Akap12 (NM_001033653) Rat Tagged ORF Clone
CAT#: RR200267
- TrueORF®
Akap12 (Myc-DDK-tagged ORF) - Rat A kinase (PRKA) anchor protein 12 (Akap12), transcript variant 2, (10 ug)
"NM_001033653" in other vectors (1)
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CNY 25,750.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AKAP12A; AKAP12B; AKAP12G |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200267 representing NM_001033653
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGGACCATTACCATCACAGTTGGACAGCGAGAGTCAGAAGATGTGAGAGAAAAAGACCGAGTTG AAGAAATGGCGGCCAACTCCACAGCTGTTGAAGATATCACAAAGGATGGGCAGGAGGAGACATCAGAAAT AATTGAACAGATCCCTGCTTCAGAAAGCAATGTGGAAGAAATGGTACAGCCTGCTGAGTCCCAGGCTAAT GATGTTGGCTTCAAGAAAGTATTTAAATTTGTTGGTTTTAAATTCACGGTGAAGAAGGATAAAAATGAAA AGTCAGATACTGTCCAACTACTCACTGTCAAGAAGGATGAAGGCGAAGGGGCAGAAGCCTCTGTCGGAGC TGGAGACCACCAGGAGCCCAGTGTGGAGACTGCCGTCGGAGAGTCAGCATCCAAAGAAAGTGAGCTGAAG CAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGAACAGAGCAGCACAGAAATCCCCCTTCAAGCCG AATCTGATCAAGCGGCTGAGGAAGAAGCCAAAGATGAAGGAGAAGAAAAACAAGAGAAAGAGCCCACCAA GTCCCCAGAATCCCCGAGCAGCCCAGTCAGCAGTGAGACAACATCTTCCTTCAAGAAGTTCTTCACTCAC GGTTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAATCAAAAGAGGATGATCTGGAAACTGCCGAGA AGAGAAAGGAGCAAGAGGCAGAAAAAGTAGACGAGGAAGAAAAGGAAAAGACAGAGCCAGCCTCGGAGGA GCAGGAGCCGGCAGAAGACACAGACCAGGCCAGGTTGTCAGCAGACTACGAGAAGGTGGAGCTGCCTTTG GAAGACCAGGTTGGTGACCTGGAGGCATCGTCAGAGGAGAAGTGTGCTCCTTTGGCAACGGAAGTGTTTG ATGAGAAGATGGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCACGTGAGCACCGTGGAGAAGACAGAGGA GGAGCAGGGAGGAGGAGGAGAGGCTGAAGGGGGCGTGGTGGTAGAAGGAACAGGAGAATCCTTGCCCCCT GAGAAACTGGCTGAGCCCCAGGAGGTCCCCCAGGAAGCTGAGCCTGCTGAGGAGCTGATGAAGAGCAGAG AGATGTGTGTCTCTGGAGGAGACCACACTCAACTGACAGACCTAAGTCCTGAAGAGAAGACGCTGCCCAA ACACCCAGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAGGAAAGAATCAAGGTACAGGGAAGT CCCTTGAAGAAACTCTTCAGTAGCTCAGGCTTAAAGAAGCTGTCTGGGAAGAAGCAGAAGGGGAAACGAG GAGGTGGGGGAGACGAAGAGCCTGGAGAATACCAACACATTCACACCGAATCCCCAGAGAGTGCTGATGA GCAGAAGGGAGAGAGCTCTGCGTCGTCCCCCGAGGAGCCTGAGGAGACCACGTGTCTGGAGAAAGGGCCG CTGGAAGCACCCCAGGATGGGGAAGCTGAGGAAGGAACTACTTCCGATGGAGAGAAGAAGAGGGAAGGGA TCACTCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAAGAAACGGGTCCGAAGACCTTCTGAGAGTGA CAAGGAGGAAGAGCTGGAGAAGGTCAAGAGCGCCACCTTGTCCTCCACTGATAGCACAGTGTCAGAAATG CAAGATGAAGTCAAAACTGTTGGTGAGGAACAAAAGCCAGAGGAACCAAAGCGTAGGGTGGATACTTCAG TGTCTTGGGAAGCACTGATTTGTGTCGGATCATCCAAGAAGAGAGCAAGGAAGGCATCCTCTTCAGATGA TGAAGGAGGGCCAAGGACACTGGGAGGGGACAGTCACAGAGCAGAGGAGGCCAGCAAAGACAAAGAAGCC GGAACAGACGCTGTTCCTGCCAGCACCCAGGAGCAGGACCAAGCGCAAGGAAGTTCCTCACCCGAGCCAG