Synj1 (NM_053476) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202059 representing NM_053476 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCAGCAAAGGATTTCGAATCTACCACAAATTGGATCCCCCACCGTTCAGCCTCATAGTGGAAA CTAGGCATAAGGAAGAATGTCTCATGTTCGAGTCTGGGGCCGTGGCAGTGCTCTCATCTGCAGAAAAGGA GGCCATTAAAGGCACATATGCCAAAGTGCTGGATGCATATGGACTTTTGGGAGTTTTACGATTAAATCTT GGTGATACCATGTTGCATTATCTGGTCCTAGTCACTGGATGCATGTCTGTTGGGAAAATTCAAGAATCTG AAGTCTTCCGAGTTACTTCCACTGAGTTTATATCACTTCGAGTTGATGCTTCAGATGAGGACCGCATTTC AGAAGTACGAAAGGTTTTGAACTCAGGAAACTTTTATTTTGCATGGTCTGCATCTGGAGTCAGCTTAGAT CTGAGTCTGAACGCACATCGAAGCATGCAGGAGCACACAACTGACAATAGGTTTTTCTGGAATCAGTCTT TGCATCTGCATCTCAAGCACTATGGTGTGAATTGTGATGACTGGTTGTTACGGCTCATGTGTGGAGGAGT AGAGATTAGAACAATTTATGCTGCCCATAAACAGGCAAAAGCTTGCCTCATTTCAAGACTAAGCTGTGAG CGAGCTGGGACAAGGTTCAACGTCCGGGGAACAAATGATGACGGTCATGTTGCCAACTTTGTAGAGACAG AGCAGGTTATATACTTAGATGACTGTGTCTCCTCCTTCATACAAATCCGAGGATCTGTTCCGTTGTTCTG GGAGCAACCAGGGTTGCAAGTGGGATCTCATCGTGTTCGAATGTCAAGGGGATTTGAAGCCAATGCGCCT GCCTTTGACAGGCATTTCCGAACCCTTAAGGACTTATATGGTAAACAAATAGTAGTAAATCTGCTTGGAT CTAAGGAGGGTGAGCATATGCTGAGCAAGGCCTTCCAGAGTCACTTGAAAGCCTCTGAACACGCTTCTGA TATCCACATGGTGTCCTTTGATTATCATCAAATGGTTAAAGGAGGAAAGGCAGAAAAATTGCACAGTGTT CTCAAACCACAAGTCCAGAAGTTTCTAGATTATGGATTTTTTTATTTCGATGGCAGTGCAGTTCAAAGGT GCCAGAGTGGTACAGTTCGGACAAACTGCCTGGACTGTCTGGATAGAACAAACAGTGTGCAGGCGTTCCT TGGCTTAGAGATGCTAGCTAAACAGCTGGAAGCTCTGGGCTTGGCTGAAAAGCCTCAGTTGGTGACTCGC TTTCAAGAAGTTTTCCGGTCCATGTGGTCTGTGAATGGCGATTCAATCAGTAAAATATATGCAGGCACCG GAGCCCTGGAAGGGAAGGCCAAGTTAAAAGATGGTGCGCGATCTGTCACCAGAACCATTCAGAATAACTT CTTCGACAGCTCCAAGCAAGAAGCCATTGATGTTCTGCTTCTGGGAAACACTCTGAACAGCGACTTAGCC GACAAAGCCCGGGCACTTCTAACTACTGGGAGTTTGCGTGTGTCTGAACAGACATTACAGTCAGCATCTT CCAAAGTCTTAAAGAACATGTGTGAGAACTTCTACAAGTACTCAAAGCCCAAGAAGATCCGAGTGTGTGT TGGAACCTGGAATGTGAACGGCGGGAAACAGTTCCGCAGCATAGCATTCAAGAACCAGACCCTCACCGAC TGGCTTCTTGATGCACCCAAGTTAGCTGGCATCCAGGAGTTTCAAGATAAGAGAAGTAAGCCAACTGATA TATTTGCAATTGGCTTTGAAGAAATGGTGGAGCTGAATGCTGGGAACATTGTGAATGCAAGCACAACAAA CCAGAAGCTGTGGGCAGTGGAACTGCAGAAGACCATCTCCAGAGACAACAAGTATGTTCTCCTGGCCTCG GAGCAGTTGGTGGGTGTCTGCCTGTTTGTCTTTATCAGACCGCAGCATGCTCCCTTTATCAGGGATGTTG CAGTCGATACTGTGAAAACTGGGATGGGAGGCGCGACTGGAAATAAGGGCGCTGTTGCAATCCGGATGCT