Polr2b (NM_001106002) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RpB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203199 representing NM_001106002 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACGCCGACGAGGATATGCAATATGATGAAGATGATGATGAAATCACTCCAGATTTGTGGCAGG AAGCATGCTGGATCGTGATCAGTTCTTATTTTGATGAAAAAGGCTTGGTCAGACAGCAGCTGGATTCCTT TGACGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGATGCACCACCGATTGACCTGCAGGCTGAA GCCCAGCACGCTAGTGGGGAGGTTGAAGAGCCGCCAAGATATTTACTGAAGTTTGAACAAATTTACCTCT CCAAACCTACTCATTGGGAAAGAGATGGTGCCCCTTCACCAATGATGCCAAATGAAGCCAGGTTAAGAAA TCTCACGTACTCCGCCCCGCTTTACGTGGACATAACGAAGACGGTCATTAAAGAGGGTGAAGAGCAGCTG CAGACTCAGCACCAGAAAACCTTCATCGGGAAAATCCCCATTATGCTGCGCTCCACGTATTGCTTGTTGA ATGGCTTAACAGACCGTGACTTGTGTGAGCTGAATGAGTGTCCTTTGGATCCTGGTGGCTATTTTATTAT TAATGGATCAGAAAAGGTTCTGATAGCTCAAGAGAAAATGGCAACAAACACAGTCTATGTGTTTGCCAAA AAGGATTCCAAGTATGCCTATACAGGAGAATGCCGCTCATGCCTAGAGAATTCTTCCCGACCCACCAGTA CTATATGGGTTAGCATGCTGGCCAGAGGAGGACAGGGTGCAAAGAAGAGTGCAATTGGCCAGCGCATCGT GGCGACATTACCATATATCAAGCAAGAAGTTCCCATCATAATTGTGTTCAGAGCATTAGGCTTTGTGTCT GATAGAGATATTTTAGAGCACATCATTTATGACTTCGAAGACCCAGAGATGATGGAAATGGTTAAACCAT CTCTCGATGAAGCATTTGTCATCCAAGAGCAGAATGTTGCCTTAAACTTCATTGGTTCAAGAGGGGCGAA GCCTGGTGTTACTAAGGAGAAAAGAATTAAATATGCAAAAGAAGTCTTACAGAAAGAGATGCTTCCGCAT GTCGGTGTCAGTGATTTCTGTGAGACCAAAAAGGCCTATTTCTTGGGGTACATGGTTCATAGGTTACTTC TAGCAGCCTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGCAACAAGAGATTGGATCTTGCTGG GCCTCTGCTTGCGTTCTTATTTAGAGGCATGTTTAAGAATTTGCTGAAAGAAGTACGGATCTATGCACAG AAGTTTATTGACCGCGGAAAGGACTTTAACCTAGAGTTGGCAATTAAGACAAGGATCATATCCGATGGGC TAAAATACTCTTTAGCCACTGGGAACTGGGGTGACCAGAAGAAAGCTCACCAAGCCAGAGCTGGAGTGTC GCAGGTACTGAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAACTCTCCTATTGGC AGAGATGGCAAGCTAGCAAAGCCGAGACAGTTGCATAATACATTGTGGGGGATGGTGTGTCCTGCTGAAA CTCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCCTGATGGCTTATATTTCAGTTGGATCTCA ACCTTCTCCAATTTTGGAATTTTTGGAAGAATGGAGCATGGAAAACTTAGAGGAAATTTCCCCTGCAGCT ATTGCCGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATCCATAAAGACCCGGAACAACTCA TGAACACACTCAGGAAATTACGGCGTCAGATGGACATCATTGTATCCGAAGTTTCTATGATCAGAGATAT CCGCGAGCGTGAGATTCGGATCTATACAGACGCAGGCCGTATTTGTAGACCACTTCTAATTGTGGAAAAG CAAAAGCTTCTTTTGAAGAAGAGACACATTGACCAGTTGAAAGAGAGAGAATATAACAACTACAGTTGGC AGGATCTGGTAGCCAGTGGGGTAGTAGAGTATATTGATACCCTGGAAGAAGAAACCGTGATGCTTGCAAT GACTCCAGATGATTTGCAGGAGAAAGAAGTAGCTTATTGCTCCACCTATACCCACTGTGAGATTCACCCA TCAATGATTCTTGGCGTCTGTGCATCTATTATTCCTTTTCCTGATCATAACCAGTCCCCTAGAAACACGT ACCAGTCTGCTATGGGCAAGCAGGCTATGGGAGTTTATATCACCAACTTCCACGTTCGGATGGACACCTT GGCGCACGTTCTCTACTATCCTCAGAAGCCCCTTGTGACTACACGGTCTATGGAGTATCTCCGGTTTAGA GAACTGCCAGCAGGAATCAACTCAATTGTGGCCATTGCATCATACACGGGCTATAATCAAGAAGACTCTG TTATCATGAATCGTTCAGCTGTGGATCGAGGCTTTTTCAGGTCTGTTTTCTACCGATCATACAAAGAACA AGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTGTTCGAGAAGCCTACACGTGAAACATGTCAGGGCATG AGGCATGCCATTTATGACAAGTTGGACGATGATGGCTTGATAGCGCCAGGGGTCCGAGTGTCGGGAGACG ATGTTATTATAGGCAAAACGGTCACCCTCCCTGAGAACGAGGATGAGCTGGAGAGCACCAACAGGCGCTA CACCAAGAGGGACTGCAGCACCTTCCTCAGAACCAGTGAGACAGGCATTGTGGACCAGGTCATGGTCACT CTCAACCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGAATTCCGCAGATCGGAGACA AATTTGCTAGCCGACATGGTCAGAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGACATGCCGTTCAC TTGTGAAGGCATAACTCCTGACATCATCATAAACCCCCACGCCATCCCCTCTCGGATGACGATTGGTCAC TTGATTGAATGTCTCCAAGGGAAGGTCTCTGCGAACAAGGGTGAAATTGGTGATGCTACTCCGTTTAATG ATGCTGTCAATGTGCAGAAGATTTCTAATCTTTTATCTGACTACGGCTACCATCTCAGAGGAAATGAGGT GCTCTACAATGGATTCACTGGTCGCAAAATCACATCGCAGATTTTTATTGGCCCCACTTACTACCAGCGT TTGAAGCATATGGTGGATGACAAAATTCATTCTCGTGCTCGGGGGCCCATTCAGATTCTTAACAGACAGC CCATGGAGGGCAGATCGCGTGATGGTGGCCTGCGCTTTGGAGAAATGGAGCGAGACTGTCAGATCGCACA TGGTGCAGCACAGTTCTTAAGAGAAAGGCTGTTTGAAGCATCAGATCCGTATCAGGTTCACGTTTGTAAT CTTTGTGGAATAATGGCAATTGCTAACACCAGGACTCACACATACGAATGCAGGGGATGCCGCAATAAGA CCCAGATCTCCTTGGTGCGAATGCCGTATGCGTGCAAGCTGTTGTTCCAGGAGCTCATGTCCATGAGCAT CGCACCACGGATGATGAGTGTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106002
Insert Size	3525 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106002.1, NP_001099472.1
RefSeq Size	3829 bp
RefSeq ORF	3525 bp
Locus ID	289561

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