CGGGAAGCCCTTCCGAAGGGGAAGGTGTCTCCACTTGGGAGTCATTTAAAAGATTAGTCACTCCAAGAAA AAAATCCAAGTCAAAACTGGAAGAGAAAGCCGAAGACTCTAGTGTAGAGCAGTTGTCCACTGAGATCGAA CCGAGTAGAGAAGAATCTTGGGTTTCCATTAAGAAATTCATCCCCGGACGGCGGAAGAAAAGGGCAGATG GGAAGCAAGAACAAGCCACTGTGGAAGACTCAGGGCCAGTGGAGATAAATGAGGACGACCCTGATGTCCC AGCCGTCGTGCCTCTGTCTGAGTATGATGCAGTGGAGAGGGAGAAGATGGAAGCCCAGGGGAATGCGGAG CTGCCCCAGCTGCTGGGGGCTGTGTACGTGTCCGAGGAGCTCAGTAAGACTCTGGTCCACACTGTGAGTG TCGCAGTCATTGATGGGACCAGGGCAGTCACCAGTGTCGAAGAGCGGTCTCCTTCGTGGATATCCGCTTC CGTAACAGAACCTCTTGAACACACAGCGGGAGAAGCCATGCCACCTGTTGAAGAGGTCACTGAAAAAGAC ATCATTGCAGAAGAAACTCCTGTGCTCACCCAGACGTTACCAGAGGGTAAAGATGCCCATGACGACATGG TCACCAGTGAAGTGGATTTCACCTCAGAAGCTGTGACAGCCACAGAGACCTCAGAGGCTCTCCGTACTGA AGAAGTTACCGAAGCATCGGGGGCCGAAGAGACCACAGACATGGTGTCCGCAGTTTCCCAGCTGACTGAC TCCCCAGACACCACAGAGGAAGCCACCCCAGTTCAGGAGGTAGAGGGTGGTGTGCTAGATACAGAAGAAG AGGAGCGCCAGACGCAGGCCATCCTCCAAGCCGTTGCAGACAAGGTGAAAGAGGAGTCCCAGGTGCCTGC AACCCAGACTGTGCAGAGAACGGGGTCAAAAGCACTGGAGAAGGTTGAGGAGGTAGAGGAGGACTCCGAA GTGCTGGCTTCGGAGAAAGAGAAGGACGTTATGCCGAAAGGACCCGTGCAGGAAGCTGGAGCTGAGCATC TTGCACAGGGCTCTGAGACTGGACAGGCTACTCCAGAGAGCCTTGAAGTTCCTGAAGTCACAGCAGATGT AGACCATGTCGCCACGTGCCAGGTTATCAAGCTCCAGCAGCTGATGGAACAGGCCGTGGCCCCTGAGTCA TCCGAAACCTTGACAGACAGTGAGACAAATGGAAGCACTCCCTTAGCAGATTCAGACACTGCAGATGGGA CACAGCAAGATGAAACCATTGACAGCCAGGACAGTAAAGCCACTGCAGCTGTCAGGCAGTCACAGGTCAC AGAAGAAGAGGCGGCTACTGCTCAGAAAGAGGAGCCTTCGACACTACCTAATAATGTTCCAGCCCAGGAA GAACATGGGGAAGAACCAGGAAGAGATGTTCTTGAACCTACACAGCAAGAGCTTGCTGCTGCAGCCGTGC CCGTTCTGGCAAAGACTGAGGTGGGTCAAGAGGGTGAGGTTGACTGGTTGGATGGAGAAAAAGTCAAAGA AGAACAGGAGGTGTTTGTACACTCTGGACCCAACAGTCAAAAGGCTGCTGATGTGACATATGACAGTGAA GTGATGGGAGTGGCCGGGTGTCAGGAAAAGGAGAGTACTGAAGTGCAGAGTCTTAGCCTGGAGGAGGGAG AGATGGAAACTGACGTTGAAAAGGAGAAAAGGGAGACAAAGCCAGAGCAAGTGAGTGAAGAAGGTGAGCA GGAAACAGCCGCTCCTGAGCATGAAGGAACCTACGGGAAGCCAGTCCTGACACTTGACATGCCCAGCTCA GAGAGGGGGAAGGCACTGGGAAGCCTTGGAGGAAGCCCTTCTCTCCCAGACCAAGACAAAGCAGGTTGCA TAGAGGTTCAAGTTCAAAGCCTGGACACAACAGTCACTCAAACAGCAGAAGCTGTGGAAAAGGTCATAGA AACGGTTGTGATTTCAGAGACAGGTGAAAGTCCAGAGTGTGTAGGTGCACACTTATTACCAGCTGAGAAG TCCTCTGCAACGGGTGGCCACTGGACTCTTCAGCATGCAGAGGACACGGTACCCCTGGGGCCTGAGTCTC AGGCAGAATCCATCCCAATCATAGTAACTCCTGCTCCTGAAAGCACCCTACATCCTGACCTACAAGGAGA AATAAGCGCATCCCAGAGAGAGCGATCAGAGGAAGAGGACAAGCCAGATGCTGGTCCTGATGCTGACGGC AAGGAGAGTACAGCAATCGAAAAAGTCCTCAAGGCTGAACCTGAGATCCTGGAACTTGAGAGTAAGAGCA ACAAGATTGTGCTGAACGTCATTCAGACAGCCGTTGACCAGTTCGCACGTACAGAAACAGCCCCCGAAAC TCATGCTTATGATTCACAGACCCAGGTTCCTGCAATGCAGGCTGACAGCCAGGGAGCCCAACAGATGCTG GACAAAAATGAAAGTTGCCAAGATGAAACACCCAGTGCCGCAGCCCAGAGAGGACTTGCAAGTCCTGACC