Npc1l1 (NM_001002025) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	SLC66A2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204042 representing NM_001002025 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTGCCTGGCTGGGATGGCTGCTCTGGGCCCTGCTCCTGAGCGCGGCCCAGGGTGAGCTATACA CACCCAAACACGAAGCTGGGGTCTGCACCTTTTACGAAGAGTGCGGGAAAAACCCAGAGCTCTCTGGAGG CCTCACGTCACTATCCAATGTATCCTGCCTGTCTAACACCCCGGCCCGCCACGTCACGGGTGAACACCTG GCTCTTCTCCAGCGCATCTGTCCCCGCCTGTACAACGGCCCCAATACCACTTTTGCCTGTTGCTCTACCA AGCAGCTGCTGTCCTTAGAAAGCAGCATGTCCATCACCAAGGCCCTTCTCACGCGCTGCCCGGCCTGCTC TGACAATTTTGTGAGCTTACACTGCCACAACACTTGCAGCCCTGACCAGAGCCTCTTCATCAACGTCACC CGGGTGGTTGAGCGGGGCGCTGGAGAGCCTCCTGCCGTGGTGGCCTATGAGGCCTTTTATCAGCGCAGCT TTGCTGAGAAGGCCTATGAGTCCTGCAGCCAGGTGCGCATCCCTGCGGCCGCTTCCTTGGCCGTGGGCAG CATGTGTGGAGTGTATGGCTCCGCCCTCTGCAATGCTCAGCGCTGGCTCAACTTCCAAGGAGACACAGGG AATGGCCTGGCTCCGCTGGATATCACCTTCCACCTCTTGGAGCCTGGCCAGGCCCTACCGGATGGGATCC AGCCACTGAATGGGAAGATCGCACCCTGCAACGAGTCTCAGGGTGATGACTCAGCAGTCTGCTCCTGCCA GGACTGTGCGGCGTCCTGCCCTGTCATCCCTCCGCCCGAGGCCTTGCGCCCTTCCTTCTACATGGGTCGC ATGCCAGGCTGGCTGGCCCTCATCATCATCTTCACTGCTGTCTTTGTGTTGCTCTCTGCAGTCCTTGTGC GTCTCCGAGTGGTTTCCAACAGGAACAAGAACAAGGCAGAAGGCCCCCAGGAAGCCCCCAAACTCCCTCA TAAGCACAAACTCTCACCCCATACCATCCTGGGCCGGTTCTTCCAGAACTGGGGCACAAGGGTGGCCTCG TGGCCACTCACCGTCTTAGCACTGTCCTTCATCGTTGTGATAGCCTTAGCAGCAGGCCTGACCTTTATTG AACTCACCACAGACCCTGTGGAACTGTGGTCGGCCCCCAAGAGCCAGGCCCGGAAAGAGAAGTCTTTCCA TGATGAGCATTTCGGCCCCTTCTTTCGAACCAACCAGATTTTCGTGACAGCTCGGAACAGGTCCAGCTAC AAGTACGACTCCCTACTGCTAGGGTCCAAGAACTTCAGTGGGATCCTGTCCCTGGACTTCCTGCTGGAGC TGCTGGAGCTTCAGGAGAGGCTTCGACACCTGCAAGTGTGGTCCCCTGAGGCAGAGCGCAACATCTCCCT CCAGGACATCTGCTATGCCCCCCTCAACCCATATAACACCAGCCTCTCCGACTGCTGTGTCAACAGCCTC CTTCAGTACTTCCAGAACAACCGCACCCTCCTGATGCTCACGGCCAACCAGACTCTGAATGGCCAGACCT CCCTGGTGGACTGGAAGGACCATTTCCTCTACTGTGCAAATGCCCCTCTCACGTTCAAAGATGGCACGTC TCTGGCCCTGAGCTGCATGGCTGACTACGGGGCTCCTGTCTTCCCCTTCCTTGCTGTTGGGGGATACCAA GGCACGGACTATTCCGAGGCAGAAGCGCTGATCATAACCTTCTCTCTCAATAACTACCCCGCTGATGATC CCCGCATGGCCCAGGCCAAGCTCTGGGAGGAGGCTTTCTTGAAGGAAATGGAATCCTTCCAGAGGAACAC AAGTGACAAGTTCCAGGTTGCGTTCTCAGCTGAGCGCTCTCTGGAGGATGAGATCAACCGCACCACCATC CAGGACCTGCCTGTCTTTGCCGTCAGCTACATTATCGTCTTCCTGTACATCTCCCTGGCCCTGGGCAGCT ACTCCAGATGCAGCCGAGTAGCGGTGGAGTCCAAGGCTACTCTGGGCCTAGGTGGGGTGATTGTTGTGCT GGGAGCAGTTCTGGCTGCCATGGGCTTCTACTCCTACCTGGGTGTCCCCTCTTCTCTGGTTATCATCCAA