Col4a3 (NM_001135759) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202463 representing NM_001135759 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCAGAGACTGCTCCAAGGTTCTTGGCGCTCCTGCTGCCTATCCTGTTGCTGCTCCTGGCTGCCC CGCCTGTGGTTAGCAAGGGCTGTGTCTGCGAAGGCAAAGGAAAATGCCTCTGCTGGGGAACTAAAGGGGA GAAGGGGGAGATCGGCTTCCCTGGGCCCCCTGGATTTCCTGGCCAGAAAGGATTCCCAGGCCCTGAAGGC TTGCCTGGACCACAGGGACCCAAGGGCTCCCCAGGACTTCCAGGACTCACTGGCCCCAAAGGCATCAGGG GAATAACTGGATTACCAGGGTTTGCAGGTCCTCCTGGACTTCCAGGCATCCCAGGCTACCCTGGGCCTCC TGGGCTGGCTGGTTTACCAGGATGCAATGGCTCTAAGGGTGAACAAGGATTCCCAGGCATTCCTGGCACA CCAGGCTATGCAGGGCTCCCAGGTCCTGATGGCTTAAAAGGACAAAAGGGCAAGCCTGCTCAAGGAGAAG ACGGAGAATTCAATGGAAAAGGTGACCCTGGTCCTCCGGGGGCTCCAGGCTTCCAGGGTTTACCAGGACC CCCAGGTTTCCCAGGGCCTGCCGGCCCACCAGGGCCCCCAGGATTTTTTGGTTTCCCAGGAGCGATGGGA CCCAGAGGACCCAAAGGTCGCATGGGCGATAGCACGATAGGACAAGAAGGAGAAAAGGGTGTGAAAGGAT TAACAGGACCTCCTGGGCTGCCAGGACCAGTGATTTTTACACTCCGACACCCATACAGAAAATCGGACTT TCAGGGACAGAAAGGCGATGAGGGAGAGAGGGGTGAACCTGGACCTCCTGGACCCTCGGGACCACCTGGA GACTCCTATGGATCAGAAAAGGGTGCTCCTGGAGAGCCTGGTCCTCGGGGAAAACCTGGAAAAGACGGTG CTCCCGGCTTCCCTGGCACTGAGGGAGCCAAGGGAACCAGAGGCTTTCCTGGGTTGAGGGGTGAAGCCGG CATTAAGGGATGGAAAGGAGACATTGGCCCCCCAGGATTTCCTGGTCCAGTAAAATGTTATGATGCACAT CTGCAAAAGGGAGATAAAGGAATGCCAGGCCCGCCTGGGCCCAAAGGAGTCCGTGGCCCACGGGGTCCCA GTGGTCCCCCTGGAGTTCCTGGAAGTCCTGGACCGTCAAGGCCTGGCCTCAGAGGACCTGTCGGATGGCC AGGCTTGAAAGGGAGTAAAGGGGAGAGGGGACCCCCTGGGATAGACACCGTGGGCCCTCCTGGATCCCTG GGATGTCCTGGATCACCAGGTCCACCAGGCCCACCAGGACCTCCAGGACGTCCAGGTGACACTGTTTTTC AGCCAGGTCCACCTGGTGACCATGGAGCACCAGGCGATATAGGACCTCCAGGAGTTCCAGGAGTCGATGG ACCCAAAGGAGAACCAGGCCAACCTTGTACTGAGTGTCACTGCATCCCAGGGCCCCCAGGAGTCCCAGGA GTTCCAGGATTAGATGGTGTCAAAGGAATCCCAGGAGGACGAGGTGCGCCTGGTGTGAAGGGAAACCCAG GGTCCCCAGGAAATGCAGGTCTCCCAGGATTTGCAGGATTCCCAGGCGACCAAGGGCATCCAGGACTTAA AGGGGACAAAGGGGACACACCTCTACCCTGGGGACAAGTGGGCGATCCAGGTGATCCTGGACACAGAGGC