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Klhl25 (NM_001039006) Rat Untagged Clone
CAT＃: RN206142
Klhl25 (untagged ORF) - Rat kelch-like 25 (Drosophila) (Klhl25), (10 ug)
CN¥ 7,510.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1310815 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN206142 representing NM_001039006
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGTCAGCGTTCACGAGACCCGCAAGTCGCGGAGTAGCACAGGCTCCATGAACATCTCGGTCTTCC ACAAGGCCTCGCACCCTGACTGTGTGCTGGCCCACCTCAACACTCTGCGCAAGCACTGCATGTTCACCGA CGTCACTCTCTGGGCTGGTGACCGAGCCTTTCCTTGCCACCGTGCAGTGCTGGCTGCCTCCAGCCGATAC TTTGAGGCCATGTTCAGCCATGGCCTTCGGGAAAGCCGGGATGATACGGTCAACTTCCAGGACAACCTGC ACCCTGAGGTGCTGGAGCTGCTCCTGGACTTCGCCTACTCCTCTCGCATCGTCATCAATGAGGAGAACGC AGAGTCGCTGCTGGAGGCTGGTGACATGTTGCAGTTCCATGACGTCCGGGATGCAGCTGCCGAGTTCCTG GAGAAGAATCTTTCCCCCTCCAACTGCCTTGGCATGATGGTGCTGTCTGATGCTCACCAGTGCCGGCGGC TCTATGAGTTCTCATGCCGAATGTCCCTGGTACACTTTGAGACGGTGCGGCAGAGTGAGGACTTCAACAG TCTCTCTAGGGACACACTGTTGGACCTCATCTCCAGAGACGAGCTGGAGACTGAGGATGAGCGCGTGGTC TTTGAGGCCATCCTGCAGTGGGTGAAGCATGACCTAGAGCAGAGGAAGGTCCACCTGCCACTGCTCCTGC GCAATGTGCGACTGGCCCTGCTACCCTCAGACTGTCTGAAGAAAGCCGTCTCTGGAGAGGCCCTCCTCAT GGCGGATGAGTGCACCAAACTCATCATAGATGAGGCCTTCCGCTGCAAGACCAAGATCTTGCTGAACGAT GGTGTGGTCACCAGTCCCTTTGCCCGGCCTCGCAAGGCAGGTCACACATTGCTCATCCTGGGAGGCCAGA CCTTCATGTGTGACAAGATCTACCAAGTGGACCACAAAGCCAAGGAGATTATCCCCAAGGCTGATCTGCC GAGCCCTCGGAAGGAGTTCAGCGCTTCAGCGATTGGTTGCAAGGTCTACGTGACTGGAGGCAGGGGCTCT GAGAATGGGGTCTCTAAGGATGTCTGGGTGTATGACACTGTCCATGAGGAATGGTCTAAGGCAGCTCCCA TGCTGATTGCCCGCTTTGGCCATGGCTCAGCTGAGCTGGAAAACTGTCTCTATGTGGTAGGGGGGCATAC ATCCCTAGCAGGCATTTTCCCTGCCTCTCCTTCTGTCTCCCTGAAACAAGTTGAGAAATATGACCCTGGG GATAATAAGTGGACTATGGTGGCCCCAATGAGAGACGGTGTCAGCAATGCTGCTGTGGTGAGTGCCAAGC TGAAACTCTTTGTGTTTGGAGGGACCAGCATCCACCGGGACATGGTGTCCAAAGTCCAGTGTTTTGACCC CTCGGACAACCGGTGGACAATCAAGGCAGAATGTCCCCAGCCTTGGCGATACACAGCAGCTGCTGTTCTG GGCAGCCAGATTTTCATCATGGGAGGTGACACAGAGTACACAGCAGCTTCAGCATATCGCTTCGACTGTG AGACCAACCAGTGGACGCGGATTGGGGACATGACAGCCAAACGCATGTCCTGTCATGCTGTGGCTTCAGG CAACAAGCTGTATGTGGTTGGAGGCTACTTTGGGACCCAGAGGTGTAAGACCCTGGACTGCTATGACCCT ACTTCAGACACATGGAACTGCATCACCAGCGTGCCCTACTCTCTCATTCCCACGGCCTTTGTTAGCACCT GGAAGCATCTGCCTGCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001039006 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001039006.1, NP_001034095.1 |
| RefSeq Size | 3303 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 293023 |
| UniProt ID | Q4KLM4 |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR206142 | Klhl25 (Myc-DDK-tagged ORF) - Rat kelch-like 25 (Drosophila) (Klhl25), (10 ug) |
CN¥ 7,510.00 |
|
| RR206142L3 | Lenti ORF clone of Klhl25 (Myc-DDK-tagged ORF) - Rat kelch-like 25 (Drosophila) (Klhl25), (10 ug) |
CN¥ 9,410.00 |
|
| RR206142L4 | Lenti ORF clone of Klhl25 (mGFP-tagged ORF) - Rat kelch-like 25 (Drosophila) (Klhl25), (10 ug) |
CN¥ 10,360.00 |
