Nid2 (NM_001012005) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NID-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205786 representing NM_001012005 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTGGGACCCGACAGCCGGGCGGCTGGCACCGCCATCACCGCTATCGCTGCTCCTGGTGCTGGTGC TGCTGTCACGAGTCGGGGCTCTGCGTCCTGAAGAGCTCTTTCCTTACGGGGAGTCGTGGGGAGACCGACT GCTGCCGGAGGGTGACGATGAAAGCTCAGCGGCTGTGAAGCTAGCAGTACCTCTACGCTTCTACGATGCC CAGTTCAGCAACCTCTACGTGGGCACCAACGGCATCATCTCCACCCAGGACTTCCCCAGGGAGACTCAAT ATGTAGATGATGATTTTCCTACTGACTTCCCAGCCATCGCCCCATTCCTGGCTGACATCGACACTAGTCA CAGCAGGGGCCGGATCCTGTACCGCGAGGACACCTCCCAGGCTGTGCTGAGTCTGGCTGCCCGCTACGTG CGCACAGGCTTCCCGCTGACCGGGTCCAGCTTCACCCCTACCCACGCCTTCCTGGCCACCTGGGAGCACG TGGGCGCCTATGAGGAGGTCAGGCGCGGGGCTGCGCCATCCGGAGAGCTGAACACTTTCCAGGCCGTTCT GGCATCCGATGAGTCTGATACCTACGCCCTCTTTCTCTATCCTGCCAATGGTCTTCAGTTCTTTGGAACT CGCCCCAAAGAGTCCTACAATGTCCAGCTGCAGCTTCCTGCCAGGGTGGGCTTCTGCCGAGGGGAAGCAG ACGACCTGAAGAGAGAAGCGCTGTATTTCAGCCTGACTAACACTGAGCAGTCGGTGAAGAATCTCTACCA ACTAAGCAACCTGGGGATTCCTGGAGCGTGGGCCTTTCACATCGGCAGCAGGCTGGCCCTGGACAATGTC CGGCCAGCCACAGTTGGAGGTGACCATTCCACAGCCCGCTCCTCAGCGCTGGAGCACTCCTTCAACCATG CTGCAGCCCTGGAGAGCTATACTGAGGACAGTTTCGATTACTACAATGAGAATGAAGAGGATGTAGAATA CCCACCCATTGAACCAGGGGAGGCCCTGGAAGGCCACAGCAGAATCGATGTGTCTTTCAATTCAGAGGTT AATCCCACGTCTCCAGATTCTGATCACGCTTCTCCTTTGCCACACCCAGCACCTGGTAACTGGCCATCCT ACCGGGAAACAGAATCGGCTTCTTTGGACCCTCAAACCAAACAGGGGCCACCTGTGGGAGAGGTAGAGGT CCTGGATTTCAAGGACCCAGCAGAACTTTTGGATCAGACGGGCACCAGAACCCCAGCTCCTCCAGGGGCA GATGCAGCTTTCCTAACTCCAGGCAGGGAAGACCTTGGGAACAGAGACACCCAGTCCTATCCAGAGGCAC GGCCAGTGCCTTCAGAGCCAGATGTTCCTGTCGCTCCTCTGGAAAGAGAAATCCTTCCTAATTACCCCGA GTCTGGTCACGTGCCACCCCTGAGTGGAGGGAGGTATGTGGTAGGACTGGAGGACCACGTGAGTTCTAAA GATCAAGTCTTCACTTACAATGGCGCCAACCGGGAAACCTGTGAACACAGCCACGGCCAGTGCTCCCGGC ATGCCTTCTGCACCGACTACACCACTGGCTTTTGCTGCCACTGCCAGTCTAGGTTTTATGGAAACGGGAG GCACTGCGTGCCAGAAGGGGCGCCTCACCGAGTGAATGGGAAGGTGAGTGGCCATCTCCAAGTGGGCCAC ATGCCTGTGCACTTCACGGATGTGGATCTACACGCTTACATCGTGAGCAATGACGGCAGAGCCTACACTG CCATCAGTCAGATCCCGCAGCCCGCAGCCCAGGCCCTCCTCCCTGTCCTGCCCATCGGAGGCCTGTTTGG CTGGCTCTTTGCTTTGGAGAAGCCAGGCTCTGAGAATGGCTTCAGCCTCACAGGAGCTACCTTTGTCCAT