CTGCCAGGGAGAAAGGGCTTCGATGGAAGTCCTGGAGGTCCAGGAGCAAAAGGGCCACGAGGACCTAGGG GCGAACCGGCTCTGAGTGGAAGGAAAGGGGACCAGGGACCTCCAGGAGCTCCTGGATCCCCTGGACCCCC AGGACCTGCAGGACCAGCTGGACCACCGGGCTATGGACCCCAAGGAGAGCCAGGTCCAAAGGGAGCCCAA GGAGTCCCTGGAGCCCTGGGACCACCAGGAGAAGCCGGTCTTAAAGGAGAATCCAGTGCATCAATACCAG TTCTAGGTCCCCCAGGCCCTCCAGGGCCCCCTGGTCAGGCTGGCCCCAGAGGTCTACCTGGTTTGCCTGG ACCTGTGGGAACATGTGATCCTGGTCATCCTGGACCTGATGGTGAACCAGGAATCCCAGAAGTTGGATTC CCAGGGGCCAGAGGACCTAAGGGAGATCAAGGTTTCCCAGGAACAATAGGATTACCTGGTTATCCTGGGG AAACGGGAAGACCTGGCTATCCCGGGGAGATGGGCGTCCCAGGAGCCAAGGGAGAACCATCAGTAGGCAG GCCTGGGGAGCCAGGAAAACCAGGCTTTCCAGGAGAAAGAGGCAATTCTGGGGAAAATGGAGATATTGGA CTCCCTGGACTTCCAGGCCCCCCTGGAACTCCAGGAAAAGACGGGTTTGACGGGCCTCCAGGAGACCCAG GGCAGTCTGGACCTCCAGGAGCAAAAGGACCCCCAGGGAGGTGCATACCAGGACCCAGGGGCACCCAAGG ACTTCCAGGTTTAAATGGATTGAAAGGACAACCAGGTAGAAGAGGTGATACCGGGCCAAAAGGAGACCCT GGCATCCCAGGGATGGACAGATCTGGAGTCCCTGGAGAGCGAGGACCACCAGGAACACCAGGTCTTCCGG GTGAGATGGGACCGCCTGGCCAAAAAGGATATCCAGGACCTCCAGGATTCCCAGGGTTACCAGGAGAGAA AGGAGAGGTTGGAATCATGGGCTATCCTGGAACCACTGGCCTTCCGGGGCTTCCTGGGAAACCAGGCTCA CAGGGGCAGAGGGGTAACCTCGGAATCCCAGGAGTAAAAGGGGAAAGAGGACGCCCGGGAGTCAAAGGCG AACGAGGCGAGAAGGGGAAACCTGGGCCTCCCCATGCACCACACTTAAAGGGGGACAAAGGAGAGCCTGG ACTTAAAGGGTTCGTAGGAAATCCAGGTGAAAAGGGAAACAGAGGCAATCCAGGGTTACCAGGCCCCAAA GGCCTCGAGGGAGTGCCTGGGCTACCAGGATCTCCAGGCCCTAGAGGAGATACGGGGAGCAGTGGGGATC CTGGAAGACCAGGACCACAAGGCTTGCCAGGAAGCATGGGGAACATGGGGGTGCCAGGCCCTAAAGGGAG AAAAGGAACTTCAGGATTTCCAGGTGTAGCTGGAAGACCAGGCCTGCCAGGGATCCCTGGCCCCCAGGGA GATAAGGGGGAGCCAGGTTATTCAGAAGGTGCAAGCCCAGGACCACCAGGACCGAAGGGTGATCCAGGAT TGCCAGGTGACAAAGGGAAGAAAGGAGAGAGAGGACTACCTGGGCCACCTGGACATTCGGGGCCTGCTGG ACCTGACGGAGCCCCTGGGAGTCCTGGGAGTCCTGGCCACCCAGGAAGACCAGGTCCTGATGGTGATTCC GGTCTTAAAGGACAGAAAGGTTTCCCCGGCCCTCCAGGAAGCACTGGCCCTCCAGGCCCTCCAGGACTCC CAGGACTTCCTGGACCAATGGGTATGAGAGGCGACCAAGGACAAGATGGAATTCCTGGTCCTCCGGGAGA AAAAGGAGAAACAGGCTTGCTGGGGGCCCATCCAGGCCAAAAAGGGAGCCCTGGTGTACCAGGTGTCAAA