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LIMCH1 (NM_001289122) Human Tagged ORF Clone
CAT＃: RG239935
- TrueORF®
LIMCH1 (tGFP-tagged) - Human LIM and calponin homology domains 1 (LIMCH1), transcript variant 6
ORF Plasmid: DDK
"NM_001289122" in other vectors (2)
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CNY 11,020.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LIMCH1A; LMO7B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239935 representing NM_001289122.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTTGTCCCGCTCTCGGTCTGGAAGCTCTTCAGCCCCTGCAGCCCGAGCCGCCCCCCGAGCCCGCC TTCTCCGAGGCGCAGAAGTGGATTGAGCAAGTAACTGGCAGAAGTTTTGGTGATAAAGATTTTCGGACA GGTTTAGAAAATGGAATCCTCCTCTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATC AATAGATTGCCTACCCCCATTGCAGGACTGGACAATATTATCTTATTCTTGAGAGGTTGTAAAGAGCTC GGCCTTAAAGAATCTCAACTTTTTGACCCGAGTGACCTCCAGGATACATCCAACAGAGTAACAGTCAAG AGCCTTGATTATAGTAGGAAGCTGAAAAATGTATTAGTTACCATTTACTGGCTGGGAAAAGCAGCAAAC AGCTGCACATCCTACAGCGGAACGACACTAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGA AAGGACACTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCTGGGAT TCCGAGCGCAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGATTCC TTTGGCTCTCGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGAGGA AGCGACTCTGAATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGG CGGACTTCCCATGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCAG ACGGCCTACGTCCCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGT ACCGCCACCTCCCCGCTGGGTGGGGAGAGGCCCTTCAGGAGCACCAGCATGTTTGACATGCGGTGTGAG GAGGAGGCCGCGGTGCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTG AGGGAAGAGGACGACAAATGGCAAGATGACCTGGCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAG GACTTAATCAAGAAAGAGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGCTGGAGAAGATGGGACAAGT GAACGAAGGAAAAGCATCAAAACCTACAGAGAAATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTG CATGAAGCATATAAGAACGCTCGGTCCCAGGAGGAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGG TTCACCATCAGTGAGGCTGTTCTCGAACGCTTGGAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACA GAGCCAAATTTATCCTCCTTCCTGAATGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTGCCT CCACCCAAATTCACTGCCACTGTTGAAACCACCATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCA GCAGGCAGTGGGTCTCCAAGCAAAACTGTCACTCCCAAAGCAGTGCCTATGCTGACACCCAAGCCTTAC TCCCAGCCCAAAAATTCTCAAGATGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTCAGTGTGAATGGA GAGACGGTTCATAGAGAGGAGGAGAAGGAAAGAGAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACC AAATCCCAGATGTTTGAAGGTGTGGCCAGAGTGCACGGGTCTCCACTGGAGCTGAAACAAGACAACGGT AGCATCGAGATCAACATAAAGAAGCCAAACTCTGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACG GAACCGAATAGTCAAGAGGACAAGAATGATGGTGGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCA TCAGAACCACAGCATTTTACAACAACTGTGACTCGATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCC