Cacna1b (NM_147141) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	BIII
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207906 representing NM_147141 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGCG GCGGGGCCGGCGGGGCCGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA GTCCATTGCGCAACGCGCACGGACCATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATATGCTAAGCGCATCACCGAAT GGCCGCCCTTCGAATATATGATCCTGGCCACCATCATCGCCAACTGTATTGTCCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAGACTCCCATGTCTGAACGACTGGATGACACGGAACCTTACTTCATCGGCATC TTTTGCTTCGAGGCGGGCATCAAGATCATAGCTCTGGGCTTCGTGTTCCACAAAGGCTCCTACCTCCGGA ATGGCTGGAACGTCATGGACTTCGTGGTGGTCCTCACAGGGATTCTTGCCACAGCTGGAACTGACTTTGA TCTGCGCACCCTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGTTGGTGTCTGGAATTCCAAGCTTGCAG GTGGTGCTCAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATCGGGCTGCTGCTCTTCTTCGCCA TCCTCATGTTCGCTATCATCGGCCTCGAGTTCTATATGGGCAAATTCCATAAGGCCTGCTTCCCCAACAG CACAGATGCAGAGCCTGTGGGTGACTTTCCTTGTGGCAAGGAGGCCCCTGCTCGTCTGTGTGACAGTGAC ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA TCTTGACCGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC CGGCAACACGTGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG GTGCTCGGTGTGCTTTCAGGAGAGTTTGCCAAAGAGCGGGAGCGAGTCGAGAACCGCCGTGCCTTCCTGA AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAACTGAATGGGTACTTGGAGTGGATCTTCAAGGCGGAGGA AGTCATGTTGGCAGAGGAGGACAAGAACGCAGAAGAGAAGTCCCCTTTGGATGTGTTGAAGAGAGCTGCT ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCTG CTGGGTCTCCCTTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACAGAGAGCTCATCGTACTTCCGGAGGAA GGAGAAGATGTTCCGGTTCCTTATCCGTCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGC GTGGTGGCCCTGAACACGTTGTGTGTGGCCATGGTACACTATAATCAGCCTCAGCGGCTTACCACTGCAC TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTACGGTCTAGG GCCCAGAAGCTACTTCCGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTCGGAATCAGTGTGCTGCGGGCTCTCCGACTGCTGA GGATTTTCAAAGTCACCAAGTATTGGAACTCCCTGAGGAACCTGGTTGTTTCCCTCCTCAACTCCATGAA GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTTTTCATTGTGGTCTTCGCTCTGTTGGGGATGCAGCTG TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC TCACTGTGTTTCAGATTCTGACAGGAGAGGACTGGAATGCAGTCATGTATCATGGGATTGAGTCACAAGG AGGAGTCAGCAAAGGCATGTTTTCATCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG TTGAACGTTTTCTTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG AGATGGAAGAGGCAGCCAATCAGAAGCTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT GTCTGCTGCCAACATCTCCATTGCTGCCAGGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAG CGGGCCAGTCAGCTAAGGCTCCAGAACCTGCGTGCCAGCTGTGAGGCACTGTACAGTGAGATGGACCCGG