CTTCCACACCACCAGCCTTTGCTTTGTCTGCAGTCACTTTGCTGCAGGCCAGTCCCAAGTCAAAGAGAGA AATGAAGATTTCGTAGAAATAGCACGAAAATTGAGTTTCCCCATGGGAAGAATGCTCTTCTCCCATGATT ACGTATTCTGGTGTGGTGATTTCAACTATCGAATCGATCTTCCTAATGAAGAAGTTAAAGAGCTCATAAG ACAGCAAAATTGGGATTCTCTTATAGCTGGAGATCAACTCATCAATCAGAAAAATGCAGGACAGATCTTT AGAGGATTTTTAGAGGGAAAAGTAACGTTTGCTCCAACCTATAAATATGACTTGTTTTCCGAAGACTATG ACACCAGTGAAAAGTGCCGCACCCCTGCTTGGACAGACCGAGTCCTCTGGAGAAGGAGGAAGTGGCCTTT TGACAGATCAGCTGAAGACTTAGATCTTCTGAATGCTAGTTTCCAAGATGAAAGTAAAATCCTGTATACA TGGACTCCTGGCACCTTGCTGCACTATGGAAGAGCCGAGCTGAAGACTTCTGACCACAGGCCCGTTGTCG CCCTGATCGATATAGATATATTTGAAGTTGAAGCTGAAGAGAGACAAAAAATTTATAAAGAAGTTATTGC AGTCCAGGGCCCACCGGACGGCACAGTACTGGTCTCAATCAAAAGTTCTGCACAAGAGAATACTTTCTTT GACGATGCTTTAATCGATGAGCTTCTGCAGCAGTTTGCACACTTCGGCGAAGTTATACTTATAAGATTCG TAGAAGATAAAATGTGGGTTACATTTTTGGAGGGGAGCTCTGCCTTGAATGTTCTGAGCCTAAATGGTAA AGAGTTATTAAATCGGACTATAACAATTACTTTAAAAAGTCCAGACTGGATCAAAACTTTGGAAGAAGAG ATGAGTTTAGAGAAAATCAGTGTCACATTGCCCTCATCAACCAGTTCCACCCTGCTCGGTGAAGATGCAG AGGTCTCAGCGGACTTTGACATGGAAGGTGATGTCGACGACTACAGTGCTGAGGTGGAGGAGCTTCTTCC TCAGCACCTCCAGCCATCCTCCAGCTCTGGCCTCGGCACCTCTCCAAGCTCTTCGCCCCGGACCAGTCCC TGCCAGTCACCCACAGCACCAGAGTACTCCGCACCTTCTCTTCCCATCAGACCTAGTCGAGCACCATCAA GAACTCCAGGACCTCTAAGTTCACAGGGTGCTCCAGTTGACACCCAGCCAGCAGCCCAGAAGGAGTCTTC CCAGACCATAGAGCCCAAGAGGCCACCCCCTCCCCGCCCAGTTGCTCCTCCAGCACGTCCTGCCCCACCA CAGAGGCCACCTCCACCTTCAGGGGCTAGGAGTCCGGCACCTGCTAGAAAAGAATTTGGAGCACCCAAAA GCCCGGGAACAGCACGGAAAGATAATATAGGGCGTAATCAGCCTTCCCCTCAAGCCGGACTTGCAGGCCC AGGACCTTCTGGATATGGTGCAGCTAGACCGACAATTCCAGCTCGTGCTGGAGTGATCAGTGCCCCTCAG AGCCAGGCTCGGGTGTCTGCTGGAAGGCTGACTCCTGAAAGCCAAAGCAAACCCTTGGAGACATCGAAAG GTCCTGCTGTCCTTCCAGAACCACTGAAGCCTCAGGCTGCATTTCCTCCGCAGCCTTCTCTGCCCACACC TGCTCAAAAGTTACAGGACCCACTTGTCCCCATAGCAGCACCTATGCCTCCCTCCATTCCCCAATCGAAT TTGGAAACTCCACCCCTGCCCCCACCTCGGAGCAGGTCATCTCAAAGCTTGCCTTCAGACTCCTCACCAC AGCTACAACAGGAGCAACCAACAGGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053476
Insert Size	3879 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053476.2, NP_445928.2
RefSeq Size	5970 bp
RefSeq ORF	3879 bp
Locus ID	85238
UniProt ID	Q62910
Gene Summary	has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.