Synj1 (NM_053476) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202059 representing NM_053476 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCAGCAAAGGATTTCGAATCTACCACAAATTGGATCCCCCACCGTTCAGCCTCATAGTGGAAA CTAGGCATAAGGAAGAATGTCTCATGTTCGAGTCTGGGGCCGTGGCAGTGCTCTCATCTGCAGAAAAGGA GGCCATTAAAGGCACATATGCCAAAGTGCTGGATGCATATGGACTTTTGGGAGTTTTACGATTAAATCTT GGTGATACCATGTTGCATTATCTGGTCCTAGTCACTGGATGCATGTCTGTTGGGAAAATTCAAGAATCTG AAGTCTTCCGAGTTACTTCCACTGAGTTTATATCACTTCGAGTTGATGCTTCAGATGAGGACCGCATTTC AGAAGTACGAAAGGTTTTGAACTCAGGAAACTTTTATTTTGCATGGTCTGCATCTGGAGTCAGCTTAGAT CTGAGTCTGAACGCACATCGAAGCATGCAGGAGCACACAACTGACAATAGGTTTTTCTGGAATCAGTCTT TGCATCTGCATCTCAAGCACTATGGTGTGAATTGTGATGACTGGTTGTTACGGCTCATGTGTGGAGGAGT AGAGATTAGAACAATTTATGCTGCCCATAAACAGGCAAAAGCTTGCCTCATTTCAAGACTAAGCTGTGAG CGAGCTGGGACAAGGTTCAACGTCCGGGGAACAAATGATGACGGTCATGTTGCCAACTTTGTAGAGACAG AGCAGGTTATATACTTAGATGACTGTGTCTCCTCCTTCATACAAATCCGAGGATCTGTTCCGTTGTTCTG GGAGCAACCAGGGTTGCAAGTGGGATCTCATCGTGTTCGAATGTCAAGGGGATTTGAAGCCAATGCGCCT GCCTTTGACAGGCATTTCCGAACCCTTAAGGACTTATATGGTAAACAAATAGTAGTAAATCTGCTTGGAT CTAAGGAGGGTGAGCATATGCTGAGCAAGGCCTTCCAGAGTCACTTGAAAGCCTCTGAACACGCTTCTGA TATCCACATGGTGTCCTTTGATTATCATCAAATGGTTAAAGGAGGAAAGGCAGAAAAATTGCACAGTGTT CTCAAACCACAAGTCCAGAAGTTTCTAGATTATGGATTTTTTTATTTCGATGGCAGTGCAGTTCAAAGGT GCCAGAGTGGTACAGTTCGGACAAACTGCCTGGACTGTCTGGATAGAACAAACAGTGTGCAGGCGTTCCT TGGCTTAGAGATGCTAGCTAAACAGCTGGAAGCTCTGGGCTTGGCTGAAAAGCCTCAGTTGGTGACTCGC TTTCAAGAAGTTTTCCGGTCCATGTGGTCTGTGAATGGCGATTCAATCAGTAAAATATATGCAGGCACCG GAGCCCTGGAAGGGAAGGCCAAGTTAAAAGATGGTGCGCGATCTGTCACCAGAACCATTCAGAATAACTT CTTCGACAGCTCCAAGCAAGAAGCCATTGATGTTCTGCTTCTGGGAAACACTCTGAACAGCGACTTAGCC GACAAAGCCCGGGCACTTCTAACTACTGGGAGTTTGCGTGTGTCTGAACAGACATTACAGTCAGCATCTT CCAAAGTCTTAAAGAACATGTGTGAGAACTTCTACAAGTACTCAAAGCCCAAGAAGATCCGAGTGTGTGT TGGAACCTGGAATGTGAACGGCGGGAAACAGTTCCGCAGCATAGCATTCAAGAACCAGACCCTCACCGAC TGGCTTCTTGATGCACCCAAGTTAGCTGGCATCCAGGAGTTTCAAGATAAGAGAAGTAAGCCAACTGATA TATTTGCAATTGGCTTTGAAGAAATGGTGGAGCTGAATGCTGGGAACATTGTGAATGCAAGCACAACAAA CCAGAAGCTGTGGGCAGTGGAACTGCAGAAGACCATCTCCAGAGACAACAAGTATGTTCTCCTGGCCTCG GAGCAGTTGGTGGGTGTCTGCCTGTTTGTCTTTATCAGACCGCAGCATGCTCCCTTTATCAGGGATGTTG CAGTCGATACTGTGAAAACTGGGATGGGAGGCGCGACTGGAAATAAGGGCGCTGTTGCAATCCGGATGCT CTTCCACACCACCAGCCTTTGCTTTGTCTGCAGTCACTTTGCTGCAGGCCAGTCCCAAGTCAAAGAGAGA AATGAAGATTTCGTAGAAATAGCACGAAAATTGAGTTTCCCCATGGGAAGAATGCTCTTCTCCCATGATT ACGTATTCTGGTGTGGTGATTTCAACTATCGAATCGATCTTCCTAATGAAGAAGTTAAAGAGCTCATAAG ACAGCAAAATTGGGATTCTCTTATAGCTGGAGATCAACTCATCAATCAGAAAAATGCAGGACAGATCTTT AGAGGATTTTTAGAGGGAAAAGTAACGTTTGCTCCAACCTATAAATATGACTTGTTTTCCGAAGACTATG ACACCAGTGAAAAGTGCCGCACCCCTGCTTGGACAGACCGAGTCCTCTGGAGAAGGAGGAAGTGGCCTTT TGACAGATCAGCTGAAGACTTAGATCTTCTGAATGCTAGTTTCCAAGATGAAAGTAAAATCCTGTATACA TGGACTCCTGGCACCTTGCTGCACTATGGAAGAGCCGAGCTGAAGACTTCTGACCACAGGCCCGTTGTCG CCCTGATCGATATAGATATATTTGAAGTTGAAGCTGAAGAGAGACAAAAAATTTATAAAGAAGTTATTGC AGTCCAGGGCCCACCGGACGGCACAGTACTGGTCTCAATCAAAAGTTCTGCACAAGAGAATACTTTCTTT GACGATGCTTTAATCGATGAGCTTCTGCAGCAGTTTGCACACTTCGGCGAAGTTATACTTATAAGATTCG