Cacna1b Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	WB: 1:200-1:2000; IHC: 1:100-1:3000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide (C)RHHRHRDRDKTSASTPA, corresponding to amino acid residues 851-867 of rat Cav2.2. Intracellular loop between domains II and III.
Formulation	Lyophilized. Concentration before lyophilization ~0.8mg/ml (lot dependent, please refer to CoA along with shipment for actual concentration). Buffer before lyophilization: phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Reconstitution Method	Add 50 ul double distilled water (DDW) to the lyophilized powder.
Purification	Affinity purified on immobilized antigen.
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	calcium voltage-gated channel subunit alpha1 B
Database Link	NP_671482 Entrez Gene 774 HumanEntrez Gene 12287 MouseEntrez Gene 257648 Rat
Background	Voltage dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.The voltage dependent Ca2+ channels are composed of several subunits; a1, Ã?, a2d and ?. CaV channels were originally divided into six physiological types: L, N, P, Q, R, and T type.The CaV2.2 (formally named a1B) composes the a1 poreforming subunit for the N type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, ?-aminobutyric acid, acetylcholine, dopamine and noradrenaline.The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding GÃ?? to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.?-Conotoxin GVIA is a specific blocker of Cav2.2 Ca2+ channels. It specifically blocks N-type Cav channels by binding to the Cav2.2 a1 subunit (a1B) and its action is only partially reversible.
Synonyms	BIII; CACH5; CACNL1A5; CACNN; Cav2.2
Reference Data

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