Npc1l1 (NM_001002025) Rat Tagged ORF Clone
CAT#: RR204042
- TrueORF®
Npc1l1 (Myc-DDK-tagged ORF) - Rat NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (Npc1l1), (10 ug)
"NM_001002025" in other vectors (1)
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CNY 21,470.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SLC66A2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204042 representing NM_001002025
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCTGCCTGGCTGGGATGGCTGCTCTGGGCCCTGCTCCTGAGCGCGGCCCAGGGTGAGCTATACA CACCCAAACACGAAGCTGGGGTCTGCACCTTTTACGAAGAGTGCGGGAAAAACCCAGAGCTCTCTGGAGG CCTCACGTCACTATCCAATGTATCCTGCCTGTCTAACACCCCGGCCCGCCACGTCACGGGTGAACACCTG GCTCTTCTCCAGCGCATCTGTCCCCGCCTGTACAACGGCCCCAATACCACTTTTGCCTGTTGCTCTACCA AGCAGCTGCTGTCCTTAGAAAGCAGCATGTCCATCACCAAGGCCCTTCTCACGCGCTGCCCGGCCTGCTC TGACAATTTTGTGAGCTTACACTGCCACAACACTTGCAGCCCTGACCAGAGCCTCTTCATCAACGTCACC CGGGTGGTTGAGCGGGGCGCTGGAGAGCCTCCTGCCGTGGTGGCCTATGAGGCCTTTTATCAGCGCAGCT TTGCTGAGAAGGCCTATGAGTCCTGCAGCCAGGTGCGCATCCCTGCGGCCGCTTCCTTGGCCGTGGGCAG CATGTGTGGAGTGTATGGCTCCGCCCTCTGCAATGCTCAGCGCTGGCTCAACTTCCAAGGAGACACAGGG AATGGCCTGGCTCCGCTGGATATCACCTTCCACCTCTTGGAGCCTGGCCAGGCCCTACCGGATGGGATCC AGCCACTGAATGGGAAGATCGCACCCTGCAACGAGTCTCAGGGTGATGACTCAGCAGTCTGCTCCTGCCA GGACTGTGCGGCGTCCTGCCCTGTCATCCCTCCGCCCGAGGCCTTGCGCCCTTCCTTCTACATGGGTCGC ATGCCAGGCTGGCTGGCCCTCATCATCATCTTCACTGCTGTCTTTGTGTTGCTCTCTGCAGTCCTTGTGC GTCTCCGAGTGGTTTCCAACAGGAACAAGAACAAGGCAGAAGGCCCCCAGGAAGCCCCCAAACTCCCTCA TAAGCACAAACTCTCACCCCATACCATCCTGGGCCGGTTCTTCCAGAACTGGGGCACAAGGGTGGCCTCG TGGCCACTCACCGTCTTAGCACTGTCCTTCATCGTTGTGATAGCCTTAGCAGCAGGCCTGACCTTTATTG AACTCACCACAGACCCTGTGGAACTGTGGTCGGCCCCCAAGAGCCAGGCCCGGAAAGAGAAGTCTTTCCA TGATGAGCATTTCGGCCCCTTCTTTCGAACCAACCAGATTTTCGTGACAGCTCGGAACAGGTCCAGCTAC AAGTACGACTCCCTACTGCTAGGGTCCAAGAACTTCAGTGGGATCCTGTCCCTGGACTTCCTGCTGGAGC TGCTGGAGCTTCAGGAGAGGCTTCGACACCTGCAAGTGTGGTCCCCTGAGGCAGAGCGCAACATCTCCCT CCAGGACATCTGCTATGCCCCCCTCAACCCATATAACACCAGCCTCTCCGACTGCTGTGTCAACAGCCTC CTTCAGTACTTCCAGAACAACCGCACCCTCCTGATGCTCACGGCCAACCAGACTCTGAATGGCCAGACCT CCCTGGTGGACTGGAAGGACCATTTCCTCTACTGTGCAAATGCCCCTCTCACGTTCAAAGATGGCACGTC TCTGGCCCTGAGCTGCATGGCTGACTACGGGGCTCCTGTCTTCCCCTTCCTTGCTGTTGGGGGATACCAA