TCCAGCCCCCAGCTGAAGAATGATGTGTCGGAAGAAAAAGACCAGAAGAAACCAGAAAATGAAATGAGT GGAAAGGTGGAGTTGGTGCTGTCACAAAAGGTGGTAAAGCCAAAATCTCCAGAACCCGAAGCAACGCTG ACATTTCCATTTCTGGACAAAATGCCTGAAGCCAACCAACTACATTTGCCAAATCTCAATTCTCAAGAG TCGCCTGGCACTGCCAGTGTTCCGCTTAGAGTTCAGAACTCTTGGCGACGATCCCAGTTTTTCTCCCAG TCAGTGGATTCTCCAAGCAGTGAGAAGTCACCTGTTATGACACCTTTTAAGTTCTGGGCATGGGACCCA GAAGAGGAGCGCAGGCGACAGGAAAAATGGCAACAGGAACAGGAACGTTTGCTCCAGGAGAGATACCAG AAGGAGCAGGACAAGCTGAAAGAAGAGTGGGAAAAGGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGA TACTATGAGGAGGAGCGTAAGATAATTGAAGACACTGTGGTTCCATTTACTGTTTCTTCAAGTTCCGCT GACCAGCTGTCTACCTCTTCCTCCATGACTGAAGGCAGTGGGACAATGAATAAGATAGACCTGGGAAAC TGTCAAGATGAAAAACAAGACAGAAGATGGAAGAAATCATTCCAGGGAGATGACAGTGACTTATTGCTG AAGACTAGGGAAAGTGATCGACTGGAGGAGAAGGGCAGCCTAACTGAAGGGGCCTTGGCTCATTCTGGG AACCCTGTATCAAAAGGAGTCCATGAAGACCATCAGCTGGATACCGAGGCTGGGGCCCCACACTGTGGA ACAAACCCACAGCTTGCTCAGGATCCATCCCAGAATCAGCAGACATCAAATCCAACGCACAGTTCAGAA GATGTGAAGCCAAAAACCCTCCCGCTGGATAAAAGCATTAACCATCAGATCGAGTCTCCCAGTGAAAGG CGGAAGTCTATAAGTGGAAAGAAGCTGTGCTCTTCCTGTGGGCTTCCTTTGGGTAAAGGAGCTGCAATG ATCATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAGGTGTGGAATTTGTAAAGGCCAGCTT GGAGATGCAGTGAGTGGGACGGATGTTAGGATTCGAAATGGTCTCCTGAACTGTAATGATTGCTACATG CGATCCAGAAGTGCCGGGCAGCCTACAACATTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239935
Blue=ORF Red=Cloning site Green=Tag(s) MACPALGLEALQPLQPEPPPEPAFSEAQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKI NRLPTPIAGLDNIILFLRGCKELGLKESQLFDPSDLQDTSNRVTVKSLDYSRKLKNVLVTIYWLGKAAN SCTSYSGTTLNLKEFEGLLAQMRKDTDDIESPKRSIRDSGYIDCWDSERSDSLSPPRHGRDDSFDSLDS FGSRSRQTPSPDVVLRGSSDGRGSDSESDLPHRKLPDVKKDDMSARRTSHGEPKSAVPFNQYLPNKSNQ TAYVPAPLRKKKAEREEYRKSWSTATSPLGGERPFRSTSMFDMRCEEEAAVQPHSRARQEQLQLINNQL REEDDKWQDDLARWKSRRRSVSQDLIKKEEERKKMEKLLAGEDGTSERRKSIKTYREIVQEKERREREL HEAYKNARSQEEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNLSSFLNDPNPMKYLRQQSLP PPKFTATVETTIARASVLDTSMSAGSGSPSKTVTPKAVPMLTPKPYSQPKNSQDVLKTFKVDGKVSVNG ETVHREEEKERECPTVAPAHSLTKSQMFEGVARVHGSPLELKQDNGSIEINIKKPNSVPQELAATTEKT EPNSQEDKNDGGKSRKGNIELASSEPQHFTTTVTRCSPTVAFVEFPSSPQLKNDVSEEKDQKKPENEMS GKVELVLSQKVVKPKSPEPEATLTFPFLDKMPEANQLHLPNLNSQESPGTASVPLRVQNSWRRSQFFSQ SVDSPSSEKSPVMTPFKFWAWDPEEERRRQEKWQQEQERLLQERYQKEQDKLKEEWEKAQKEVEEEERR YYEEERKIIEDTVVPFTVSSSSADQLSTSSSMTEGSGTMNKIDLGNCQDEKQDRRWKKSFQGDDSDLLL KTRESDRLEEKGSLTEGALAHSGNPVSKGVHEDHQLDTEAGAPHCGTNPQLAQDPSQNQQTSNPTHSSE DVKPKTLPLDKSINHQIESPSERRKSISGKKLCSSCGLPLGKGAAMIIETLNLYFHIQCFRCGICKGQL GDAVSGTDVRIRNGLLNCNDCYMRSRSAGQPTTL TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001289122 |
| ORF Size | 3207 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001289122.3 |
| RefSeq Size | 6142 bp |
| RefSeq ORF | 3210 bp |
| Locus ID | 22998 |
| UniProt ID | Q9UPQ0 |
| MW | 120.9 kDa |
| Gene Summary | Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function] |
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