AGGAGCGCCTGCGTTATGCCAGCACGCGCCACGTGAGGCCAGACATGAAGACACACATGGACCGACCCCT AGTGGTGGAACCTGGTCGGGATGGCCTGCGGGGACCCGCCGGGAACAAGTCAAAGCCTGAGGGCACGGAG GCCACCGAAGGTGCGGATCCACCACGCCGACACCACCGGCATCGTGATAGGGACAAGACCTCAGCCTCAA CCCCTGCTGGAGGCGAACAGGACAGGACAGACTGCCCAAAGGCCGAAAGCACCGAGACCGGGGCCCGGGA GGAACGTGCGCGCCCTCGTCGAAGTCACAGCAAGGAGGCTCCAGGGGCTGACACACAAGTGCGTTGTGAG CGCAGTAGACGTCACCACCGGCGCGGATCCCCGGAGGAGGCCACTGAACGGGAACCTCGGCGCCACCGTG CCCACCGGCACGCACAGGACTCAAGCAAGGAAGGCAAGGAGGGCACTGCACCGGTGCTTGTACCCAAGGG CGAGCGTCGCGCAAGACATCGAGGCCCGCGTACGGGCCCCCGTGAGACAGAGAACAGTGAGGAGCCCACA CGCAGGCACCGTGCAAAGCATAAGGTGCCACCAACACTTGAGCCCCCAGAGAGGGAGGTTGCAGAGAAGG AGAGCAACGTGGTGGAAGGGGATAAGGAAACTCGAAATCACCAGCCCAAGGAACCTCGCTGTGACCTGGA GGCCATTGCGGTTACAGGCGTGGGCTCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAA CAGCCAGAGGATGCAGACAACCAGCGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGACCCCAGCACCA CTGTGCATGTCCCAGTGACACTGACAGGCCCTCCCGGGGAGGCCACTGTAGTTCCCAGTGCTAACACGGA CCTGGAAGGCCAAGCGGAGGGCAAGAAGGAGGCAGAGGCTGACGATGTGCTGAGAAGAGGCCCCAGGCCC ATCGTTCCCTACAGTTCCATGTTCTGCCTCAGCCCCACCAACCTACTCCGTCGCTTCTGCCATTACATTG TGACCATGCGGTACTTTGAGATGGTGATTCTTGTGGTCATCGCCTTGAGCAGCATTGCCCTGGCTGCTGA GGATCCCGTGCGGACCGACTCATTCCGGAACAATGCTCTGAAGTACATGGACTACATCTTTACAGGAGTC TTCACCTTTGAGATGGTCATAAAGATGATAGACTTGGGCCTGCTGCTGCACCCTGGGGCCTACTTCCGGG ACCTGTGGAACATTCTGGACTTCATTGTTGTCAGTGGAGCCCTGGTGGCATTTGCATTCTCAGGATCCAA AGGGAAAGACATCAATACCATCAAGTCTCTGAGAGTCCTGCGAGTCCTGCGGCCCCTCAAGACCATCAAG CGGCTGCCTAAACTCAAGGCTGTGTTTGACTGTGTGGTGAACTCTCTGAAGAATGTCTTGAACATCCTGA TCGTCTACATGCTCTTCATGTTTATATTTGCCGTCATCGCCGTCCAACTCTTCAAAGGGAAGTTCTTTTA CTGCACTGATGAGTCCAAGGAGCTGGAGCGGGACTGCAGGGGTCAGTATTTGGATTATGAGAAGGAAGAG GTAGAAGCCCAGCCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTGCTCTGGGCCTTGCTGA CTCTGTTTACGGTGTCCACAGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGACGCCACCTATGA GGAGCAGGGGCCAAGCCCCGGGTTTCGGATGGAGCTTTCCATCTTCTATGTGGTCTACTTTGTGGTCTTC CCTTTTTTCTTTGTCAACATCTTTGTGGCCTTGATCATCATCACCTTCCAGGAGCAGGGGGACAAGGTGA TGTCTGAGTGCAGTCTGGAAAAGAATGAGAGGGCTTGCATTGACTTTGCCATCAGCGCCAAACCCCTGAC ACGGTACATGCCTCAGAACAAGCAGTCGTTCCAGTATAAGACATGGACATTTGTGGTCTCTCCACCCTTT GAGTACTTCATTATGGCCATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTACGATGCCCCTT ATGAGTACGAGCTGATGCTGAAGTGCTTGAACATCGTCTTCACATCCATGTTCTCTCTGGAGTGCATCCT GAAGATCATCGCCTTCGGGGTGTTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACTGTT TTGGGAAGTATTACTGATATTTTAGTAACGGAGATTGCGGAAACGAACAACTTCATCAACTTGAGCTTCC TTCGCCTCTTCCGGGCAGCACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCTTGTTATG GACCTTTGTCCAGTCCTTTAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTAC GCCATCATCGGCATGCAGGTTTTTGGAAACATTGCCCTTGATGATGGCACCAGCATCAACCGACACAACA