Polr2b (NM_001106002) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RpB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203199 representing NM_001106002 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGACGCCGACGAGGATATGCAATATGATGAAGATGATGATGAAATCACTCCAGATTTGTGGCAGG AAGCATGCTGGATCGTGATCAGTTCTTATTTTGATGAAAAAGGCTTGGTCAGACAGCAGCTGGATTCCTT TGACGAGTTTATTCAGATGTCTGTTCAAAGAATTGTGGAAGATGCACCACCGATTGACCTGCAGGCTGAA GCCCAGCACGCTAGTGGGGAGGTTGAAGAGCCGCCAAGATATTTACTGAAGTTTGAACAAATTTACCTCT CCAAACCTACTCATTGGGAAAGAGATGGTGCCCCTTCACCAATGATGCCAAATGAAGCCAGGTTAAGAAA TCTCACGTACTCCGCCCCGCTTTACGTGGACATAACGAAGACGGTCATTAAAGAGGGTGAAGAGCAGCTG CAGACTCAGCACCAGAAAACCTTCATCGGGAAAATCCCCATTATGCTGCGCTCCACGTATTGCTTGTTGA ATGGCTTAACAGACCGTGACTTGTGTGAGCTGAATGAGTGTCCTTTGGATCCTGGTGGCTATTTTATTAT TAATGGATCAGAAAAGGTTCTGATAGCTCAAGAGAAAATGGCAACAAACACAGTCTATGTGTTTGCCAAA AAGGATTCCAAGTATGCCTATACAGGAGAATGCCGCTCATGCCTAGAGAATTCTTCCCGACCCACCAGTA CTATATGGGTTAGCATGCTGGCCAGAGGAGGACAGGGTGCAAAGAAGAGTGCAATTGGCCAGCGCATCGT GGCGACATTACCATATATCAAGCAAGAAGTTCCCATCATAATTGTGTTCAGAGCATTAGGCTTTGTGTCT GATAGAGATATTTTAGAGCACATCATTTATGACTTCGAAGACCCAGAGATGATGGAAATGGTTAAACCAT CTCTCGATGAAGCATTTGTCATCCAAGAGCAGAATGTTGCCTTAAACTTCATTGGTTCAAGAGGGGCGAA GCCTGGTGTTACTAAGGAGAAAAGAATTAAATATGCAAAAGAAGTCTTACAGAAAGAGATGCTTCCGCAT GTCGGTGTCAGTGATTTCTGTGAGACCAAAAAGGCCTATTTCTTGGGGTACATGGTTCATAGGTTACTTC TAGCAGCCTTGGGTAGAAGAGAACTAGATGACAGAGATCACTATGGCAACAAGAGATTGGATCTTGCTGG GCCTCTGCTTGCGTTCTTATTTAGAGGCATGTTTAAGAATTTGCTGAAAGAAGTACGGATCTATGCACAG AAGTTTATTGACCGCGGAAAGGACTTTAACCTAGAGTTGGCAATTAAGACAAGGATCATATCCGATGGGC TAAAATACTCTTTAGCCACTGGGAACTGGGGTGACCAGAAGAAAGCTCACCAAGCCAGAGCTGGAGTGTC GCAGGTACTGAACCGCCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAACTCTCCTATTGGC AGAGATGGCAAGCTAGCAAAGCCGAGACAGTTGCATAATACATTGTGGGGGATGGTGTGTCCTGCTGAAA CTCCAGAGGGCCATGCTGTAGGACTTGTGAAGAATTTAGCCCTGATGGCTTATATTTCAGTTGGATCTCA ACCTTCTCCAATTTTGGAATTTTTGGAAGAATGGAGCATGGAAAACTTAGAGGAAATTTCCCCTGCAGCT ATTGCCGATGCAACCAAGATTTTTGTTAATGGCTGCTGGGTTGGAATCCATAAAGACCCGGAACAACTCA TGAACACACTCAGGAAATTACGGCGTCAGATGGACATCATTGTATCCGAAGTTTCTATGATCAGAGATAT CCGCGAGCGTGAGATTCGGATCTATACAGACGCAGGCCGTATTTGTAGACCACTTCTAATTGTGGAAAAG CAAAAGCTTCTTTTGAAGAAGAGACACATTGACCAGTTGAAAGAGAGAGAATATAACAACTACAGTTGGC AGGATCTGGTAGCCAGTGGGGTAGTAGAGTATATTGATACCCTGGAAGAAGAAACCGTGATGCTTGCAAT GACTCCAGATGATTTGCAGGAGAAAGAAGTAGCTTATTGCTCCACCTATACCCACTGTGAGATTCACCCA TCAATGATTCTTGGCGTCTGTGCATCTATTATTCCTTTTCCTGATCATAACCAGTCCCCTAGAAACACGT ACCAGTCTGCTATGGGCAAGCAGGCTATGGGAGTTTATATCACCAACTTCCACGTTCGGATGGACACCTT GGCGCACGTTCTCTACTATCCTCAGAAGCCCCTTGTGACTACACGGTCTATGGAGTATCTCCGGTTTAGA