GGAGACAGGGGAGTTCCAGGCTTGTCTGGCCTTCCTGGCAGGAAGGGGACCATGGGGGATGTTGGACCTC AAGGACCCCCAGGCACCACTGGACTCCCAGGTCCACCAGGTCTGCCTGGGACCATTGTCCCTGGCCCAAA AGGAAACAGAGGTCTCCCCGGCTTACGAGGAAATCCAGGTGAGCCAGGTCCCCCTGGACCTCCAGGACCT GTCGGAGAAGGCATAAAGGGTGACAAAGGATTTATGGGCCTCCCTGGCTCCAGAGGTCTGCCTGGAATGG TAGGGGACACGGGTGCACCAGGTCAACCGGGAGCACCAGGCATCCCCGGTCTTCCCGGTGTCAGAGGTGA TCCAGGATTCCCCGGGTTTCCAGGTGTAAAAGGAGAGAAGGGTAATCCCGGATTTCTTGGGTCAATTGGA CATCCAGGACCAGTTGGGCCAAAAGGACCACCAGGTCCACAAGGAAAACCTGGCACACTTAAGGTCATCT CCCTTCCAGGAAGCCCAGGGCCTCCTGGTGCACCTGGACAGCCAGGAGTGAAAGGAGATCCTGGACCCCT GGGACCACCAGGAATTCCAGGGCCCTGTGGGCCAAGAGGTCAACCAGGCAAGGACGGAAAACCAGGAGCT CCAGGACCACCGGGAGTAAAGGGCAGCAAGGGCTCGAAAGGAGAACAAGGCCCACCTGGTCTGGATGGAT TGCCAGGTTTAAAGGGAAAACCTGGAGACAGAGGGACACCTGCCAACGGGACAAGAATGAGAGGCTTCAT CTTCACTCGACACAGTCAAACCACAGCCAATCCTTCATGCCCTGAAGGAACACAGCCACTCTACAGTGGG TTTTCCCTTCTTTTCGTACAAGGAAATGAGCACGCCCATGGACAAGACCTTGGTACTCTGGGCAGCTGCC TACAGCGATTCACCACAATGCCATTCTTATTCTGTAACGTCGATAACGTGTGTAACTTTGCATCACGAAA TGACTATTCATACTGGCTGTCGACACCAGCCCCGATGCCCATGGACATGGCCCCAATTACTGGCAGAGCT CTCGAACCCTACGTTAGCAGATGCACCGTCTGCGAAGGTCCTGCAATGGCTATAGCTGTTCACAGTCAAA CTACTGCTATCCCCCCATGTCCCCAGGGCTGGGTTTCTCTCTGGAAAGGTTTTTCCTTCGTTATGTTTAC AAGTGCAGGCTCTGAGGGTGCTGGACAAGCACTTGCCTCGCCCGGCTCCTGCCTGGAGGAATTCCGAGCC AGTCCGTTTATAGAATGCCATGGACGAGGGACCTGCAACTACTACTCAAACTCCTACAGCTTCTGGCTGG CTTCACTAAATCCAGAAAGAATGTTCAGAAAACCTATTCCATCAACTGTGAAAGCTGGAGACTTGGAGAA AATCATAAGCCGCTGTCAGGTGTGCATGAAGAAAAGACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135759
Insert Size	5013 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135759.1, NP_001129231.1
RefSeq Size	5020 bp
RefSeq ORF	5013 bp
Locus ID	363265
Gene Summary	autoimmunity to the NC1 domain of the human homolog causes the antiglomerular basement membrane (anti-GBM) disease [RGD, Feb 2006]

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