Agrn (NM_175754) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AGR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205800 representing NM_175754 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTCTGCCACTGGAACACAGACCCAGGCAGGAGCCTGGTGCCTCCATGCTGGTTCGATACTTCA TGATCCCCTGCAACATCTGCTTGATCCTGTTGGCCACTTCCACATTGGGCTTTGCGGTTCTGCTTTTCCT CAGCAACTACAAACCTGGAATCCACTTCACACCAGCGCCTCCTACGCCTCCTGATGTATGCAGGGGAATG TTATGTGGCTTTGGTGCTGTGTGTGAACCTAGTGTTGAGGATCCAGGCCGTGCCTCCTGTGTGTGCAAGA AGAATGCTTGCCCTGCTACGGTGGCTCCTGTGTGTGGCTCAGATGCCTCCACCTATAGCAACGAGTGTGA GCTGCAGCGCGCGCAGTGCAACCAGCAACGGCGCATCCGCCTGCTTCGCCAAGGGCCATGTGGGTCCCGG GACCCCTGTGCCAACGTGACCTGCAGTTTCGGTAGTACCTGTGTACCTTCGGCTGACGGACAGACTGCCT CATGTCTGTGTCCTACAACCTGCTTCGGGGCCCCTGATGGCACAGTGTGTGGCAGTGACGGTGTTGACTA CCCTAGTGAATGCCAGCTGCTTAGTCATGCCTGTGCCAGCCAGGAGCACATCTTCAAGAAGTTCAATGGT CCTTGTGATCCCTGCCAGGGCAGCATGTCAGACCTGAACCACATTTGCCGTGTGAACCCACGTACACGGC ACCCAGAAATGCTTCTGCGGCCTGAGAACTGCCCTGCCCAGCACACGCCTATCTGTGGAGATGATGGGGT CACCTATGAAAACGACTGTGTCATGAGCCGTATAGGTGCAACCCGTGGCCTGCTTCTCCAGAAAGTACGC TCTGGTCAATGCCAAACTCGAGACCAGTGCCCGGAGACCTGCCAGTTTAACTCCGTATGCCTGTCCCGCC GTGGCCGTCCCCACTGTTCCTGCGATCGTGTCACCTGTGATGGGTCTTACAGGCCTGTGTGTGCCCAAGA TGGGCACACATACAACAATGACTGTTGGCGCCAACAGGCTGAGTGTCGACAACAGCGGGCCATTCCTCCC AAGCACCAGGGCCCGTGTGACCAGACCCCGTCCCCATGCCATGGAGTGCAATGTGCATTTGGGGCAGTAT GCACAGTGAAGAACGGGAAAGCCGAGTGCGAGTGCCAGCGGGTGTGCTCCGGCATCTACGATCCTGTGTG CGGCAGTGACGGTGTCACTTACGGCAGTGTGTGCGAGCTGGAATCCATGGCCTGTACCCTTGGGCGGGAA ATCCAAGTGGCCCGCAGAGGACCCTGTGACCCATGTGGGCAGTGCCGTTTTGGATCCCTGTGCGAGGTGG AAACTGGACGCTGTGTGTGCCCCTCTGAGTGTGTGGAGTCAGCCCAGCCCGTATGCGGTTCTGATGGACA CACATACGCTAGTGAATGTGAGCTGCATGTCCACGCCTGTACGCACCAGATCAGCCTATACGTGGCCTCA GCTGGACACTGCCAGACCTGTGGAGAAAAAGTTTGTACTTTTGGGGCTGTGTGCTCAGCTGGACAGTGTG TATGTCCCCGTTGTGAGCACCCTCCACCTGGCCCTGTGTGTGGCAGTGATGGTGTCACCTACCTCAGTGC CTGTGAGCTACGCGAAGCTGCCTGTCAGCAGCAGGTACAAATTGAGGAGGCCCATGCAGGGCCATGTGAG CCGGCTGAGTGTGGCTCAGGGGGCTCTGGTTCTGGGGAGGACGACGAGTGTGAACAGGAGCTATGCCGGC AGCGTGGTGGTATCTGGGACGAGGACTCAGAAGATGGGCCATGTGTCTGTGACTTTAGCTGCCAGAGTGT CCCTAGAAGCCCAGTGTGTGGCTCGGATGGAGTCACCTATGGCACCGAGTGTGATCTGAAGAAGGCCAGG TGCGAATCACAGCAAGAACTGTACGTTGCTGCTCAGGGAGCCTGCCGTGGCCCTACCTTGGCTCCACTGC