Col4a3 (NM_001135759) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202463 representing NM_001135759 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCAGAGACTGCTCCAAGGTTCTTGGCGCTCCTGCTGCCTATCCTGTTGCTGCTCCTGGCTGCCC CGCCTGTGGTTAGCAAGGGCTGTGTCTGCGAAGGCAAAGGAAAATGCCTCTGCTGGGGAACTAAAGGGGA GAAGGGGGAGATCGGCTTCCCTGGGCCCCCTGGATTTCCTGGCCAGAAAGGATTCCCAGGCCCTGAAGGC TTGCCTGGACCACAGGGACCCAAGGGCTCCCCAGGACTTCCAGGACTCACTGGCCCCAAAGGCATCAGGG GAATAACTGGATTACCAGGGTTTGCAGGTCCTCCTGGACTTCCAGGCATCCCAGGCTACCCTGGGCCTCC TGGGCTGGCTGGTTTACCAGGATGCAATGGCTCTAAGGGTGAACAAGGATTCCCAGGCATTCCTGGCACA CCAGGCTATGCAGGGCTCCCAGGTCCTGATGGCTTAAAAGGACAAAAGGGCAAGCCTGCTCAAGGAGAAG ACGGAGAATTCAATGGAAAAGGTGACCCTGGTCCTCCGGGGGCTCCAGGCTTCCAGGGTTTACCAGGACC CCCAGGTTTCCCAGGGCCTGCCGGCCCACCAGGGCCCCCAGGATTTTTTGGTTTCCCAGGAGCGATGGGA CCCAGAGGACCCAAAGGTCGCATGGGCGATAGCACGATAGGACAAGAAGGAGAAAAGGGTGTGAAAGGAT TAACAGGACCTCCTGGGCTGCCAGGACCAGTGATTTTTACACTCCGACACCCATACAGAAAATCGGACTT TCAGGGACAGAAAGGCGATGAGGGAGAGAGGGGTGAACCTGGACCTCCTGGACCCTCGGGACCACCTGGA GACTCCTATGGATCAGAAAAGGGTGCTCCTGGAGAGCCTGGTCCTCGGGGAAAACCTGGAAAAGACGGTG CTCCCGGCTTCCCTGGCACTGAGGGAGCCAAGGGAACCAGAGGCTTTCCTGGGTTGAGGGGTGAAGCCGG CATTAAGGGATGGAAAGGAGACATTGGCCCCCCAGGATTTCCTGGTCCAGTAAAATGTTATGATGCACAT CTGCAAAAGGGAGATAAAGGAATGCCAGGCCCGCCTGGGCCCAAAGGAGTCCGTGGCCCACGGGGTCCCA GTGGTCCCCCTGGAGTTCCTGGAAGTCCTGGACCGTCAAGGCCTGGCCTCAGAGGACCTGTCGGATGGCC AGGCTTGAAAGGGAGTAAAGGGGAGAGGGGACCCCCTGGGATAGACACCGTGGGCCCTCCTGGATCCCTG GGATGTCCTGGATCACCAGGTCCACCAGGCCCACCAGGACCTCCAGGACGTCCAGGTGACACTGTTTTTC AGCCAGGTCCACCTGGTGACCATGGAGCACCAGGCGATATAGGACCTCCAGGAGTTCCAGGAGTCGATGG ACCCAAAGGAGAACCAGGCCAACCTTGTACTGAGTGTCACTGCATCCCAGGGCCCCCAGGAGTCCCAGGA GTTCCAGGATTAGATGGTGTCAAAGGAATCCCAGGAGGACGAGGTGCGCCTGGTGTGAAGGGAAACCCAG GGTCCCCAGGAAATGCAGGTCTCCCAGGATTTGCAGGATTCCCAGGCGACCAAGGGCATCCAGGACTTAA AGGGGACAAAGGGGACACACCTCTACCCTGGGGACAAGTGGGCGATCCAGGTGATCCTGGACACAGAGGC