Agrn (NM_175754) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AGR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205800 representing NM_175754 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCCTCTGCCACTGGAACACAGACCCAGGCAGGAGCCTGGTGCCTCCATGCTGGTTCGATACTTCA TGATCCCCTGCAACATCTGCTTGATCCTGTTGGCCACTTCCACATTGGGCTTTGCGGTTCTGCTTTTCCT CAGCAACTACAAACCTGGAATCCACTTCACACCAGCGCCTCCTACGCCTCCTGATGTATGCAGGGGAATG TTATGTGGCTTTGGTGCTGTGTGTGAACCTAGTGTTGAGGATCCAGGCCGTGCCTCCTGTGTGTGCAAGA AGAATGCTTGCCCTGCTACGGTGGCTCCTGTGTGTGGCTCAGATGCCTCCACCTATAGCAACGAGTGTGA GCTGCAGCGCGCGCAGTGCAACCAGCAACGGCGCATCCGCCTGCTTCGCCAAGGGCCATGTGGGTCCCGG GACCCCTGTGCCAACGTGACCTGCAGTTTCGGTAGTACCTGTGTACCTTCGGCTGACGGACAGACTGCCT CATGTCTGTGTCCTACAACCTGCTTCGGGGCCCCTGATGGCACAGTGTGTGGCAGTGACGGTGTTGACTA CCCTAGTGAATGCCAGCTGCTTAGTCATGCCTGTGCCAGCCAGGAGCACATCTTCAAGAAGTTCAATGGT CCTTGTGATCCCTGCCAGGGCAGCATGTCAGACCTGAACCACATTTGCCGTGTGAACCCACGTACACGGC ACCCAGAAATGCTTCTGCGGCCTGAGAACTGCCCTGCCCAGCACACGCCTATCTGTGGAGATGATGGGGT CACCTATGAAAACGACTGTGTCATGAGCCGTATAGGTGCAACCCGTGGCCTGCTTCTCCAGAAAGTACGC TCTGGTCAATGCCAAACTCGAGACCAGTGCCCGGAGACCTGCCAGTTTAACTCCGTATGCCTGTCCCGCC GTGGCCGTCCCCACTGTTCCTGCGATCGTGTCACCTGTGATGGGTCTTACAGGCCTGTGTGTGCCCAAGA TGGGCACACATACAACAATGACTGTTGGCGCCAACAGGCTGAGTGTCGACAACAGCGGGCCATTCCTCCC AAGCACCAGGGCCCGTGTGACCAGACCCCGTCCCCATGCCATGGAGTGCAATGTGCATTTGGGGCAGTAT GCACAGTGAAGAACGGGAAAGCCGAGTGCGAGTGCCAGCGGGTGTGCTCCGGCATCTACGATCCTGTGTG CGGCAGTGACGGTGTCACTTACGGCAGTGTGTGCGAGCTGGAATCCATGGCCTGTACCCTTGGGCGGGAA ATCCAAGTGGCCCGCAGAGGACCCTGTGACCCATGTGGGCAGTGCCGTTTTGGATCCCTGTGCGAGGTGG AAACTGGACGCTGTGTGTGCCCCTCTGAGTGTGTGGAGTCAGCCCAGCCCGTATGCGGTTCTGATGGACA CACATACGCTAGTGAATGTGAGCTGCATGTCCACGCCTGTACGCACCAGATCAGCCTATACGTGGCCTCA GCTGGACACTGCCAGACCTGTGGAGAAAAAGTTTGTACTTTTGGGGCTGTGTGCTCAGCTGGACAGTGTG TATGTCCCCGTTGTGAGCACCCTCCACCTGGCCCTGTGTGTGGCAGTGATGGTGTCACCTACCTCAGTGC CTGTGAGCTACGCGAAGCTGCCTGTCAGCAGCAGGTACAAATTGAGGAGGCCCATGCAGGGCCATGTGAG CCGGCTGAGTGTGGCTCAGGGGGCTCTGGTTCTGGGGAGGACGACGAGTGTGAACAGGAGCTATGCCGGC AGCGTGGTGGTATCTGGGACGAGGACTCAGAAGATGGGCCATGTGTCTGTGACTTTAGCTGCCAGAGTGT CCCTAGAAGCCCAGTGTGTGGCTCGGATGGAGTCACCTATGGCACCGAGTGTGATCTGAAGAAGGCCAGG