GAACTGCCAGCAGGAATCAACTCAATTGTGGCCATTGCATCATACACGGGCTATAATCAAGAAGACTCTG TTATCATGAATCGTTCAGCTGTGGATCGAGGCTTTTTCAGGTCTGTTTTCTACCGATCATACAAAGAACA AGAGTCTAAAAAAGGATTTGATCAAGAAGAAGTGTTCGAGAAGCCTACACGTGAAACATGTCAGGGCATG AGGCATGCCATTTATGACAAGTTGGACGATGATGGCTTGATAGCGCCAGGGGTCCGAGTGTCGGGAGACG ATGTTATTATAGGCAAAACGGTCACCCTCCCTGAGAACGAGGATGAGCTGGAGAGCACCAACAGGCGCTA CACCAAGAGGGACTGCAGCACCTTCCTCAGAACCAGTGAGACAGGCATTGTGGACCAGGTCATGGTCACT CTCAACCAGGAAGGATATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGAATTCCGCAGATCGGAGACA AATTTGCTAGCCGACATGGTCAGAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGACATGCCGTTCAC TTGTGAAGGCATAACTCCTGACATCATCATAAACCCCCACGCCATCCCCTCTCGGATGACGATTGGTCAC TTGATTGAATGTCTCCAAGGGAAGGTCTCTGCGAACAAGGGTGAAATTGGTGATGCTACTCCGTTTAATG ATGCTGTCAATGTGCAGAAGATTTCTAATCTTTTATCTGACTACGGCTACCATCTCAGAGGAAATGAGGT GCTCTACAATGGATTCACTGGTCGCAAAATCACATCGCAGATTTTTATTGGCCCCACTTACTACCAGCGT TTGAAGCATATGGTGGATGACAAAATTCATTCTCGTGCTCGGGGGCCCATTCAGATTCTTAACAGACAGC CCATGGAGGGCAGATCGCGTGATGGTGGCCTGCGCTTTGGAGAAATGGAGCGAGACTGTCAGATCGCACA TGGTGCAGCACAGTTCTTAAGAGAAAGGCTGTTTGAAGCATCAGATCCGTATCAGGTTCACGTTTGTAAT CTTTGTGGAATAATGGCAATTGCTAACACCAGGACTCACACATACGAATGCAGGGGATGCCGCAATAAGA CCCAGATCTCCTTGGTGCGAATGCCGTATGCGTGCAAGCTGTTGTTCCAGGAGCTCATGTCCATGAGCAT CGCACCACGGATGATGAGTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203199 representing NM_001106002 Red=Cloning site Green=Tags(s) MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAE AQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQL QTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAK KDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVS DRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPH VGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIG RDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAA IADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEK QKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHP SMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFR ELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM RHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVT LNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGH LIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQR LKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCN LCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106002
ORF Size	3522 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106002.1, NP_001099472.1
RefSeq Size	3829 bp
RefSeq ORF	3525 bp
Locus ID	289561
MW	133.9 kDa

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