CTGCCAGGGAGAAAGGGCTTCGATGGAAGTCCTGGAGGTCCAGGAGCAAAAGGGCCACGAGGACCTAGGG GCGAACCGGCTCTGAGTGGAAGGAAAGGGGACCAGGGACCTCCAGGAGCTCCTGGATCCCCTGGACCCCC AGGACCTGCAGGACCAGCTGGACCACCGGGCTATGGACCCCAAGGAGAGCCAGGTCCAAAGGGAGCCCAA GGAGTCCCTGGAGCCCTGGGACCACCAGGAGAAGCCGGTCTTAAAGGAGAATCCAGTGCATCAATACCAG TTCTAGGTCCCCCAGGCCCTCCAGGGCCCCCTGGTCAGGCTGGCCCCAGAGGTCTACCTGGTTTGCCTGG ACCTGTGGGAACATGTGATCCTGGTCATCCTGGACCTGATGGTGAACCAGGAATCCCAGAAGTTGGATTC CCAGGGGCCAGAGGACCTAAGGGAGATCAAGGTTTCCCAGGAACAATAGGATTACCTGGTTATCCTGGGG AAACGGGAAGACCTGGCTATCCCGGGGAGATGGGCGTCCCAGGAGCCAAGGGAGAACCATCAGTAGGCAG GCCTGGGGAGCCAGGAAAACCAGGCTTTCCAGGAGAAAGAGGCAATTCTGGGGAAAATGGAGATATTGGA CTCCCTGGACTTCCAGGCCCCCCTGGAACTCCAGGAAAAGACGGGTTTGACGGGCCTCCAGGAGACCCAG GGCAGTCTGGACCTCCAGGAGCAAAAGGACCCCCAGGGAGGTGCATACCAGGACCCAGGGGCACCCAAGG ACTTCCAGGTTTAAATGGATTGAAAGGACAACCAGGTAGAAGAGGTGATACCGGGCCAAAAGGAGACCCT GGCATCCCAGGGATGGACAGATCTGGAGTCCCTGGAGAGCGAGGACCACCAGGAACACCAGGTCTTCCGG GTGAGATGGGACCGCCTGGCCAAAAAGGATATCCAGGACCTCCAGGATTCCCAGGGTTACCAGGAGAGAA AGGAGAGGTTGGAATCATGGGCTATCCTGGAACCACTGGCCTTCCGGGGCTTCCTGGGAAACCAGGCTCA CAGGGGCAGAGGGGTAACCTCGGAATCCCAGGAGTAAAAGGGGAAAGAGGACGCCCGGGAGTCAAAGGCG AACGAGGCGAGAAGGGGAAACCTGGGCCTCCCCATGCACCACACTTAAAGGGGGACAAAGGAGAGCCTGG ACTTAAAGGGTTCGTAGGAAATCCAGGTGAAAAGGGAAACAGAGGCAATCCAGGGTTACCAGGCCCCAAA GGCCTCGAGGGAGTGCCTGGGCTACCAGGATCTCCAGGCCCTAGAGGAGATACGGGGAGCAGTGGGGATC CTGGAAGACCAGGACCACAAGGCTTGCCAGGAAGCATGGGGAACATGGGGGTGCCAGGCCCTAAAGGGAG AAAAGGAACTTCAGGATTTCCAGGTGTAGCTGGAAGACCAGGCCTGCCAGGGATCCCTGGCCCCCAGGGA GATAAGGGGGAGCCAGGTTATTCAGAAGGTGCAAGCCCAGGACCACCAGGACCGAAGGGTGATCCAGGAT TGCCAGGTGACAAAGGGAAGAAAGGAGAGAGAGGACTACCTGGGCCACCTGGACATTCGGGGCCTGCTGG ACCTGACGGAGCCCCTGGGAGTCCTGGGAGTCCTGGCCACCCAGGAAGACCAGGTCCTGATGGTGATTCC GGTCTTAAAGGACAGAAAGGTTTCCCCGGCCCTCCAGGAAGCACTGGCCCTCCAGGCCCTCCAGGACTCC CAGGACTTCCTGGACCAATGGGTATGAGAGGCGACCAAGGACAAGATGGAATTCCTGGTCCTCCGGGAGA AAAAGGAGAAACAGGCTTGCTGGGGGCCCATCCAGGCCAAAAAGGGAGCCCTGGTGTACCAGGTGTCAAA