GTGGTACCTTTCCTGGTGCTAGCTGTGGGAGCTGACAACATCTTCATCTTTGTTCTTGAGTACCAGAGGC TACCTAGGATGCCTGGGGAACAGCGAGAGGCTCACATTGGCCGCACCCTGGGCAGTGTGGCCCCCAGCAT GCTGCTGTGCAGCCTCTCTGAGGCCATCTGCTTCTTTCTAGGGGCCCTGACCCCCATGCCAGCTGTGAGG ACCTTCGCCTTGACCTCTGGCTTAGCAATTATCCTCGACTTCCTGCTCCAGATGACTGCCTTTGTGGCCC TGCTCTCCCTGGATAGCAAGAGGCAGGAGGCCTCTCGCCCGGATGTCTTATGCTGCTTTTCAACCCGGAA GCTGCCCCCACCTAAAGAAAAAGAAGGCCTCTTACTCCGCTTCTTCCGCAAGATATACGCTCCTTTCCTG CTGCACAGATTCATCCGCCCTGTTGTGATGCTGCTGTTTCTGACCCTGTTTGGAGCAAATCTCTACTTAA TGTGCAACATCAACGTGGGGCTAGACCAGGAGCTGGCTCTGCCCAAGGACTCGTACTTGATAGACTACTT CCTCTTTCTGAACCGATACCTTGAAGTGGGGCCTCCAGTGTACTTTGTCACCACCTCGGGCTTCAACTTC TCCAGCGAGGCAGGCATGAACGCCACTTGCTCTAGCGCAGGCTGTAAGAGCTTCTCCCTAACCCAGAAAA TCCAGTATGCCAGTGAATTCCCTGACCAGTCTTACGTGGCTATTGCTGCATCCTCCTGGGTAGATGACTT CATCGACTGGCTGACCCCGTCCTCCTCCTGCTGTCGCCTTTATATACGTGGCCCCCATAAGGATGAGTTC TGTCCCTCAACGGATACTTCCTTCAACTGCTTAAAAAACTGCATGAACCGCACTCTGGGTCCTGTGAGGC CCACAGCGGAACAGTTTCATAAGTACCTGCCCTGGTTCCTGAATGATCCGCCCAATATCAGATGTCCCAA AGGGGGTCTAGCAGCGTATAGAACGTCTGTGAATTTGAGCTCAGATGGCCAGGTTATAGCCTCCCAGTTC ATGGCCTACCACAAGCCCTTAAGGAACTCACAGGACTTCACAGAAGCTCTCCGGGCGTCCCGGTTGCTAG CAGCCAACATCACAGCTGACCTACGGAAGGTGCCTGGGACAGATCCAAACTTTGAGGTCTTCCCTTACAC GATCTCCAACGTGTTCTACCAGCAATACCTGACGGTCCTTCCTGAGGGAATCTTCACCCTTGCTCTTTGC TTTGTGCCCACCTTTGTTGTCTGCTACCTCCTACTGGGCCTGGACATGTGCTCAGGGATCCTCAACCTAC TCTCCATCATTATGATTCTCGTGGACACCATTGGCCTCATGGCTGTGTGGGGTATCAGCTATAATGCGGT ATCCCTCATCAACCTTGTCACGGCAGTGGGCATGTCTGTGGAGTTTGTGTCCCACATCACTCGGTCCTTT GCTGTAAGCACCAAGCCTACCCGGCTGGAGAGGGCTAAAGATGCTACTGTCTTCATGGGCAGTGCGGTGT TTGCTGGAGTGGCCATGACCAACTTCCCAGGCATCCTCATCTTGGGCTTTGCCCAAGCCCAGCTTATTCA GATCTTCTTCTTCCGCCTCAACCTTCTGATCACCTTGCTGGGTCTGCTGCATGGCCTGGTCTTCCTGCCG GTTGTCCTCAGCTATCTGGGACCAGATGTTAACCAAGCTCTGGTACAGGAGGAGAAACTAGCCAGCGAGG CAGCAGTGGCCCCAGAGCCTTCTTGCCCACAGTACCCCTCCCCTGCTGATGCGGATGCCAATGTTAACTA CGGCTTTGCCCCAGAACTTGCCCACGGAGCTAATGCTGCTAGAAGCTCTTTGCCCAAAAGTGACCAAAAG TTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001002025
Insert Size	3996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001002025.1, NP_001002025.1
RefSeq Size	4484 bp
RefSeq ORF	3996 bp
Locus ID	432367
UniProt ID	Q6T3U3
Gene Summary	mouse homolog plays a role in intestinal cholesterol absorption [RGD, Feb 2006]

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