GATGTGGAAGTCACTTTTCACCCTGGAGAGGAGAGAGTTCGTATCACTCAAACCGCCGAGGGGCTTGACC CAGAGAACTATCTGAGCCTTAACACCAACATCGAAGGCCAGGTGCCCTTTATCCCAGCAAATTTCACAGC CCACATTGCTCCGTACCAAGAGTTCTATCATCACAGAGACTCAGTCGTGACATCCTCCAGCTCCCGAAGT TTCTCCCTCATTTCTGGTTCTATCAACCAAACTCGGTCCTACCGCATCGACCAGAACATCACTTACAAGG CGTGCAGTCATGCCCCCAGACACCTGGCCGTCCCTGCCACCCAGCAGCTGACTGTGGACCGGGCCTTTGC TTTGTACAGTGAGGAGGAGGGCGTGCTGAGGTTTGCCGTGACCAATCAGATTGGCCCTGTCGAAGTGGAC TCGGCCCCTACTGCAGTGAATCCTTGCTATGACGGGAGCCACACCTGTGACACAACAGCAAGGTGCCACC CGGGGACGGGTGTGGACTACACCTGCGAGTGCACGCCCGGGTTCCAGGGTGACGGACGGAGCTGTGTGGA TGTTAATGAGTGTGCCACTGGCTTCCATCGCTGCGGCCCCAATTCTGTGTGTGTCAACTTGGTGGGGAGC TACAGGTGCGAGTGCCGCAGCGGCTATGAATTCGCAGATGACCGGCACACTTGTGTCTTGATCGCCCCAC CTCCCAACCCTTGCCTGGATGGCAGTCACACCTGTGCCCCTGAGGGGCAGGCCCGGTGCATTCACCATGG AGGCAGTTCGTTCAGCTGTGCCTGCCTGCCCGGCTTTGTTGGCACCGGGCACCAGTGTTCTGATGTTGAC GAATGTGCGGAAAACAGATGTCATGGGGCAGCCATCTGTTACAATACCCCCGGGTCCTTCTCCTGCCGGT GCCAGCCTGGGTACCACGGGGACGGGTTTCACTGCGCCTCTGACACAGTTCCAGAAGATTCCATCTCAGG ACTGAAACCCTGTGAATACCAGCAGCGCTATGCCCAGGCACAGCATGCCCACGCTGGGTCACGGATCCAC ATCCCCCAGTGCGACGACCAGGGAAACTTTGTGCCGCTGCAGTGCCATGGCAGCACTGGCTTCTGCTGGT GTGTGGACCAAAATGGCCATGAAGTCCCTGGCACCCAGACTCCACCTGGCTCCACTCCGCCCCACTGTGG ACCACCTCCAGAGCCCACCCAGAGGCCTCGGACAGTCTGTGAGCGCTGGAGGGAAAGCCTGCTGGAACAC TACGGGGGCACACCCAGGGACGACCAGTATGTGCCCCAGTGTGACGACCTGGGCCACTTCATCCCCCTGC AGTGTCACGGGAAGAGTGATTTCTGTTGGTGTGTGGACAAGGATGGCAGAGAACTTCCAGGCACCCGCTC CCAGCCAGGCACCATGCCTGCATGCATACCCACTGTCGCCCCACCCGTGGTCCGGCCCACACCCCGGCCT GATGTGACTCCTCCGGCTGTGGGCACCTTCCTGCTCTATGCCCAGGGCCAGCAGATCGGTCACTTGCCCC TCAATGGCAGCAGGCTTCAGAAGGATGCAGCCAGGACCCTGCTGTCACTACATGGCTCCATAGTCGTGGG GATTGACTATGACTGCCGGGAGAGGATGGTCTACTGGACAGACGTTGCCGGGAGGACCATCAGCCGTGCC AGCTTGGAGGCAGGAGCCGAACCCGAGACCATCATTACCTCAGGTCTGATAAGCCCAGAAGGACTTGCCA TCGACCACTTCCGGAGAACGATGTACTGGACAGACAGCGGCCTGGATAAGATAGAGCGGGCTGGACTGGA CGGTTCCGAGCGTAAGGTCCTCTTCCACACAGATCTGGTGAATCCACGAGCTATCACTGTGGATCCAATC CGAGGCAACTTGTACTGGACAGACTGGAATAGAGAAGCTCCTAAAATTGAAACATCATCTTTAGATGGTG AAAACAGAAGAATTCTGATCAACAAAGATATTGGATTACCCAATGGATTGACCTTTGACCCCTTCTCCAA ACTTCTCTGCTGGGCAGATGCAGGAACCAAAAAACTGGAGTGTACACTACCTGATGGAACTGGACGCCGC GTCATCCAAAACCACCTCAATTACCCCTTCAGTGTTGTCAGTTACGCGGATCACTTCTACCACACGGACT