Npc1l1 (NM_001002025) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	SLC66A2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204042 representing NM_001002025 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTGCCTGGCTGGGATGGCTGCTCTGGGCCCTGCTCCTGAGCGCGGCCCAGGGTGAGCTATACA CACCCAAACACGAAGCTGGGGTCTGCACCTTTTACGAAGAGTGCGGGAAAAACCCAGAGCTCTCTGGAGG CCTCACGTCACTATCCAATGTATCCTGCCTGTCTAACACCCCGGCCCGCCACGTCACGGGTGAACACCTG GCTCTTCTCCAGCGCATCTGTCCCCGCCTGTACAACGGCCCCAATACCACTTTTGCCTGTTGCTCTACCA AGCAGCTGCTGTCCTTAGAAAGCAGCATGTCCATCACCAAGGCCCTTCTCACGCGCTGCCCGGCCTGCTC TGACAATTTTGTGAGCTTACACTGCCACAACACTTGCAGCCCTGACCAGAGCCTCTTCATCAACGTCACC CGGGTGGTTGAGCGGGGCGCTGGAGAGCCTCCTGCCGTGGTGGCCTATGAGGCCTTTTATCAGCGCAGCT TTGCTGAGAAGGCCTATGAGTCCTGCAGCCAGGTGCGCATCCCTGCGGCCGCTTCCTTGGCCGTGGGCAG CATGTGTGGAGTGTATGGCTCCGCCCTCTGCAATGCTCAGCGCTGGCTCAACTTCCAAGGAGACACAGGG AATGGCCTGGCTCCGCTGGATATCACCTTCCACCTCTTGGAGCCTGGCCAGGCCCTACCGGATGGGATCC AGCCACTGAATGGGAAGATCGCACCCTGCAACGAGTCTCAGGGTGATGACTCAGCAGTCTGCTCCTGCCA GGACTGTGCGGCGTCCTGCCCTGTCATCCCTCCGCCCGAGGCCTTGCGCCCTTCCTTCTACATGGGTCGC ATGCCAGGCTGGCTGGCCCTCATCATCATCTTCACTGCTGTCTTTGTGTTGCTCTCTGCAGTCCTTGTGC GTCTCCGAGTGGTTTCCAACAGGAACAAGAACAAGGCAGAAGGCCCCCAGGAAGCCCCCAAACTCCCTCA TAAGCACAAACTCTCACCCCATACCATCCTGGGCCGGTTCTTCCAGAACTGGGGCACAAGGGTGGCCTCG TGGCCACTCACCGTCTTAGCACTGTCCTTCATCGTTGTGATAGCCTTAGCAGCAGGCCTGACCTTTATTG AACTCACCACAGACCCTGTGGAACTGTGGTCGGCCCCCAAGAGCCAGGCCCGGAAAGAGAAGTCTTTCCA TGATGAGCATTTCGGCCCCTTCTTTCGAACCAACCAGATTTTCGTGACAGCTCGGAACAGGTCCAGCTAC AAGTACGACTCCCTACTGCTAGGGTCCAAGAACTTCAGTGGGATCCTGTCCCTGGACTTCCTGCTGGAGC TGCTGGAGCTTCAGGAGAGGCTTCGACACCTGCAAGTGTGGTCCCCTGAGGCAGAGCGCAACATCTCCCT CCAGGACATCTGCTATGCCCCCCTCAACCCATATAACACCAGCCTCTCCGACTGCTGTGTCAACAGCCTC CTTCAGTACTTCCAGAACAACCGCACCCTCCTGATGCTCACGGCCAACCAGACTCTGAATGGCCAGACCT CCCTGGTGGACTGGAAGGACCATTTCCTCTACTGTGCAAATGCCCCTCTCACGTTCAAAGATGGCACGTC TCTGGCCCTGAGCTGCATGGCTGACTACGGGGCTCCTGTCTTCCCCTTCCTTGCTGTTGGGGGATACCAA GGCACGGACTATTCCGAGGCAGAAGCGCTGATCATAACCTTCTCTCTCAATAACTACCCCGCTGATGATC CCCGCATGGCCCAGGCCAAGCTCTGGGAGGAGGCTTTCTTGAAGGAAATGGAATCCTTCCAGAGGAACAC