCCTTCAGAGAGGTCTACCTCCATGCAATCGTGCGGGACGCTCATGGCCGGAAGATGA GCAAGTCTCTCGGCAATGTCATCGACCCCCTGGATGTCATCCATGGAGTTTCCTTGCAGGGCCTCCATGA CCAACTACTGAACAGCAACCTGGATCCCAGTGAGGTGGAGAAAGCCAAAGAAGGACAGAGGGCCGACTTT CCAGCAGGGATTCCCGAGTGTGGCACTGATGCCCTACGCTTTGGACTCTGTGCCTACACATCCCAAGGTC GAGACATTAACCTGGATGTGAACAGGATCCTGGGGTACCGTCACTTCTGCAACAAACTCTGGAATGCCAC CAAGTTTGCTTTGCGCGGCCTTGGGAAGGGCTTTGTACCCTCACCAACCTCCAAGCCGGAAGGGCACGAG AGCCTGGTGGACCGCTGGATCCGCAGCCGGCTGGCGGAGGCCGTGAGGCTCAGCAATGAAGGCTTCCAGG CTTATGATTTCCCAGCCGTCACCACTGCCCAGTACAGCTTTTGGCTCTATGAGCTCTGTGATGTCTACTT GGAGTGCCTAAAACCCGTTCTGAACGGAGTGGACCAGGTAGCAGCAGACTGTGCTCGGCAAACCCTCTAC ACCTGCCTGGATGTCGGCCTGCGGCTGCTCTCACCCTTCATGCCCTTTGTCACAGAGGAGCTATTCCAGA GGCTGCCTCGGCGGACACCAAACGCTCCTGCTAGCCTCTGTGTCACCCCCTACCCAGAGCCCTCAGAGTG CTCCTGGAAGGACCCTGAAGCCGAAGCTGCTCTTGAGCTAGCGCTGAGCATCACTAGAGCTGTGCGCTCC CTGCGTGCAGACTACAACCTGACCCGGACCAGGCCCGACTGTTTCTTGGAAGTAGCTGATGAGGCTACGG GTGCCTTGGCCTCGGCAGTGTCGGCCTACGTGCAGACTCTGGCCAGTGCGGGTGTGGTGGCCGTCCTGGC CCTGGGTGCTCCCGCACCACAGGGCTGCGCTGTGGCTGTGGCCTCAGACCGCTGCTCCATCCACCTGCAG CTGCAGGGGCTCGTGGACCCAGCCCGGGAGCTGGGCAAGCTGCAGGCCAAGCGGAGTGAGGCACAGCGAC AGGCTCAGCGGCTGCAGGAGCGCCGTGCTGCCTCTGGCTACTCAGCAAAGGTGCCCCTTGAGGTCCAGGA GGCCGACGAAGTGAAGTTACAACAGACAGAGGCGGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTGTTT CAGAAGATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR208326 representing NM_053292 Red=Cloning site Green=Tags(s) MSILYVSPHPDAFPSLRALIAARYGEAGDGPGWGGPHPRICLQPPPSSRTPFPPPRLPALEQGPGGLWVW GAPAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELTPAACGATLPALGLRGPGQDPQAALGALGKALN PLEEWLRLHTYLAGDAPTLADLAAVTALLLPFRYVLDPSARRIWGNVTRWFNTCVRQPEFRAVLGEVVLY SGARSVTQQPGSEITAPQKTAAQLKKEAKKREKLEKFQQKQKTQQQQPAHGEKKPKPEKKEKRDPGVITY DLPTPPGEKKDVSGTMPDSYSPQYVEAAWYPWWERQGFFKPEYGRPSVSAPNPRGVFMMCIPPPNVTGSL HLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWKERGLNRHQLGREAFLQEVWKWK AEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSATVTEAFVRLHEEGVIYRSTRLVNWSCTLNSAISDIEV DKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDPRYQHLK GKSVVHPFLSRSLPIVFDDFVDMEFGTGAVKITPAHDQNDYEVGQRHRLEAISIMDSKGALVNVPPPFLG LPRFEARKAVLAALKEQGLFRGIKDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRI LPEAHQRTWHSWMDNIRDWCISRQLWWGHRIPAYFITVHDPAVPPGEDPDGRYWVSGRTEAEAREKAARE FGVSPDKISLQQDEDVLDTWFSSGLFPFSIFGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKL TEKLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIHGVSLQGLHDQLLNSNLDPSEVEKAKEGQRADF PAGIPECGTDALRFGLCAYTSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSKPEGHE SLVDRWIRSRLAEAVRLSNEGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAADCARQTLY TCLDVGLRLLSPFMPFVTEELFQRLPRRTPNAPASLCVTPYPEPSECSWKDPEAEAALELALSITRAVRS LRADYNLTRTRPDCFLEVADEATGALASAVSAYVQTLASAGVVAVLALGAPAPQGCAVAVASDRCSIHLQ LQGLVDPARELGKLQAKRSEAQRQAQRLQERRAASGYSAKVPLEVQEADEVKLQQTEAELRKVDEAIALF QKML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053292
ORF Size	3792 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053292.1, NP_445744.1
RefSeq Size	3980 bp
RefSeq ORF	3795 bp
Locus ID	25009
UniProt ID	Q04462
MW	140.4 kDa
Gene Summary	catalyze the aminoacylation of tRNA by valine [RGD, Feb 2006]

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