Akap12 (NM_001033653) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AKAP12A; AKAP12B; AKAP12G
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200267 representing NM_001033653 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGACCATTACCATCACAGTTGGACAGCGAGAGTCAGAAGATGTGAGAGAAAAAGACCGAGTTG AAGAAATGGCGGCCAACTCCACAGCTGTTGAAGATATCACAAAGGATGGGCAGGAGGAGACATCAGAAAT AATTGAACAGATCCCTGCTTCAGAAAGCAATGTGGAAGAAATGGTACAGCCTGCTGAGTCCCAGGCTAAT GATGTTGGCTTCAAGAAAGTATTTAAATTTGTTGGTTTTAAATTCACGGTGAAGAAGGATAAAAATGAAA AGTCAGATACTGTCCAACTACTCACTGTCAAGAAGGATGAAGGCGAAGGGGCAGAAGCCTCTGTCGGAGC TGGAGACCACCAGGAGCCCAGTGTGGAGACTGCCGTCGGAGAGTCAGCATCCAAAGAAAGTGAGCTGAAG CAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGAACAGAGCAGCACAGAAATCCCCCTTCAAGCCG AATCTGATCAAGCGGCTGAGGAAGAAGCCAAAGATGAAGGAGAAGAAAAACAAGAGAAAGAGCCCACCAA GTCCCCAGAATCCCCGAGCAGCCCAGTCAGCAGTGAGACAACATCTTCCTTCAAGAAGTTCTTCACTCAC GGTTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAATCAAAAGAGGATGATCTGGAAACTGCCGAGA AGAGAAAGGAGCAAGAGGCAGAAAAAGTAGACGAGGAAGAAAAGGAAAAGACAGAGCCAGCCTCGGAGGA GCAGGAGCCGGCAGAAGACACAGACCAGGCCAGGTTGTCAGCAGACTACGAGAAGGTGGAGCTGCCTTTG GAAGACCAGGTTGGTGACCTGGAGGCATCGTCAGAGGAGAAGTGTGCTCCTTTGGCAACGGAAGTGTTTG ATGAGAAGATGGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCACGTGAGCACCGTGGAGAAGACAGAGGA GGAGCAGGGAGGAGGAGGAGAGGCTGAAGGGGGCGTGGTGGTAGAAGGAACAGGAGAATCCTTGCCCCCT GAGAAACTGGCTGAGCCCCAGGAGGTCCCCCAGGAAGCTGAGCCTGCTGAGGAGCTGATGAAGAGCAGAG AGATGTGTGTCTCTGGAGGAGACCACACTCAACTGACAGACCTAAGTCCTGAAGAGAAGACGCTGCCCAA ACACCCAGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAGGAAAGAATCAAGGTACAGGGAAGT CCCTTGAAGAAACTCTTCAGTAGCTCAGGCTTAAAGAAGCTGTCTGGGAAGAAGCAGAAGGGGAAACGAG GAGGTGGGGGAGACGAAGAGCCTGGAGAATACCAACACATTCACACCGAATCCCCAGAGAGTGCTGATGA GCAGAAGGGAGAGAGCTCTGCGTCGTCCCCCGAGGAGCCTGAGGAGACCACGTGTCTGGAGAAAGGGCCG CTGGAAGCACCCCAGGATGGGGAAGCTGAGGAAGGAACTACTTCCGATGGAGAGAAGAAGAGGGAAGGGA TCACTCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAAGAAACGGGTCCGAAGACCTTCTGAGAGTGA CAAGGAGGAAGAGCTGGAGAAGGTCAAGAGCGCCACCTTGTCCTCCACTGATAGCACAGTGTCAGAAATG CAAGATGAAGTCAAAACTGTTGGTGAGGAACAAAAGCCAGAGGAACCAAAGCGTAGGGTGGATACTTCAG TGTCTTGGGAAGCACTGATTTGTGTCGGATCATCCAAGAAGAGAGCAAGGAAGGCATCCTCTTCAGATGA TGAAGGAGGGCCAAGGACACTGGGAGGGGACAGTCACAGAGCAGAGGAGGCCAGCAAAGACAAAGAAGCC GGAACAGACGCTGTTCCTGCCAGCACCCAGGAGCAGGACCAAGCGCAAGGAAGTTCCTCACCCGAGCCAG CGGGAAGCCCTTCCGAAGGGGAAGGTGTCTCCACTTGGGAGTCATTTAAAAGATTAGTCACTCCAAGAAA AAAATCCAAGTCAAAACTGGAAGAGAAAGCCGAAGACTCTAGTGTAGAGCAGTTGTCCACTGAGATCGAA CCGAGTAGAGAAGAATCTTGGGTTTCCATTAAGAAATTCATCCCCGGACGGCGGAAGAAAAGGGCAGATG GGAAGCAAGAACAAGCCACTGTGGAAGACTCAGGGCCAGTGGAGATAAATGAGGACGACCCTGATGTCCC AGCCGTCGTGCCTCTGTCTGAGTATGATGCAGTGGAGAGGGAGAAGATGGAAGCCCAGGGGAATGCGGAG CTGCCCCAGCTGCTGGGGGCTGTGTACGTGTCCGAGGAGCTCAGTAAGACTCTGGTCCACACTGTGAGTG