AAGTGACAAGTTCCAGGTTGCGTTCTCAGCTGAGCGCTCTCTGGAGGATGAGATCAACCGCACCACCATC CAGGACCTGCCTGTCTTTGCCGTCAGCTACATTATCGTCTTCCTGTACATCTCCCTGGCCCTGGGCAGCT ACTCCAGATGCAGCCGAGTAGCGGTGGAGTCCAAGGCTACTCTGGGCCTAGGTGGGGTGATTGTTGTGCT GGGAGCAGTTCTGGCTGCCATGGGCTTCTACTCCTACCTGGGTGTCCCCTCTTCTCTGGTTATCATCCAA GTGGTACCTTTCCTGGTGCTAGCTGTGGGAGCTGACAACATCTTCATCTTTGTTCTTGAGTACCAGAGGC TACCTAGGATGCCTGGGGAACAGCGAGAGGCTCACATTGGCCGCACCCTGGGCAGTGTGGCCCCCAGCAT GCTGCTGTGCAGCCTCTCTGAGGCCATCTGCTTCTTTCTAGGGGCCCTGACCCCCATGCCAGCTGTGAGG ACCTTCGCCTTGACCTCTGGCTTAGCAATTATCCTCGACTTCCTGCTCCAGATGACTGCCTTTGTGGCCC TGCTCTCCCTGGATAGCAAGAGGCAGGAGGCCTCTCGCCCGGATGTCTTATGCTGCTTTTCAACCCGGAA GCTGCCCCCACCTAAAGAAAAAGAAGGCCTCTTACTCCGCTTCTTCCGCAAGATATACGCTCCTTTCCTG CTGCACAGATTCATCCGCCCTGTTGTGATGCTGCTGTTTCTGACCCTGTTTGGAGCAAATCTCTACTTAA TGTGCAACATCAACGTGGGGCTAGACCAGGAGCTGGCTCTGCCCAAGGACTCGTACTTGATAGACTACTT CCTCTTTCTGAACCGATACCTTGAAGTGGGGCCTCCAGTGTACTTTGTCACCACCTCGGGCTTCAACTTC TCCAGCGAGGCAGGCATGAACGCCACTTGCTCTAGCGCAGGCTGTAAGAGCTTCTCCCTAACCCAGAAAA TCCAGTATGCCAGTGAATTCCCTGACCAGTCTTACGTGGCTATTGCTGCATCCTCCTGGGTAGATGACTT CATCGACTGGCTGACCCCGTCCTCCTCCTGCTGTCGCCTTTATATACGTGGCCCCCATAAGGATGAGTTC TGTCCCTCAACGGATACTTCCTTCAACTGCTTAAAAAACTGCATGAACCGCACTCTGGGTCCTGTGAGGC CCACAGCGGAACAGTTTCATAAGTACCTGCCCTGGTTCCTGAATGATCCGCCCAATATCAGATGTCCCAA AGGGGGTCTAGCAGCGTATAGAACGTCTGTGAATTTGAGCTCAGATGGCCAGGTTATAGCCTCCCAGTTC ATGGCCTACCACAAGCCCTTAAGGAACTCACAGGACTTCACAGAAGCTCTCCGGGCGTCCCGGTTGCTAG CAGCCAACATCACAGCTGACCTACGGAAGGTGCCTGGGACAGATCCAAACTTTGAGGTCTTCCCTTACAC GATCTCCAACGTGTTCTACCAGCAATACCTGACGGTCCTTCCTGAGGGAATCTTCACCCTTGCTCTTTGC TTTGTGCCCACCTTTGTTGTCTGCTACCTCCTACTGGGCCTGGACATGTGCTCAGGGATCCTCAACCTAC TCTCCATCATTATGATTCTCGTGGACACCATTGGCCTCATGGCTGTGTGGGGTATCAGCTATAATGCGGT ATCCCTCATCAACCTTGTCACGGCAGTGGGCATGTCTGTGGAGTTTGTGTCCCACATCACTCGGTCCTTT GCTGTAAGCACCAAGCCTACCCGGCTGGAGAGGGCTAAAGATGCTACTGTCTTCATGGGCAGTGCGGTGT TTGCTGGAGTGGCCATGACCAACTTCCCAGGCATCCTCATCTTGGGCTTTGCCCAAGCCCAGCTTATTCA GATCTTCTTCTTCCGCCTCAACCTTCTGATCACCTTGCTGGGTCTGCTGCATGGCCTGGTCTTCCTGCCG GTTGTCCTCAGCTATCTGGGACCAGATGTTAACCAAGCTCTGGTACAGGAGGAGAAACTAGCCAGCGAGG