LIMCH1 (NM_001289122) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LIMCH1A; LMO7B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289122, the custom clone sequence may differ by one or more nucleotides ATGGCTTGTCCCGCTCTCGGTCTGGAAGCTCTTCAGCCCCTGCAGCCCGAGCCGCCCCCCGAGCCCGCCT TCTCCGAGGCGCAGAAGTGGATTGAGCAAGTAACTGGCAGAAGTTTTGGTGATAAAGATTTTCGGACAGG TTTAGAAAATGGAATCCTCCTCTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAAT AGATTGCCTACCCCCATTGCAGGACTGGACAATATTATCTTATTCTTGAGAGGTTGTAAAGAGCTCGGCC TTAAAGAATCTCAACTTTTTGACCCGAGTGACCTCCAGGATACATCCAACAGAGTAACAGTCAAGAGCCT TGATTATAGTAGGAAGCTGAAAAATGTATTAGTTACCATTTACTGGCTGGGAAAAGCAGCAAACAGCTGC ACATCCTACAGCGGAACGACACTAAACCTGAAGGAGTTTGAAGGATTGTTGGCTCAGATGCGAAAGGACA CTGATGACATTGAAAGTCCTAAACGCAGTATCCGAGACAGTGGCTACATCGACTGCTGGGATTCCGAGCG CAGCGACTCCCTCTCTCCTCCTCGCCACGGCAGAGATGATTCCTTCGACAGCCTGGATTCCTTTGGCTCT CGCTCTCGGCAGACGCCTTCACCAGATGTAGTCCTCAGGGGAAGCAGCGATGGGAGAGGAAGCGACTCTG AATCCGACTTGCCTCATCGGAAGCTGCCAGATGTGAAGAAGGATGACATGTCTGCACGGCGGACTTCCCA TGGTGAGCCGAAATCAGCAGTGCCTTTTAACCAGTACCTCCCGAACAAAAGCAATCAGACGGCCTACGTC CCCGCGCCTCTGAGAAAGAAGAAAGCAGAGAGAGAGGAATACCGCAAGAGCTGGAGTACCGCCACCTCCC CGCTGGGTGGGGAGAGGCCCTTCAGGAGCACCAGCATGTTTGACATGCGGTGTGAGGAGGAGGCCGCGGT GCAGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCTGATAAATAACCAGCTGAGGGAAGAGGACGAC AAATGGCAAGATGACCTGGCTCGTTGGAAGAGTCGTAGAAGAAGTGTTTCTCAGGACTTAATCAAGAAAG AGGAAGAAAGGAAAAAAATGGAGAAGTTACTGGCTGGAGAAGATGGGACAAGTGAACGAAGGAAAAGCAT CAAAACCTACAGAGAAATTGTTCAAGAAAAAGAGCGGAGAGAGAGAGAGCTGCATGAAGCATATAAGAAC GCTCGGTCCCAGGAGGAGGCAGAGGGGATCCTTCAACAGTACATTGAGAGGTTCACCATCAGTGAGGCTG TTCTCGAACGCTTGGAGATGCCAAAAATTCTGGAAAGAAGCCATTCAACAGAGCCAAATTTATCCTCCTT CCTGAATGACCCCAATCCCATGAAATACCTGCGGCAACAGTCACTGCCTCCACCCAAATTCACTGCCACT GTTGAAACCACCATTGCTCGTGCCAGTGTTCTGGATACCAGCATGTCAGCAGGCAGTGGGTCTCCAAGCA AAACTGTCACTCCCAAAGCAGTGCCTATGCTGACACCCAAGCCTTACTCCCAGCCCAAAAATTCTCAAGA TGTTCTGAAGACCTTTAAGGTAGACGGGAAAGTCAGTGTGAATGGAGAGACGGTTCATAGAGAGGAGGAG AAGGAAAGAGAGTGTCCCACGGTGGCACCTGCCCACTCCTTAACCAAATCCCAGATGTTTGAAGGTGTGG CCAGAGTGCACGGGTCTCCACTGGAGCTGAAACAAGACAACGGTAGCATCGAGATCAACATAAAGAAGCC AAACTCTGTTCCCCAAGAGCTCGCAGCAACCACTGAGAAAACGGAACCGAATAGTCAAGAGGACAAGAAT GATGGTGGAAAATCAAGAAAAGGGAATATAGAACTTGCCTCATCAGAACCACAGCATTTTACAACAACTG TGACTCGATGCAGCCCGACCGTGGCCTTTGTGGAATTTCCCTCCAGCCCCCAGCTGAAGAATGATGTGTC GGAAGAAAAAGACCAGAAGAAACCAGAAAATGAAATGAGTGGAAAGGTGGAGTTGGTGCTGTCACAAAAG GTGGTAAAGCCAAAATCTCCAGAACCCGAAGCAACGCTGACATTTCCATTTCTGGACAAAATGCCTGAAG CCAACCAACTACATTTGCCAAATCTCAATTCTCAAGAGTCGCCTGGCACTGCCAGTGTTCCGCTTAGAGT TCAGAACTCTTGGCGACGATCCCAGTTTTTCTCCCAGTCAGTGGATTCTCCAAGCAGTGAGAAGTCACCT GTTATGACACCTTTTAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCAGGCGACAGGAAAAATGGCAAC AGGAACAGGAACGTTTGCTCCAGGAGAGATACCAGAAGGAGCAGGACAAGCTGAAAGAAGAGTGGGAAAA GGCCCAAAAGGAGGTGGAAGAGGAAGAACGCAGATACTATGAGGAGGAGCGTAAGATAATTGAAGACACT GTGGTTCCATTTACTGTTTCTTCAAGTTCCGCTGACCAGCTGTCTACCTCTTCCTCCATGACTGAAGGCA GTGGGACAATGAATAAGATAGACCTGGGAAACTGTCAAGATGAAAAACAAGACAGAAGATGGAAGAAATC ATTCCAGGGAGATGACAGTGACTTATTGCTGAAGACTAGGGAAAGTGATCGACTGGAGGAGAAGGGCAGC CTAACTGAAGGGGCCTTGGCTCATTCTGGGAACCCTGTATCAAAAGGAGTCCATGAAGACCATCAGCTGG ATACCGAGGCTGGGGCCCCACACTGTGGAACAAACCCACAGCTTGCTCAGGATCCATCCCAGAATCAGCA GACATCAAATCCAACGCACAGTTCAGAAGATGTGAAGCCAAAAACCCTCCCGCTGGATAAAAGCATTAAC CATCAGATCGAGTCTCCCAGTGAAAGGCGGAAGTCTATAAGTGGAAAGAAGCTGTGCTCTTCCTGTGGGC TTCCTTTGGGTAAAGGAGCTGCAATGATCATCGAGACCCTCAATCTCTATTTTCACATCCAGTGTTTCAG GTGTGGAATTTGTAAAGGCCAGCTTGGAGATGCAGTGAGTGGGACGGATGTTAGGATTCGAAATGGTCTC CTGAACTGTAATGATTGCTACATGCGATCCAGAAGTGCCGGGCAGCCTACAACATTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289122
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289122.1, NP_001276051.1
RefSeq Size	6142 bp
RefSeq ORF	3210 bp
Locus ID	22998
UniProt ID	Q9UPQ0
Gene Summary	Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]

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