Nid2 (NM_001012005) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NID-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205786 representing NM_001012005 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGGGACCCGACAGCCGGGCGGCTGGCACCGCCATCACCGCTATCGCTGCTCCTGGTGCTGGTGC TGCTGTCACGAGTCGGGGCTCTGCGTCCTGAAGAGCTCTTTCCTTACGGGGAGTCGTGGGGAGACCGACT GCTGCCGGAGGGTGACGATGAAAGCTCAGCGGCTGTGAAGCTAGCAGTACCTCTACGCTTCTACGATGCC CAGTTCAGCAACCTCTACGTGGGCACCAACGGCATCATCTCCACCCAGGACTTCCCCAGGGAGACTCAAT ATGTAGATGATGATTTTCCTACTGACTTCCCAGCCATCGCCCCATTCCTGGCTGACATCGACACTAGTCA CAGCAGGGGCCGGATCCTGTACCGCGAGGACACCTCCCAGGCTGTGCTGAGTCTGGCTGCCCGCTACGTG CGCACAGGCTTCCCGCTGACCGGGTCCAGCTTCACCCCTACCCACGCCTTCCTGGCCACCTGGGAGCACG TGGGCGCCTATGAGGAGGTCAGGCGCGGGGCTGCGCCATCCGGAGAGCTGAACACTTTCCAGGCCGTTCT GGCATCCGATGAGTCTGATACCTACGCCCTCTTTCTCTATCCTGCCAATGGTCTTCAGTTCTTTGGAACT CGCCCCAAAGAGTCCTACAATGTCCAGCTGCAGCTTCCTGCCAGGGTGGGCTTCTGCCGAGGGGAAGCAG ACGACCTGAAGAGAGAAGCGCTGTATTTCAGCCTGACTAACACTGAGCAGTCGGTGAAGAATCTCTACCA ACTAAGCAACCTGGGGATTCCTGGAGCGTGGGCCTTTCACATCGGCAGCAGGCTGGCCCTGGACAATGTC CGGCCAGCCACAGTTGGAGGTGACCATTCCACAGCCCGCTCCTCAGCGCTGGAGCACTCCTTCAACCATG CTGCAGCCCTGGAGAGCTATACTGAGGACAGTTTCGATTACTACAATGAGAATGAAGAGGATGTAGAATA CCCACCCATTGAACCAGGGGAGGCCCTGGAAGGCCACAGCAGAATCGATGTGTCTTTCAATTCAGAGGTT AATCCCACGTCTCCAGATTCTGATCACGCTTCTCCTTTGCCACACCCAGCACCTGGTAACTGGCCATCCT ACCGGGAAACAGAATCGGCTTCTTTGGACCCTCAAACCAAACAGGGGCCACCTGTGGGAGAGGTAGAGGT CCTGGATTTCAAGGACCCAGCAGAACTTTTGGATCAGACGGGCACCAGAACCCCAGCTCCTCCAGGGGCA GATGCAGCTTTCCTAACTCCAGGCAGGGAAGACCTTGGGAACAGAGACACCCAGTCCTATCCAGAGGCAC GGCCAGTGCCTTCAGAGCCAGATGTTCCTGTCGCTCCTCTGGAAAGAGAAATCCTTCCTAATTACCCCGA GTCTGGTCACGTGCCACCCCTGAGTGGAGGGAGGTATGTGGTAGGACTGGAGGACCACGTGAGTTCTAAA GATCAAGTCTTCACTTACAATGGCGCCAACCGGGAAACCTGTGAACACAGCCACGGCCAGTGCTCCCGGC ATGCCTTCTGCACCGACTACACCACTGGCTTTTGCTGCCACTGCCAGTCTAGGTTTTATGGAAACGGGAG GCACTGCGTGCCAGAAGGGGCGCCTCACCGAGTGAATGGGAAGGTGAGTGGCCATCTCCAAGTGGGCCAC ATGCCTGTGCACTTCACGGATGTGGATCTACACGCTTACATCGTGAGCAATGACGGCAGAGCCTACACTG CCATCAGTCAGATCCCGCAGCCCGCAGCCCAGGCCCTCCTCCCTGTCCTGCCCATCGGAGGCCTGTTTGG CTGGCTCTTTGCTTTGGAGAAGCCAGGCTCTGAGAATGGCTTCAGCCTCACAGGAGCTACCTTTGTCCAT GATGTGGAAGTCACTTTTCACCCTGGAGAGGAGAGAGTTCGTATCACTCAAACCGCCGAGGGGCTTGACC CAGAGAACTATCTGAGCCTTAACACCAACATCGAAGGCCAGGTGCCCTTTATCCCAGCAAATTTCACAGC CCACATTGCTCCGTACCAAGAGTTCTATCATCACAGAGACTCAGTCGTGACATCCTCCAGCTCCCGAAGT TTCTCCCTCATTTCTGGTTCTATCAACCAAACTCGGTCCTACCGCATCGACCAGAACATCACTTACAAGG