GTTCTGGAGGCATGGGCTCAGCCTCGGAAATGCTTGCCGCGCTTGCAGTTGAAAGCGCCGGTGTCAAAGT AAGCATTGAGAAGCTGCCTCCTCAACCCAAAGATCAAAAGGAGCATGCTGCTGATGGCCCTCAGCTCCAA AGCTTAGCCCAGGCAGAGGCCAGTGCCTCTGGAAACCTAACCAAAGAATCCCCAGACACCAACGGACCAA AGCTAACCGAGGAGGGCGATGCCCCAAAAGTTGAGGTCCAGGAAGAAGAAATGTCTACCAAGTCAGTCAA AGAGAACAAGGCCCAGGCAGAAGAGGACCTGCAGGAGCCAAAGGGAGACCTGGCAGAATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200267 representing NM_001033653
Red=Cloning site Green=Tags(s) MLGTITITVGQRESEDVREKDRVEEMAANSTAVEDITKDGQEETSEIIEQIPASESNVEEMVQPAESQAN DVGFKKVFKFVGFKFTVKKDKNEKSDTVQLLTVKKDEGEGAEASVGAGDHQEPSVETAVGESASKESELK QSTEKQEGTLKQEQSSTEIPLQAESDQAAEEEAKDEGEEKQEKEPTKSPESPSSPVSSETTSSFKKFFTH GWAGWRKKTSFKKSKEDDLETAEKRKEQEAEKVDEEEKEKTEPASEEQEPAEDTDQARLSADYEKVELPL EDQVGDLEASSEEKCAPLATEVFDEKMEAHQEVVAEVHVSTVEKTEEEQGGGGEAEGGVVVEGTGESLPP EKLAEPQEVPQEAEPAEELMKSREMCVSGGDHTQLTDLSPEEKTLPKHPEGIVSEVEMLSSQERIKVQGS PLKKLFSSSGLKKLSGKKQKGKRGGGGDEEPGEYQHIHTESPESADEQKGESSASSPEEPEETTCLEKGP LEAPQDGEAEEGTTSDGEKKREGITPWASFKKMVTPKKRVRRPSESDKEEELEKVKSATLSSTDSTVSEM QDEVKTVGEEQKPEEPKRRVDTSVSWEALICVGSSKKRARKASSSDDEGGPRTLGGDSHRAEEASKDKEA GTDAVPASTQEQDQAQGSSSPEPAGSPSEGEGVSTWESFKRLVTPRKKSKSKLEEKAEDSSVEQLSTEIE PSREESWVSIKKFIPGRRKKRADGKQEQATVEDSGPVEINEDDPDVPAVVPLSEYDAVEREKMEAQGNAE LPQLLGAVYVSEELSKTLVHTVSVAVIDGTRAVTSVEERSPSWISASVTEPLEHTAGEAMPPVEEVTEKD IIAEETPVLTQTLPEGKDAHDDMVTSEVDFTSEAVTATETSEALRTEEVTEASGAEETTDMVSAVSQLTD SPDTTEEATPVQEVEGGVLDTEEEERQTQAILQAVADKVKEESQVPATQTVQRTGSKALEKVEEVEEDSE VLASEKEKDVMPKGPVQEAGAEHLAQGSETGQATPESLEVPEVTADVDHVATCQVIKLQQLMEQAVAPES SETLTDSETNGSTPLADSDTADGTQQDETIDSQDSKATAAVRQSQVTEEEAATAQKEEPSTLPNNVPAQE EHGEEPGRDVLEPTQQELAAAAVPVLAKTEVGQEGEVDWLDGEKVKEEQEVFVHSGPNSQKAADVTYDSE VMGVAGCQEKESTEVQSLSLEEGEMETDVEKEKRETKPEQVSEEGEQETAAPEHEGTYGKPVLTLDMPSS ERGKALGSLGGSPSLPDQDKAGCIEVQVQSLDTTVTQTAEAVEKVIETVVISETGESPECVGAHLLPAEK SSATGGHWTLQHAEDTVPLGPESQAESIPIIVTPAPESTLHPDLQGEISASQRERSEEEDKPDAGPDADG KESTAIEKVLKAEPEILELESKSNKIVLNVIQTAVDQFARTETAPETHAYDSQTQVPAMQADSQGAQQML DKNESCQDETPSAAAQRGLASPDRSGGMGSASEMLAALAVESAGVKVSIEKLPPQPKDQKEHAADGPQLQ SLAQAEASASGNLTKESPDTNGPKLTEEGDAPKVEVQEEEMSTKSVKENKAQAEEDLQEPKGDLAES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001033653 |
ORF Size | 4821 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001033653.1, NP_001028825.1 |
RefSeq Size | 6026 bp |
RefSeq ORF | 4824 bp |
Locus ID | 83425 |
UniProt ID | Q5QD51 |
MW | 173.1 kDa |
Gene Summary | Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200267 | Akap12 (untagged ORF) - Rat A kinase (PRKA) anchor protein 12 (Akap12), transcript variant 2, (10 ug) |
CNY 25,750.00 |