CAGCAGTGGCCCCAGAGCCTTCTTGCCCACAGTACCCCTCCCCTGCTGATGCGGATGCCAATGTTAACTA CGGCTTTGCCCCAGAACTTGCCCACGGAGCTAATGCTGCTAGAAGCTCTTTGCCCAAAAGTGACCAAAAG TTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204042 representing NM_001002025 Red=Cloning site Green=Tags(s) MAAAWLGWLLWALLLSAAQGELYTPKHEAGVCTFYEECGKNPELSGGLTSLSNVSCLSNTPARHVTGEHL ALLQRICPRLYNGPNTTFACCSTKQLLSLESSMSITKALLTRCPACSDNFVSLHCHNTCSPDQSLFINVT RVVERGAGEPPAVVAYEAFYQRSFAEKAYESCSQVRIPAAASLAVGSMCGVYGSALCNAQRWLNFQGDTG NGLAPLDITFHLLEPGQALPDGIQPLNGKIAPCNESQGDDSAVCSCQDCAASCPVIPPPEALRPSFYMGR MPGWLALIIIFTAVFVLLSAVLVRLRVVSNRNKNKAEGPQEAPKLPHKHKLSPHTILGRFFQNWGTRVAS WPLTVLALSFIVVIALAAGLTFIELTTDPVELWSAPKSQARKEKSFHDEHFGPFFRTNQIFVTARNRSSY KYDSLLLGSKNFSGILSLDFLLELLELQERLRHLQVWSPEAERNISLQDICYAPLNPYNTSLSDCCVNSL LQYFQNNRTLLMLTANQTLNGQTSLVDWKDHFLYCANAPLTFKDGTSLALSCMADYGAPVFPFLAVGGYQ GTDYSEAEALIITFSLNNYPADDPRMAQAKLWEEAFLKEMESFQRNTSDKFQVAFSAERSLEDEINRTTI QDLPVFAVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQ VVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTPMPAVR TFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKEKEGLLLRFFRKIYAPFL LHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPKDSYLIDYFLFLNRYLEVGPPVYFVTTSGFNF SSEAGMNATCSSAGCKSFSLTQKIQYASEFPDQSYVAIAASSWVDDFIDWLTPSSSCCRLYIRGPHKDEF CPSTDTSFNCLKNCMNRTLGPVRPTAEQFHKYLPWFLNDPPNIRCPKGGLAAYRTSVNLSSDGQVIASQF MAYHKPLRNSQDFTEALRASRLLAANITADLRKVPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALC FVPTFVVCYLLLGLDMCSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSF AVSTKPTRLERAKDATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLP VVLSYLGPDVNQALVQEEKLASEAAVAPEPSCPQYPSPADADANVNYGFAPELAHGANAARSSLPKSDQK F TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001002025
ORF Size	3993 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001002025.1, NP_001002025.1
RefSeq Size	4484 bp
RefSeq ORF	3996 bp
Locus ID	432367
UniProt ID	Q6T3U3
MW	146.4 kDa
Gene Summary	mouse homolog plays a role in intestinal cholesterol absorption [RGD, Feb 2006]

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