TGCGAATCACAGCAAGAACTGTACGTTGCTGCTCAGGGAGCCTGCCGTGGCCCTACCTTGGCTCCACTGC TACCTGTGGCCTTCCCACACTGTGCCCAAACCCCCTATGGCTGCTGCCAGGACAATTTCACTGCTGCCCA GGGTGTGGGCTTGGCTGGCTGTCCCAGCACCTGCCATTGCAACCCCCATGGCTCCTACAGTGGCACTTGT GACCCAGCCACAGGGCAGTGCTCCTGCCGACCAGGTGTAGGAGGCCTCAGGTGTGATCGCTGTGAACCTG GCTTCTGGAACTTCCGTGGCATCGTCACCGATGGACATAGTGGTTGCACTCCCTGCAGCTGCGACCCTCG GGGTGCCGTAAGGGATGACTGTGAACAGATGACTGGATTGTGTTCCTGTAGGCCTGGTGTGGCTGGTCCC AAGTGTGGACAGTGTCCAGATGGTCAAGTCCTGGGCCATCTAGGCTGTGAAGCAGATCCCATGACACCTG TGACTTGTGTGGAGATACACTGCGAGTTCGGGGCCTCCTGCGTAGAGAAGGCTGGTTTTGCCCAATGTAT CTGCCCAACCCTCACATGTCCAGAGGCTAACTCTACCAAGGTCTGCGGATCAGACGGTGTGACATATGGC AATGAATGCCAGCTGAAGGCCATTGCCTGCCGCCAGCGTCTGGACATCTCCACTCAGAGTCTTGGTCCCT GCCAGGAGAGTGTTACTCCTGGGGCTTCCCCAACATCTGCATCTATGACTACCCCAAGGCATATCCTGAG CAAGACGCTGCCATTTCCCCACAACAGCCTTCCTCTGTCTCCCGGCAGTACTACCCATGATTGGCCCACC CCATTACCCATATCACCTCACACCACAGTCAGCATCCCCAGAAGCACCGCGTGGCCTGTGCTGACCGTGC CCCCTACAGCAGCAGCCTCTGACGTAACCAGTCTCGCAACATCAATCTTCAGTGAATCCGGCAGCGCCAA TGGGAGTGGCGACGAGGAACTGAGTGGAGATGAGGAGGCCAGTGGGGGCGGGTCTGGGGGACTTGAGCCC CCGGTGGGCAGCATTGTGGTGACCCATGGGCCACCCATCGAGAGGGCTTCCTGCTACAACTCACCTTTGG GCTGCTGCTCAGATGGCAAGACACCCTCACTGGACTCCGAAGGCTCCAACTGTCCTGCTACCAAGGCATT CCAGGGCGTGCTGGAGCTTGAGGGGGTCGAGGGACAGGAACTGTTCTACACACCTGAGATGGCTGACCCC AAGTCAGAGTTGTTTGGGGAGACCGCAAGGAGCATTGAAAGCACGCTGGACGACCTGTTCCGGAATTCAG ACGTCAAGAAGGACTTCTGGAGTGTCCGCCTACGGGAACTGGGGCCTGGCAAGTTGGTCCGCGCCATTGT GGACGTTCACTTTGACCCTACCACAGCCTTCCAGGCATCAGATGTGGGTCAGGCCTTGCTCCGACAGATC CAGGTATCTAGGCCATGGGCCCTGGCAGTGAGGAGGCCTCTGCAGGAACATGTGCGATTCTTAGACTTTG ACTGGTTTCCCACTTTCTTTACGGGAGCTGCAACAGGAACTACAGCTGCTATGGCCACAGCCAGAGCCAC CACTGTGAGCCGACTGCCGGCCTCTTCCGTCACCCCACGAGTCTACCCCAGTCACACCAGCCGGCCTGTT GGCAGAACTACAGCACCACCAACCACTCGCCGGCCACCAACCACTGCCACCAACATGGACCGGCCTCGGA CCCCAGGCCATCAACAGCCCTCAAAGTCCTGTGATTCCCAACCTTGCCTCCATGGAGGTACCTGCCAGGA CCAGGATTCTGGCAAGGGTTTCACCTGCAGCTGTACTGCAGGCAGGGGTGGCTCTGTCTGTGAGAAAGTA CAACCCCCCTCCATGCCAGCTTTTAAGGGCCACTCCTTCTTGGCCTTCCCCACCCTCCGAGCCTACCACA CACTGCGCCTAGCACTGGAATTCCGGGCTTTGGAGACAGAGGGACTGCTACTCTACAATGGCAATGCACG TGGCAAAGATTTCCTGGCTCTGGCTCTGTTGGATGGGCGTGTGCAGTTCAGGTTTGACACAGGCTCAGGG CCAGCAGTGCTAACGAGCTTAGTGCCAGTGGAACCAGGACGGTGGCACCGCCTCGAGTTGTCACGGCATT