GGCACGGACTATTCCGAGGCAGAAGCGCTGATCATAACCTTCTCTCTCAATAACTACCCCGCTGATGATC CCCGCATGGCCCAGGCCAAGCTCTGGGAGGAGGCTTTCTTGAAGGAAATGGAATCCTTCCAGAGGAACAC AAGTGACAAGTTCCAGGTTGCGTTCTCAGCTGAGCGCTCTCTGGAGGATGAGATCAACCGCACCACCATC CAGGACCTGCCTGTCTTTGCCGTCAGCTACATTATCGTCTTCCTGTACATCTCCCTGGCCCTGGGCAGCT ACTCCAGATGCAGCCGAGTAGCGGTGGAGTCCAAGGCTACTCTGGGCCTAGGTGGGGTGATTGTTGTGCT GGGAGCAGTTCTGGCTGCCATGGGCTTCTACTCCTACCTGGGTGTCCCCTCTTCTCTGGTTATCATCCAA GTGGTACCTTTCCTGGTGCTAGCTGTGGGAGCTGACAACATCTTCATCTTTGTTCTTGAGTACCAGAGGC TACCTAGGATGCCTGGGGAACAGCGAGAGGCTCACATTGGCCGCACCCTGGGCAGTGTGGCCCCCAGCAT GCTGCTGTGCAGCCTCTCTGAGGCCATCTGCTTCTTTCTAGGGGCCCTGACCCCCATGCCAGCTGTGAGG ACCTTCGCCTTGACCTCTGGCTTAGCAATTATCCTCGACTTCCTGCTCCAGATGACTGCCTTTGTGGCCC TGCTCTCCCTGGATAGCAAGAGGCAGGAGGCCTCTCGCCCGGATGTCTTATGCTGCTTTTCAACCCGGAA GCTGCCCCCACCTAAAGAAAAAGAAGGCCTCTTACTCCGCTTCTTCCGCAAGATATACGCTCCTTTCCTG CTGCACAGATTCATCCGCCCTGTTGTGATGCTGCTGTTTCTGACCCTGTTTGGAGCAAATCTCTACTTAA TGTGCAACATCAACGTGGGGCTAGACCAGGAGCTGGCTCTGCCCAAGGACTCGTACTTGATAGACTACTT CCTCTTTCTGAACCGATACCTTGAAGTGGGGCCTCCAGTGTACTTTGTCACCACCTCGGGCTTCAACTTC TCCAGCGAGGCAGGCATGAACGCCACTTGCTCTAGCGCAGGCTGTAAGAGCTTCTCCCTAACCCAGAAAA TCCAGTATGCCAGTGAATTCCCTGACCAGTCTTACGTGGCTATTGCTGCATCCTCCTGGGTAGATGACTT CATCGACTGGCTGACCCCGTCCTCCTCCTGCTGTCGCCTTTATATACGTGGCCCCCATAAGGATGAGTTC TGTCCCTCAACGGATACTTCCTTCAACTGCTTAAAAAACTGCATGAACCGCACTCTGGGTCCTGTGAGGC CCACAGCGGAACAGTTTCATAAGTACCTGCCCTGGTTCCTGAATGATCCGCCCAATATCAGATGTCCCAA AGGGGGTCTAGCAGCGTATAGAACGTCTGTGAATTTGAGCTCAGATGGCCAGGTTATAGCCTCCCAGTTC ATGGCCTACCACAAGCCCTTAAGGAACTCACAGGACTTCACAGAAGCTCTCCGGGCGTCCCGGTTGCTAG CAGCCAACATCACAGCTGACCTACGGAAGGTGCCTGGGACAGATCCAAACTTTGAGGTCTTCCCTTACAC GATCTCCAACGTGTTCTACCAGCAATACCTGACGGTCCTTCCTGAGGGAATCTTCACCCTTGCTCTTTGC TTTGTGCCCACCTTTGTTGTCTGCTACCTCCTACTGGGCCTGGACATGTGCTCAGGGATCCTCAACCTAC TCTCCATCATTATGATTCTCGTGGACACCATTGGCCTCATGGCTGTGTGGGGTATCAGCTATAATGCGGT ATCCCTCATCAACCTTGTCACGGCAGTGGGCATGTCTGTGGAGTTTGTGTCCCACATCACTCGGTCCTTT GCTGTAAGCACCAAGCCTACCCGGCTGGAGAGGGCTAAAGATGCTACTGTCTTCATGGGCAGTGCGGTGT TTGCTGGAGTGGCCATGACCAACTTCCCAGGCATCCTCATCTTGGGCTTTGCCCAAGCCCAGCTTATTCA GATCTTCTTCTTCCGCCTCAACCTTCTGATCACCTTGCTGGGTCTGCTGCATGGCCTGGTCTTCCTGCCG GTTGTCCTCAGCTATCTGGGACCAGATGTTAACCAAGCTCTGGTACAGGAGGAGAAACTAGCCAGCGAGG CAGCAGTGGCCCCAGAGCCTTCTTGCCCACAGTACCCCTCCCCTGCTGATGCGGATGCCAATGTTAACTA CGGCTTTGCCCCAGAACTTGCCCACGGAGCTAATGCTGCTAGAAGCTCTTTGCCCAAAAGTGACCAAAAG TTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204042 representing NM_001002025