TACCTGTGGCCTTCCCACACTGTGCCCAAACCCCCTATGGCTGCTGCCAGGACAATTTCACTGCTGCCCA GGGTGTGGGCTTGGCTGGCTGTCCCAGCACCTGCCATTGCAACCCCCATGGCTCCTACAGTGGCACTTGT GACCCAGCCACAGGGCAGTGCTCCTGCCGACCAGGTGTAGGAGGCCTCAGGTGTGATCGCTGTGAACCTG GCTTCTGGAACTTCCGTGGCATCGTCACCGATGGACATAGTGGTTGCACTCCCTGCAGCTGCGACCCTCG GGGTGCCGTAAGGGATGACTGTGAACAGATGACTGGATTGTGTTCCTGTAGGCCTGGTGTGGCTGGTCCC AAGTGTGGACAGTGTCCAGATGGTCAAGTCCTGGGCCATCTAGGCTGTGAAGCAGATCCCATGACACCTG TGACTTGTGTGGAGATACACTGCGAGTTCGGGGCCTCCTGCGTAGAGAAGGCTGGTTTTGCCCAATGTAT CTGCCCAACCCTCACATGTCCAGAGGCTAACTCTACCAAGGTCTGCGGATCAGACGGTGTGACATATGGC AATGAATGCCAGCTGAAGGCCATTGCCTGCCGCCAGCGTCTGGACATCTCCACTCAGAGTCTTGGTCCCT GCCAGGAGAGTGTTACTCCTGGGGCTTCCCCAACATCTGCATCTATGACTACCCCAAGGCATATCCTGAG CAAGACGCTGCCATTTCCCCACAACAGCCTTCCTCTGTCTCCCGGCAGTACTACCCATGATTGGCCCACC CCATTACCCATATCACCTCACACCACAGTCAGCATCCCCAGAAGCACCGCGTGGCCTGTGCTGACCGTGC CCCCTACAGCAGCAGCCTCTGACGTAACCAGTCTCGCAACATCAATCTTCAGTGAATCCGGCAGCGCCAA TGGGAGTGGCGACGAGGAACTGAGTGGAGATGAGGAGGCCAGTGGGGGCGGGTCTGGGGGACTTGAGCCC CCGGTGGGCAGCATTGTGGTGACCCATGGGCCACCCATCGAGAGGGCTTCCTGCTACAACTCACCTTTGG GCTGCTGCTCAGATGGCAAGACACCCTCACTGGACTCCGAAGGCTCCAACTGTCCTGCTACCAAGGCATT CCAGGGCGTGCTGGAGCTTGAGGGGGTCGAGGGACAGGAACTGTTCTACACACCTGAGATGGCTGACCCC AAGTCAGAGTTGTTTGGGGAGACCGCAAGGAGCATTGAAAGCACGCTGGACGACCTGTTCCGGAATTCAG ACGTCAAGAAGGACTTCTGGAGTGTCCGCCTACGGGAACTGGGGCCTGGCAAGTTGGTCCGCGCCATTGT GGACGTTCACTTTGACCCTACCACAGCCTTCCAGGCATCAGATGTGGGTCAGGCCTTGCTCCGACAGATC CAGGTATCTAGGCCATGGGCCCTGGCAGTGAGGAGGCCTCTGCAGGAACATGTGCGATTCTTAGACTTTG ACTGGTTTCCCACTTTCTTTACGGGAGCTGCAACAGGAACTACAGCTGCTATGGCCACAGCCAGAGCCAC CACTGTGAGCCGACTGCCGGCCTCTTCCGTCACCCCACGAGTCTACCCCAGTCACACCAGCCGGCCTGTT GGCAGAACTACAGCACCACCAACCACTCGCCGGCCACCAACCACTGCCACCAACATGGACCGGCCTCGGA CCCCAGGCCATCAACAGCCCTCAAAGTCCTGTGATTCCCAACCTTGCCTCCATGGAGGTACCTGCCAGGA CCAGGATTCTGGCAAGGGTTTCACCTGCAGCTGTACTGCAGGCAGGGGTGGCTCTGTCTGTGAGAAAGTA CAACCCCCCTCCATGCCAGCTTTTAAGGGCCACTCCTTCTTGGCCTTCCCCACCCTCCGAGCCTACCACA CACTGCGCCTAGCACTGGAATTCCGGGCTTTGGAGACAGAGGGACTGCTACTCTACAATGGCAATGCACG TGGCAAAGATTTCCTGGCTCTGGCTCTGTTGGATGGGCGTGTGCAGTTCAGGTTTGACACAGGCTCAGGG CCAGCAGTGCTAACGAGCTTAGTGCCAGTGGAACCAGGACGGTGGCACCGCCTCGAGTTGTCACGGCATT