ACTTCCGGACATTTCTGCAAGCCTTAATGCTGTTGTTCAGGAGTGCCACTGGGGAGGCCTGGCACGAAAT CATGCTGTCTTGCCTGGGCAACCGGGCCTGCGACCCACATGCCAACGCCAGCGAATGCGGGAGCGACTTT GCCTATTTTTATTTTGTCTCCTTCATCTTCCTCTGTTCCTTTCTGATGCTGAACCTCTTTGTTGCTGTGA TCATGGACAATTTCGAATACCTCACGCGGGATTCTTCCATCCTAGGGCCGCACCACCTCGATGAATTCAT TCGCGTCTGGGCTGAATACGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTG AAACACATGTCCCCACCTCTGGGTTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTC GAATGAACATGCCCATATCCAATGAGGACATGACGGTACACTTTACATCCACACTGATGGCCCTCATCCG GACGGCACTGGAGATCAAGCTTGCCCCAGCGGGGACAAAACAGCACCAATGTGATGCTGAGCTGAGGAAG GAGATCTCTTCTGTGTGGGCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTGCCACCCCACAAACCTG ACGAGATGACAGTGGGGAAGGTCTATGCGGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCAC CAGAGATCAGACTCACCAAGCTCCTGGAGGCCTGTCCCAGATGGGTCCTGTTTCCCTGTTCCATCCTCTG AAGGCCACCCTGGAGCAGACACAGCCCGCTGTGCTCCGAGGAGCTCGGGTTTTCCTTCGACAAAAGAGTG CAACTTCCCTCAGCAATGGGGGCGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCCCTGTCCTGGGG CACGCAGAGGACCCAGGACGTACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATC CCTGTGGGGCAGCCAGGAGCACTGGCTGTAGATGTCCAGATGCAGAACATGACATTGAGAGGACCGGATG GGGAGCCCCAGCCTGGCCTGGAGAGCCAAGGCCGAGCGGCCTCTATGCCACGCCTGGCGGCAGAAACACA GCCGGCCCCTAATGCCAGCCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCGCATGGGACTCAG CTTTGCAACACAGTCCTGGACCGGCCACCTCCTAGCCAGGTGTCCCATCACCACCACCACCGCTGCCACC GGCGCAGGGACAAGAAGCAGAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACACAGAAGGTGCACC AAGTACTGCTGCAGGATCTGGCCTGCCCCATGGAGAAGGGTCCACAGGCTGCCGGCGGGAGCGTAAGCAA GAGCGAGGCCGGTCCCAGGAGCGGAGGCAGCCCTCCTCCTCTTCTTCAGAGAAGCAGCGCTTCTATTCCT GTGACCGCTTTGGGAGCCGGGAGCCCCCACAACCTAAGCCCTCCCTCAGTAGCCACCCCATATCGCCAAC AGCGGCACTAGAGCCAGGACCCCACCCGCAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACA TCTGGTGCTAGCACGCCGGGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCCCTGACCCCACGCCCCA GCATCACCTACAAGACGGCCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGTGGCCTTCCAGCCTT CTCCCCTGGCCGTCTCAGCCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAG CCTCTAGCTTCTGGCTCCCGCATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCTG CCCACAACCTGCCTGAGGATACACTCACCTTTGAAGAGGCCGTGGCCACCAACTCTGGCCGCTCCTCCAG GACTTCCTATGTGTCCTCCCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTACCACTGC ACTTTGGGACTCAGCACCGGCGTCCGGGCGCGGCACAGCTACCACCACCCAGACCAGGATCACTGGTGCT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_147141
Insert Size	7002 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_147141.1, NP_671482.1
RefSeq Size	9695 bp
RefSeq ORF	7002 bp
Locus ID	257648

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