GGCGGAGGGATGGTGTTATATCAGTGAGTAAAGACAGTGGCCAGCTTACTGATGAGTATCTTCCTGAACA GCGCTCTCACCTCTATGGCATCACTGCAGTCTATCCCTACTGTCCAACAGGAAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205786 representing NM_001012005 Red=Cloning site Green=Tags(s) MSWDPTAGRLAPPSPLSLLLVLVLLSRVGALRPEELFPYGESWGDRLLPEGDDESSAAVKLAVPLRFYDA QFSNLYVGTNGIISTQDFPRETQYVDDDFPTDFPAIAPFLADIDTSHSRGRILYREDTSQAVLSLAARYV RTGFPLTGSSFTPTHAFLATWEHVGAYEEVRRGAAPSGELNTFQAVLASDESDTYALFLYPANGLQFFGT RPKESYNVQLQLPARVGFCRGEADDLKREALYFSLTNTEQSVKNLYQLSNLGIPGAWAFHIGSRLALDNV RPATVGGDHSTARSSALEHSFNHAAALESYTEDSFDYYNENEEDVEYPPIEPGEALEGHSRIDVSFNSEV NPTSPDSDHASPLPHPAPGNWPSYRETESASLDPQTKQGPPVGEVEVLDFKDPAELLDQTGTRTPAPPGA DAAFLTPGREDLGNRDTQSYPEARPVPSEPDVPVAPLEREILPNYPESGHVPPLSGGRYVVGLEDHVSSK DQVFTYNGANRETCEHSHGQCSRHAFCTDYTTGFCCHCQSRFYGNGRHCVPEGAPHRVNGKVSGHLQVGH MPVHFTDVDLHAYIVSNDGRAYTAISQIPQPAAQALLPVLPIGGLFGWLFALEKPGSENGFSLTGATFVH DVEVTFHPGEERVRITQTAEGLDPENYLSLNTNIEGQVPFIPANFTAHIAPYQEFYHHRDSVVTSSSSRS FSLISGSINQTRSYRIDQNITYKACSHAPRHLAVPATQQLTVDRAFALYSEEEGVLRFAVTNQIGPVEVD SAPTAVNPCYDGSHTCDTTARCHPGTGVDYTCECTPGFQGDGRSCVDVNECATGFHRCGPNSVCVNLVGS YRCECRSGYEFADDRHTCVLIAPPPNPCLDGSHTCAPEGQARCIHHGGSSFSCACLPGFVGTGHQCSDVD ECAENRCHGAAICYNTPGSFSCRCQPGYHGDGFHCASDTVPEDSISGLKPCEYQQRYAQAQHAHAGSRIH IPQCDDQGNFVPLQCHGSTGFCWCVDQNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERWRESLLEH YGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELPGTRSQPGTMPACIPTVAPPVVRPTPRP DVTPPAVGTFLLYAQGQQIGHLPLNGSRLQKDAARTLLSLHGSIVVGIDYDCRERMVYWTDVAGRTISRA SLEAGAEPETIITSGLISPEGLAIDHFRRTMYWTDSGLDKIERAGLDGSERKVLFHTDLVNPRAITVDPI RGNLYWTDWNREAPKIETSSLDGENRRILINKDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRR VIQNHLNYPFSVVSYADHFYHTDWRRDGVISVSKDSGQLTDEYLPEQRSHLYGITAVYPYCPTGRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001012005
ORF Size	4188 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001012005.2, NP_001012005.2
RefSeq Size	4782 bp
RefSeq ORF	4191 bp
Locus ID	302248
UniProt ID	B5DFC9
MW	153 kDa
Gene Summary	Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions (By similarity).[UniProtKB/Swiss-Prot Function]

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