GGAGACAGGGGAGTTCCAGGCTTGTCTGGCCTTCCTGGCAGGAAGGGGACCATGGGGGATGTTGGACCTC AAGGACCCCCAGGCACCACTGGACTCCCAGGTCCACCAGGTCTGCCTGGGACCATTGTCCCTGGCCCAAA AGGAAACAGAGGTCTCCCCGGCTTACGAGGAAATCCAGGTGAGCCAGGTCCCCCTGGACCTCCAGGACCT GTCGGAGAAGGCATAAAGGGTGACAAAGGATTTATGGGCCTCCCTGGCTCCAGAGGTCTGCCTGGAATGG TAGGGGACACGGGTGCACCAGGTCAACCGGGAGCACCAGGCATCCCCGGTCTTCCCGGTGTCAGAGGTGA TCCAGGATTCCCCGGGTTTCCAGGTGTAAAAGGAGAGAAGGGTAATCCCGGATTTCTTGGGTCAATTGGA CATCCAGGACCAGTTGGGCCAAAAGGACCACCAGGTCCACAAGGAAAACCTGGCACACTTAAGGTCATCT CCCTTCCAGGAAGCCCAGGGCCTCCTGGTGCACCTGGACAGCCAGGAGTGAAAGGAGATCCTGGACCCCT GGGACCACCAGGAATTCCAGGGCCCTGTGGGCCAAGAGGTCAACCAGGCAAGGACGGAAAACCAGGAGCT CCAGGACCACCGGGAGTAAAGGGCAGCAAGGGCTCGAAAGGAGAACAAGGCCCACCTGGTCTGGATGGAT TGCCAGGTTTAAAGGGAAAACCTGGAGACAGAGGGACACCTGCCAACGGGACAAGAATGAGAGGCTTCAT CTTCACTCGACACAGTCAAACCACAGCCAATCCTTCATGCCCTGAAGGAACACAGCCACTCTACAGTGGG TTTTCCCTTCTTTTCGTACAAGGAAATGAGCACGCCCATGGACAAGACCTTGGTACTCTGGGCAGCTGCC TACAGCGATTCACCACAATGCCATTCTTATTCTGTAACGTCGATAACGTGTGTAACTTTGCATCACGAAA TGACTATTCATACTGGCTGTCGACACCAGCCCCGATGCCCATGGACATGGCCCCAATTACTGGCAGAGCT CTCGAACCCTACGTTAGCAGATGCACCGTCTGCGAAGGTCCTGCAATGGCTATAGCTGTTCACAGTCAAA CTACTGCTATCCCCCCATGTCCCCAGGGCTGGGTTTCTCTCTGGAAAGGTTTTTCCTTCGTTATGTTTAC AAGTGCAGGCTCTGAGGGTGCTGGACAAGCACTTGCCTCGCCCGGCTCCTGCCTGGAGGAATTCCGAGCC AGTCCGTTTATAGAATGCCATGGACGAGGGACCTGCAACTACTACTCAAACTCCTACAGCTTCTGGCTGG CTTCACTAAATCCAGAAAGAATGTTCAGAAAACCTATTCCATCAACTGTGAAAGCTGGAGACTTGGAGAA AATCATAAGCCGCTGTCAGGTGTGCATGAAGAAAAGACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202463 representing NM_001135759 Red=Cloning site Green=Tags(s) MHSETAPRFLALLLPILLLLLAAPPVVSKGCVCEGKGKCLCWGTKGEKGEIGFPGPPGFPGQKGFPGPEG LPGPQGPKGSPGLPGLTGPKGIRGITGLPGFAGPPGLPGIPGYPGPPGLAGLPGCNGSKGEQGFPGIPGT PGYAGLPGPDGLKGQKGKPAQGEDGEFNGKGDPGPPGAPGFQGLPGPPGFPGPAGPPGPPGFFGFPGAMG PRGPKGRMGDSTIGQEGEKGVKGLTGPPGLPGPVIFTLRHPYRKSDFQGQKGDEGERGEPGPPGPSGPPG