GGCGTCAGGGCACACTTTCTGTGGATGGCGAGACTCCTGTTGTAGGTGAAAGTCCCAGTGGCACCGATGG CCTCAACTTGGACACGAACCTCTATGTGGGTGGTATCCCAGAAGAACAAGTTGCCATGGTGCTTGATCGG ACCTCTGTGGGCGTTGGCCTGAAAGGATGCATTCGTATGCTGGATATCAACAATCAGCAGCTGGAGCTTA GCGACTGGCAGAGGGCTGCGGTTCAAAGCTCTGGTGTGGGAGAATGTGGAGACCATCCGTGCTTACCTAA CCCCTGCCATGGTGGGGCCCTATGCCAGGCACTGGAGGCTGGCATGTTCCTCTGTCAGTGCCCGCCTGGC CGCTTTGGCCCAACTTGTGCAGATGAAAAGAGCCCCTGCCAGCCGAACCCCTGCCATGGGGCAGCCCCCT GTCGTGTGCTTTCCAGCGGTGGGGCCAAGTGCGAGTGCCCCCTGGGACGCAGTGGTACCTTCTGTCAGAC AGTCCTGGAGACTGCTGGCTCCCGGCCCTTCCTGGCTGACTTTAATGGCTTCTCCTACCTGGAACTGAAA GGCTTGCACACCTTCGAGAGAGACCTAGGGGAGAAGATGGCGCTGGAGATGGTGTTCTTGGCTCGTGGGC CCAGTGGCTTACTCCTCTACAATGGGCAGAAGACAGATGGCAAGGGGGACTTTGTATCCCTGGCCCTGCA TAACCGGCACCTAGAGTTCTGCTATGACCTTGGCAAGGGGGCTGCAGTCATCAGGAGCAAAGAGCCCATA GCCCTGGGAACCTGGGTCAGGGTATTCCTGGAACGAAATGGCCGCAAGGGTGCCCTTCAAGTGGGTGATG GTCCCCGTGTGCTAGGGGAATCTCCGAAATCCCGCAAGGTTCCGCACACCATGCTCAACCTCAAGGAGCC TCTCTATATTGGGGGAGCTCCTGACTTCAGCAAGCTGGCCAGGGGGGCTGCAGTGTCCTCTGGCTTCAGT GGTGTCATCCAGCTGGTGTCTCTAAGAGGCCATCAACTGCTGACTCAGGAGCATGTGTTGCGGGCAGTAG ATGTCTCACCTTTTGCAGACCACCCTTGTACCCAGGCCTTGGGCAACCCCTGCCTTAATGGGGGCTCGTG TGTCCCGAGGGAAGCCACTTATGAATGCCTGTGTCCTGGGGGCTTCTCTGGGCTGCACTGCGAAAAGGGG CTAGTTGAGAAGTCAGTGGGGGACCTAGAAACACTGGCCTTTGATGGGCGGACCTACATCGAATACCTCA ATGCTGTGATTGAGAGTGAGAAGGCACTGCAGAGCAACCACTTTGAACTGAGCTTACGCACTGAGGCCAC TCAGGGGCTGGTGCTGTGGATTGGCAAGGCTGCAGAACGTGCAGATTACATGGCCCTGGCCATCGTGGAC GGGCACCTGCAACTGAGCTATGACCTAGGTTCCCAGCCAGTTGTGCTGCGCTCCACTGTGAAGGTCAACA CCAACCGCTGGCTTCGAATCAGGGCTCACAGGGAGCACAGGGAAGGTTCCCTTCAGGTGGGCAATGAAGC CCCTGTGACTGGATCTTCCCCATTGGGTGCCACGCAACTGGACACAGATGGAGCCCTGTGGCTTGGAGGC CTGCAGAAGCTTCCTGTGGGGCAGGCTCTACCCAAGGCCTATGGCACAGGTTTTGTGGGCTGTCTGCGGG ACGTGGTAGTGGGCCATCGCCAGTTGCATCTGCTGGAGGACGCTGTCACCAAACCAGAGCTAAGACCCTG CCCCACTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205800 representing NM_175754 Red=Cloning site Green=Tags(s) MPPLPLEHRPRQEPGASMLVRYFMIPCNICLILLATSTLGFAVLLFLSNYKPGIHFTPAPPTPPDVCRGM LCGFGAVCEPSVEDPGRASCVCKKNACPATVAPVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSR DPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGTVCGSDGVDYPSECQLLSHACASQEHIFKKFNG