Red=Cloning site Green=Tags(s) MAAAWLGWLLWALLLSAAQGELYTPKHEAGVCTFYEECGKNPELSGGLTSLSNVSCLSNTPARHVTGEHL ALLQRICPRLYNGPNTTFACCSTKQLLSLESSMSITKALLTRCPACSDNFVSLHCHNTCSPDQSLFINVT RVVERGAGEPPAVVAYEAFYQRSFAEKAYESCSQVRIPAAASLAVGSMCGVYGSALCNAQRWLNFQGDTG NGLAPLDITFHLLEPGQALPDGIQPLNGKIAPCNESQGDDSAVCSCQDCAASCPVIPPPEALRPSFYMGR MPGWLALIIIFTAVFVLLSAVLVRLRVVSNRNKNKAEGPQEAPKLPHKHKLSPHTILGRFFQNWGTRVAS WPLTVLALSFIVVIALAAGLTFIELTTDPVELWSAPKSQARKEKSFHDEHFGPFFRTNQIFVTARNRSSY KYDSLLLGSKNFSGILSLDFLLELLELQERLRHLQVWSPEAERNISLQDICYAPLNPYNTSLSDCCVNSL LQYFQNNRTLLMLTANQTLNGQTSLVDWKDHFLYCANAPLTFKDGTSLALSCMADYGAPVFPFLAVGGYQ GTDYSEAEALIITFSLNNYPADDPRMAQAKLWEEAFLKEMESFQRNTSDKFQVAFSAERSLEDEINRTTI QDLPVFAVSYIIVFLYISLALGSYSRCSRVAVESKATLGLGGVIVVLGAVLAAMGFYSYLGVPSSLVIIQ VVPFLVLAVGADNIFIFVLEYQRLPRMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTPMPAVR TFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKEKEGLLLRFFRKIYAPFL LHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPKDSYLIDYFLFLNRYLEVGPPVYFVTTSGFNF SSEAGMNATCSSAGCKSFSLTQKIQYASEFPDQSYVAIAASSWVDDFIDWLTPSSSCCRLYIRGPHKDEF CPSTDTSFNCLKNCMNRTLGPVRPTAEQFHKYLPWFLNDPPNIRCPKGGLAAYRTSVNLSSDGQVIASQF MAYHKPLRNSQDFTEALRASRLLAANITADLRKVPGTDPNFEVFPYTISNVFYQQYLTVLPEGIFTLALC FVPTFVVCYLLLGLDMCSGILNLLSIIMILVDTIGLMAVWGISYNAVSLINLVTAVGMSVEFVSHITRSF AVSTKPTRLERAKDATVFMGSAVFAGVAMTNFPGILILGFAQAQLIQIFFFRLNLLITLLGLLHGLVFLP VVLSYLGPDVNQALVQEEKLASEAAVAPEPSCPQYPSPADADANVNYGFAPELAHGANAARSSLPKSDQK F TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001002025 |
ORF Size | 3993 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001002025.1, NP_001002025.1 |
RefSeq Size | 4484 bp |
RefSeq ORF | 3996 bp |
Locus ID | 432367 |
UniProt ID | Q6T3U3 |
MW | 146.4 kDa |
Gene Summary | mouse homolog plays a role in intestinal cholesterol absorption [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204042 | Npc1l1 (untagged ORF) - Rat NPC1 (Niemann-Pick disease, type C1, gene)-like 1 (Npc1l1), (10 ug) |
CNY 21,470.00 |