CGTGCAGTCATGCCCCCAGACACCTGGCCGTCCCTGCCACCCAGCAGCTGACTGTGGACCGGGCCTTTGC TTTGTACAGTGAGGAGGAGGGCGTGCTGAGGTTTGCCGTGACCAATCAGATTGGCCCTGTCGAAGTGGAC TCGGCCCCTACTGCAGTGAATCCTTGCTATGACGGGAGCCACACCTGTGACACAACAGCAAGGTGCCACC CGGGGACGGGTGTGGACTACACCTGCGAGTGCACGCCCGGGTTCCAGGGTGACGGACGGAGCTGTGTGGA TGTTAATGAGTGTGCCACTGGCTTCCATCGCTGCGGCCCCAATTCTGTGTGTGTCAACTTGGTGGGGAGC TACAGGTGCGAGTGCCGCAGCGGCTATGAATTCGCAGATGACCGGCACACTTGTGTCTTGATCGCCCCAC CTCCCAACCCTTGCCTGGATGGCAGTCACACCTGTGCCCCTGAGGGGCAGGCCCGGTGCATTCACCATGG AGGCAGTTCGTTCAGCTGTGCCTGCCTGCCCGGCTTTGTTGGCACCGGGCACCAGTGTTCTGATGTTGAC GAATGTGCGGAAAACAGATGTCATGGGGCAGCCATCTGTTACAATACCCCCGGGTCCTTCTCCTGCCGGT GCCAGCCTGGGTACCACGGGGACGGGTTTCACTGCGCCTCTGACACAGTTCCAGAAGATTCCATCTCAGG ACTGAAACCCTGTGAATACCAGCAGCGCTATGCCCAGGCACAGCATGCCCACGCTGGGTCACGGATCCAC ATCCCCCAGTGCGACGACCAGGGAAACTTTGTGCCGCTGCAGTGCCATGGCAGCACTGGCTTCTGCTGGT GTGTGGACCAAAATGGCCATGAAGTCCCTGGCACCCAGACTCCACCTGGCTCCACTCCGCCCCACTGTGG ACCACCTCCAGAGCCCACCCAGAGGCCTCGGACAGTCTGTGAGCGCTGGAGGGAAAGCCTGCTGGAACAC TACGGGGGCACACCCAGGGACGACCAGTATGTGCCCCAGTGTGACGACCTGGGCCACTTCATCCCCCTGC AGTGTCACGGGAAGAGTGATTTCTGTTGGTGTGTGGACAAGGATGGCAGAGAACTTCCAGGCACCCGCTC CCAGCCAGGCACCATGCCTGCATGCATACCCACTGTCGCCCCACCCGTGGTCCGGCCCACACCCCGGCCT GATGTGACTCCTCCGGCTGTGGGCACCTTCCTGCTCTATGCCCAGGGCCAGCAGATCGGTCACTTGCCCC TCAATGGCAGCAGGCTTCAGAAGGATGCAGCCAGGACCCTGCTGTCACTACATGGCTCCATAGTCGTGGG GATTGACTATGACTGCCGGGAGAGGATGGTCTACTGGACAGACGTTGCCGGGAGGACCATCAGCCGTGCC AGCTTGGAGGCAGGAGCCGAACCCGAGACCATCATTACCTCAGGTCTGATAAGCCCAGAAGGACTTGCCA TCGACCACTTCCGGAGAACGATGTACTGGACAGACAGCGGCCTGGATAAGATAGAGCGGGCTGGACTGGA CGGTTCCGAGCGTAAGGTCCTCTTCCACACAGATCTGGTGAATCCACGAGCTATCACTGTGGATCCAATC CGAGGCAACTTGTACTGGACAGACTGGAATAGAGAAGCTCCTAAAATTGAAACATCATCTTTAGATGGTG AAAACAGAAGAATTCTGATCAACAAAGATATTGGATTACCCAATGGATTGACCTTTGACCCCTTCTCCAA ACTTCTCTGCTGGGCAGATGCAGGAACCAAAAAACTGGAGTGTACACTACCTGATGGAACTGGACGCCGC GTCATCCAAAACCACCTCAATTACCCCTTCAGTGTTGTCAGTTACGCGGATCACTTCTACCACACGGACT GGCGGAGGGATGGTGTTATATCAGTGAGTAAAGACAGTGGCCAGCTTACTGATGAGTATCTTCCTGAACA GCGCTCTCACCTCTATGGCATCACTGCAGTCTATCCCTACTGTCCAACAGGAAGAAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001012005
Insert Size	4191 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001012005.2, NP_001012005.2
RefSeq Size	4782 bp
RefSeq ORF	4191 bp
Locus ID	302248
UniProt ID	B5DFC9
Gene Summary	Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions (By similarity).[UniProtKB/Swiss-Prot Function]

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