PCDPCQGSMSDLNHICRVNPRTRHPEMLLRPENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVR SGQCQTRDQCPETCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPP KHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGRE IQVARRGPCDPCGQCRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVAS AGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCE PAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKAR CESQQELYVAAQGACRGPTLAPLLPVAFPHCAQTPYGCCQDNFTAAQGVGLAGCPSTCHCNPHGSYSGTC DPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGP KCGQCPDGQVLGHLGCEADPMTPVTCVEIHCEFGASCVEKAGFAQCICPTLTCPEANSTKVCGSDGVTYG NECQLKAIACRQRLDISTQSLGPCQESVTPGASPTSASMTTPRHILSKTLPFPHNSLPLSPGSTTHDWPT PLPISPHTTVSIPRSTAWPVLTVPPTAAASDVTSLATSIFSESGSANGSGDEELSGDEEASGGGSGGLEP PVGSIVVTHGPPIERASCYNSPLGCCSDGKTPSLDSEGSNCPATKAFQGVLELEGVEGQELFYTPEMADP KSELFGETARSIESTLDDLFRNSDVKKDFWSVRLRELGPGKLVRAIVDVHFDPTTAFQASDVGQALLRQI QVSRPWALAVRRPLQEHVRFLDFDWFPTFFTGAATGTTAAMATARATTVSRLPASSVTPRVYPSHTSRPV GRTTAPPTTRRPPTTATNMDRPRTPGHQQPSKSCDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCEKV QPPSMPAFKGHSFLAFPTLRAYHTLRLALEFRALETEGLLLYNGNARGKDFLALALLDGRVQFRFDTGSG PAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDR TSVGVGLKGCIRMLDINNQQLELSDWQRAAVQSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPG RFGPTCADEKSPCQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELK GLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFCYDLGKGAAVIRSKEPI ALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPDFSKLARGAAVSSGFS GVIQLVSLRGHQLLTQEHVLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKG LVEKSVGDLETLAFDGRTYIEYLNAVIESEKALQSNHFELSLRTEATQGLVLWIGKAAERADYMALAIVD GHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGG LQKLPVGQALPKAYGTGFVGCLRDVVVGHRQLHLLEDAVTKPELRPCPTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175754
ORF Size	5820 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175754.1, NP_786930.1
RefSeq Size	7286 bp
RefSeq ORF	5823 bp
Locus ID	25592
UniProt ID	P25304
MW	206.6 kDa
Gene Summary	may play a role in synapse development and regeneration [RGD, Feb 2006]

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