DSYGSEKGAPGEPGPRGKPGKDGAPGFPGTEGAKGTRGFPGLRGEAGIKGWKGDIGPPGFPGPVKCYDAH LQKGDKGMPGPPGPKGVRGPRGPSGPPGVPGSPGPSRPGLRGPVGWPGLKGSKGERGPPGIDTVGPPGSL GCPGSPGPPGPPGPPGRPGDTVFQPGPPGDHGAPGDIGPPGVPGVDGPKGEPGQPCTECHCIPGPPGVPG VPGLDGVKGIPGGRGAPGVKGNPGSPGNAGLPGFAGFPGDQGHPGLKGDKGDTPLPWGQVGDPGDPGHRG LPGRKGFDGSPGGPGAKGPRGPRGEPALSGRKGDQGPPGAPGSPGPPGPAGPAGPPGYGPQGEPGPKGAQ GVPGALGPPGEAGLKGESSASIPVLGPPGPPGPPGQAGPRGLPGLPGPVGTCDPGHPGPDGEPGIPEVGF PGARGPKGDQGFPGTIGLPGYPGETGRPGYPGEMGVPGAKGEPSVGRPGEPGKPGFPGERGNSGENGDIG LPGLPGPPGTPGKDGFDGPPGDPGQSGPPGAKGPPGRCIPGPRGTQGLPGLNGLKGQPGRRGDTGPKGDP GIPGMDRSGVPGERGPPGTPGLPGEMGPPGQKGYPGPPGFPGLPGEKGEVGIMGYPGTTGLPGLPGKPGS QGQRGNLGIPGVKGERGRPGVKGERGEKGKPGPPHAPHLKGDKGEPGLKGFVGNPGEKGNRGNPGLPGPK GLEGVPGLPGSPGPRGDTGSSGDPGRPGPQGLPGSMGNMGVPGPKGRKGTSGFPGVAGRPGLPGIPGPQG DKGEPGYSEGASPGPPGPKGDPGLPGDKGKKGERGLPGPPGHSGPAGPDGAPGSPGSPGHPGRPGPDGDS GLKGQKGFPGPPGSTGPPGPPGLPGLPGPMGMRGDQGQDGIPGPPGEKGETGLLGAHPGQKGSPGVPGVK GDRGVPGLSGLPGRKGTMGDVGPQGPPGTTGLPGPPGLPGTIVPGPKGNRGLPGLRGNPGEPGPPGPPGP VGEGIKGDKGFMGLPGSRGLPGMVGDTGAPGQPGAPGIPGLPGVRGDPGFPGFPGVKGEKGNPGFLGSIG HPGPVGPKGPPGPQGKPGTLKVISLPGSPGPPGAPGQPGVKGDPGPLGPPGIPGPCGPRGQPGKDGKPGA PGPPGVKGSKGSKGEQGPPGLDGLPGLKGKPGDRGTPANGTRMRGFIFTRHSQTTANPSCPEGTQPLYSG FSLLFVQGNEHAHGQDLGTLGSCLQRFTTMPFLFCNVDNVCNFASRNDYSYWLSTPAPMPMDMAPITGRA LEPYVSRCTVCEGPAMAIAVHSQTTAIPPCPQGWVSLWKGFSFVMFTSAGSEGAGQALASPGSCLEEFRA SPFIECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGDLEKIISRCQVCMKKRH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001135759
ORF Size	5010 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001135759.1, NP_001129231.1
RefSeq Size	5020 bp
RefSeq ORF	5013 bp
Locus ID	363265
MW	161.6 kDa
Gene Summary	autoimmunity to the NC1 domain of the human homolog causes the antiglomerular basement membrane (anti-GBM) disease [RGD, Feb 2006]

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