GGCGTCAGGGCACACTTTCTGTGGATGGCGAGACTCCTGTTGTAGGTGAAAGTCCCAGTGGCACCGATGG CCTCAACTTGGACACGAACCTCTATGTGGGTGGTATCCCAGAAGAACAAGTTGCCATGGTGCTTGATCGG ACCTCTGTGGGCGTTGGCCTGAAAGGATGCATTCGTATGCTGGATATCAACAATCAGCAGCTGGAGCTTA GCGACTGGCAGAGGGCTGCGGTTCAAAGCTCTGGTGTGGGAGAATGTGGAGACCATCCGTGCTTACCTAA CCCCTGCCATGGTGGGGCCCTATGCCAGGCACTGGAGGCTGGCATGTTCCTCTGTCAGTGCCCGCCTGGC CGCTTTGGCCCAACTTGTGCAGATGAAAAGAGCCCCTGCCAGCCGAACCCCTGCCATGGGGCAGCCCCCT GTCGTGTGCTTTCCAGCGGTGGGGCCAAGTGCGAGTGCCCCCTGGGACGCAGTGGTACCTTCTGTCAGAC AGTCCTGGAGACTGCTGGCTCCCGGCCCTTCCTGGCTGACTTTAATGGCTTCTCCTACCTGGAACTGAAA GGCTTGCACACCTTCGAGAGAGACCTAGGGGAGAAGATGGCGCTGGAGATGGTGTTCTTGGCTCGTGGGC CCAGTGGCTTACTCCTCTACAATGGGCAGAAGACAGATGGCAAGGGGGACTTTGTATCCCTGGCCCTGCA TAACCGGCACCTAGAGTTCTGCTATGACCTTGGCAAGGGGGCTGCAGTCATCAGGAGCAAAGAGCCCATA GCCCTGGGAACCTGGGTCAGGGTATTCCTGGAACGAAATGGCCGCAAGGGTGCCCTTCAAGTGGGTGATG GTCCCCGTGTGCTAGGGGAATCTCCGAAATCCCGCAAGGTTCCGCACACCATGCTCAACCTCAAGGAGCC TCTCTATATTGGGGGAGCTCCTGACTTCAGCAAGCTGGCCAGGGGGGCTGCAGTGTCCTCTGGCTTCAGT GGTGTCATCCAGCTGGTGTCTCTAAGAGGCCATCAACTGCTGACTCAGGAGCATGTGTTGCGGGCAGTAG ATGTCTCACCTTTTGCAGACCACCCTTGTACCCAGGCCTTGGGCAACCCCTGCCTTAATGGGGGCTCGTG TGTCCCGAGGGAAGCCACTTATGAATGCCTGTGTCCTGGGGGCTTCTCTGGGCTGCACTGCGAAAAGGGG CTAGTTGAGAAGTCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAATACCTCA ATGCTGTGATTGAGAGTGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCTTACGCACTGAGGCCAC TCAGGGGCTGGTGCTGTGGATTGGCAAGGCTGCAGAACGTGCAGATTACATGGCCCTGGCCATCGTGGAC GGGCACCTGCAACTGAGCTATGACCTAGGTTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAACA CCAACCGCTGGCTTCGAATCAGGGCTCACAGGGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAAGC CCCTGTGACTGGATCTTCCCCATTGGGTGCCACGCAACTGGACACAGATGGAGCCCTGTGGCTTGGAGGC CTGCAGAAGCTTCCTGTGGGGCAGGCTCTACCCAAGGCCTATGGCACAGGTTTTGTGGGCTGTCTGCGGG ACGTGGTAGTGGGCCATCGCCAGTTGCATCTGCTGGAGGACGCTGTCACCAAACCAGAGCTAAGACCCTG CCCCACTCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175754
Insert Size	5823 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175754.1, NP_786930.1
RefSeq Size	7286 bp
RefSeq ORF	5823 bp
Locus ID	25592
UniProt ID	P25304
Gene Summary	may play a role in synapse development and regeneration [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.