TAGAAGATAAAATGTGGGTTACATTTTTGGAGGGGAGCTCTGCCTTGAATGTTCTGAGCCTAAATGGTAA AGAGTTATTAAATCGGACTATAACAATTACTTTAAAAAGTCCAGACTGGATCAAAACTTTGGAAGAAGAG ATGAGTTTAGAGAAAATCAGTGTCACATTGCCCTCATCAACCAGTTCCACCCTGCTCGGTGAAGATGCAG AGGTCTCAGCGGACTTTGACATGGAAGGTGATGTCGACGACTACAGTGCTGAGGTGGAGGAGCTTCTTCC TCAGCACCTCCAGCCATCCTCCAGCTCTGGCCTCGGCACCTCTCCAAGCTCTTCGCCCCGGACCAGTCCC TGCCAGTCACCCACAGCACCAGAGTACTCCGCACCTTCTCTTCCCATCAGACCTAGTCGAGCACCATCAA GAACTCCAGGACCTCTAAGTTCACAGGGTGCTCCAGTTGACACCCAGCCAGCAGCCCAGAAGGAGTCTTC CCAGACCATAGAGCCCAAGAGGCCACCCCCTCCCCGCCCAGTTGCTCCTCCAGCACGTCCTGCCCCACCA CAGAGGCCACCTCCACCTTCAGGGGCTAGGAGTCCGGCACCTGCTAGAAAAGAATTTGGAGCACCCAAAA GCCCGGGAACAGCACGGAAAGATAATATAGGGCGTAATCAGCCTTCCCCTCAAGCCGGACTTGCAGGCCC AGGACCTTCTGGATATGGTGCAGCTAGACCGACAATTCCAGCTCGTGCTGGAGTGATCAGTGCCCCTCAG AGCCAGGCTCGGGTGTCTGCTGGAAGGCTGACTCCTGAAAGCCAAAGCAAACCCTTGGAGACATCGAAAG GTCCTGCTGTCCTTCCAGAACCACTGAAGCCTCAGGCTGCATTTCCTCCGCAGCCTTCTCTGCCCACACC TGCTCAAAAGTTACAGGACCCACTTGTCCCCATAGCAGCACCTATGCCTCCCTCCATTCCCCAATCGAAT TTGGAAACTCCACCCCTGCCCCCACCTCGGAGCAGGTCATCTCAAAGCTTGCCTTCAGACTCCTCACCAC AGCTACAACAGGAGCAACCAACAGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202059 representing NM_053476 Red=Cloning site Green=Tags(s) MAFSKGFRIYHKLDPPPFSLIVETRHKEECLMFESGAVAVLSSAEKEAIKGTYAKVLDAYGLLGVLRLNL GDTMLHYLVLVTGCMSVGKIQESEVFRVTSTEFISLRVDASDEDRISEVRKVLNSGNFYFAWSASGVSLD LSLNAHRSMQEHTTDNRFFWNQSLHLHLKHYGVNCDDWLLRLMCGGVEIRTIYAAHKQAKACLISRLSCE RAGTRFNVRGTNDDGHVANFVETEQVIYLDDCVSSFIQIRGSVPLFWEQPGLQVGSHRVRMSRGFEANAP AFDRHFRTLKDLYGKQIVVNLLGSKEGEHMLSKAFQSHLKASEHASDIHMVSFDYHQMVKGGKAEKLHSV LKPQVQKFLDYGFFYFDGSAVQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQLVTR FQEVFRSMWSVNGDSISKIYAGTGALEGKAKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLA DKARALLTTGSLRVSEQTLQSASSKVLKNMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTD WLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRDNKYVLLAS EQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCSHFAAGQSQVKER NEDFVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIF RGFLEGKVTFAPTYKYDLFSEDYDTSEKCRTPAWTDRVLWRRRKWPFDRSAEDLDLLNASFQDESKILYT WTPGTLLHYGRAELKTSDHRPVVALIDIDIFEVEAEERQKIYKEVIAVQGPPDGTVLVSIKSSAQENTFF DDALIDELLQQFAHFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITITLKSPDWIKTLEEE MSLEKISVTLPSSTSSTLLGEDAEVSADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSP CQSPTAPEYSAPSLPIRPSRAPSRTPGPLSSQGAPVDTQPAAQKESSQTIEPKRPPPPRPVAPPARPAPP QRPPPPSGARSPAPARKEFGAPKSPGTARKDNIGRNQPSPQAGLAGPGPSGYGAARPTIPARAGVISAPQ SQARVSAGRLTPESQSKPLETSKGPAVLPEPLKPQAAFPPQPSLPTPAQKLQDPLVPIAAPMPPSIPQSN LETPPLPPPRSRSSQSLPSDSSPQLQQEQPTG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053476
ORF Size	3876 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053476.2, NP_445928.2
RefSeq Size	5970 bp
RefSeq ORF	3879 bp
Locus ID	85238
UniProt ID	Q62910
MW	142.9 kDa
Gene Summary	has inositol 5-phosphatase activity; interacts with dynamin to play a role in synaptic vesicle recycling [RGD, Feb 2006]

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