TCGCAGTCATTGATGGGACCAGGGCAGTCACCAGTGTCGAAGAGCGGTCTCCTTCGTGGATATCCGCTTC CGTAACAGAACCTCTTGAACACACAGCGGGAGAAGCCATGCCACCTGTTGAAGAGGTCACTGAAAAAGAC ATCATTGCAGAAGAAACTCCTGTGCTCACCCAGACGTTACCAGAGGGTAAAGATGCCCATGACGACATGG TCACCAGTGAAGTGGATTTCACCTCAGAAGCTGTGACAGCCACAGAGACCTCAGAGGCTCTCCGTACTGA AGAAGTTACCGAAGCATCGGGGGCCGAAGAGACCACAGACATGGTGTCCGCAGTTTCCCAGCTGACTGAC TCCCCAGACACCACAGAGGAAGCCACCCCAGTTCAGGAGGTAGAGGGTGGTGTGCTAGATACAGAAGAAG AGGAGCGCCAGACGCAGGCCATCCTCCAAGCCGTTGCAGACAAGGTGAAAGAGGAGTCCCAGGTGCCTGC AACCCAGACTGTGCAGAGAACGGGGTCAAAAGCACTGGAGAAGGTTGAGGAGGTAGAGGAGGACTCCGAA GTGCTGGCTTCGGAGAAAGAGAAGGACGTTATGCCGAAAGGACCCGTGCAGGAAGCTGGAGCTGAGCATC TTGCACAGGGCTCTGAGACTGGACAGGCTACTCCAGAGAGCCTTGAAGTTCCTGAAGTCACAGCAGATGT AGACCATGTCGCCACGTGCCAGGTTATCAAGCTCCAGCAGCTGATGGAACAGGCCGTGGCCCCTGAGTCA TCCGAAACCTTGACAGACAGTGAGACAAATGGAAGCACTCCCTTAGCAGATTCAGACACTGCAGATGGGA CACAGCAAGATGAAACCATTGACAGCCAGGACAGTAAAGCCACTGCAGCTGTCAGGCAGTCACAGGTCAC AGAAGAAGAGGCGGCTACTGCTCAGAAAGAGGAGCCTTCGACACTACCTAATAATGTTCCAGCCCAGGAA GAACATGGGGAAGAACCAGGAAGAGATGTTCTTGAACCTACACAGCAAGAGCTTGCTGCTGCAGCCGTGC CCGTTCTGGCAAAGACTGAGGTGGGTCAAGAGGGTGAGGTTGACTGGTTGGATGGAGAAAAAGTCAAAGA AGAACAGGAGGTGTTTGTACACTCTGGACCCAACAGTCAAAAGGCTGCTGATGTGACATATGACAGTGAA GTGATGGGAGTGGCCGGGTGTCAGGAAAAGGAGAGTACTGAAGTGCAGAGTCTTAGCCTGGAGGAGGGAG AGATGGAAACTGACGTTGAAAAGGAGAAAAGGGAGACAAAGCCAGAGCAAGTGAGTGAAGAAGGTGAGCA GGAAACAGCCGCTCCTGAGCATGAAGGAACCTACGGGAAGCCAGTCCTGACACTTGACATGCCCAGCTCA GAGAGGGGGAAGGCACTGGGAAGCCTTGGAGGAAGCCCTTCTCTCCCAGACCAAGACAAAGCAGGTTGCA TAGAGGTTCAAGTTCAAAGCCTGGACACAACAGTCACTCAAACAGCAGAAGCTGTGGAAAAGGTCATAGA AACGGTTGTGATTTCAGAGACAGGTGAAAGTCCAGAGTGTGTAGGTGCACACTTATTACCAGCTGAGAAG TCCTCTGCAACGGGTGGCCACTGGACTCTTCAGCATGCAGAGGACACGGTACCCCTGGGGCCTGAGTCTC AGGCAGAATCCATCCCAATCATAGTAACTCCTGCTCCTGAAAGCACCCTACATCCTGACCTACAAGGAGA AATAAGCGCATCCCAGAGAGAGCGATCAGAGGAAGAGGACAAGCCAGATGCTGGTCCTGATGCTGACGGC AAGGAGAGTACAGCAATCGAAAAAGTCCTCAAGGCTGAACCTGAGATCCTGGAACTTGAGAGTAAGAGCA ACAAGATTGTGCTGAACGTCATTCAGACAGCCGTTGACCAGTTCGCACGTACAGAAACAGCCCCCGAAAC TCATGCTTATGATTCACAGACCCAGGTTCCTGCAATGCAGGCTGACAGCCAGGGAGCCCAACAGATGCTG GACAAAAATGAAAGTTGCCAAGATGAAACACCCAGTGCCGCAGCCCAGAGAGGACTTGCAAGTCCTGACC GTTCTGGAGGCATGGGCTCAGCCTCGGAAATGCTTGCCGCGCTTGCAGTTGAAAGCGCCGGTGTCAAAGT AAGCATTGAGAAGCTGCCTCCTCAACCCAAAGATCAAAAGGAGCATGCTGCTGATGGCCCTCAGCTCCAA AGCTTAGCCCAGGCAGAGGCCAGTGCCTCTGGAAACCTAACCAAAGAATCCCCAGACACCAACGGACCAA AGCTAACCGAGGAGGGCGATGCCCCAAAAGTTGAGGTCCAGGAAGAAGAAATGTCTACCAAGTCAGTCAA AGAGAACAAGGCCCAGGCAGAAGAGGACCTGCAGGAGCCAAAGGGAGACCTGGCAGAATCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001033653
Insert Size	4824 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001033653.1, NP_001028825.1
RefSeq Size	6026 bp
RefSeq ORF	4824 bp
Locus ID	83425
UniProt ID	Q5QD51
Gene Summary	Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.