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MCM8 (NM_001281522) Human Tagged ORF Clone
CAT＃: RG239575
- TrueORF®
MCM8 (tGFP-tagged) - Human minichromosome maintenance complex component 8 (MCM8), transcript variant 5
ORF Plasmid: DDK
"NM_001281522" in other vectors (2)
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CNY 6,180.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C20orf154; dJ967N21.5; POF10 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239575 representing NM_001281522.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATGGAGAGTATAGAGGCAGAGGATTTGGACGAGGAAGATTTCAAAGCTGGAAAAGGGGAAGAGGT GGTGGGAACTTCTCAGGAAAATGGAGAGAAAGAGAACACAGACCTGATCTGAGTAAAACCACAGGAAAA CGTACTTCTGAACAAACCCCACAGTTTTTGCTTTCAACAAAGACCCCACAGTCAATGCAGTCAACATTG GATCGATTCATACCATATAAAGGCTGGAAGCTTTATTTCTCTGAAGTTTACAGCGATAGCTCTCCTTTG ATTGAGAAGATTCAAGCATTTGAAAAATTTTTCACAAGGCATATTGATTTGTATGACAAGGATGAAATA GAAAGAAAGGGAAGTATTTTGGTAGATTTTAAAGAACTGACAGAAGGTGGTGAAGTAACTAACTTGATA CCAGATATAGCAACTGAACTAAGAGATGCACCTGAGAAAACCTTGGCTTGCATGGGTTTGGCAATACAT CAGGTGTTAACTAAGGACCTTGAAAGGCATGCAGCTGAGTTACAAGCCCAGGAAGGATTGTCTAATGAT GGAGAAACAATGGTAAATGTGCCACATATTCATGCAAGGGTGTACAACTATGAGCCTTTGACACAGCTC AAGAATGTCAGAGCAAATTACTATGGAAAATACATTGCTCTAAGAGGGACAGTGGTTCGTGTCAGTAAT ATAAAGCCTCTTTGCACCAAGATGGCTTTTCTTTGTGCTGCATGTGGAGAAATTCAGAGCTTTCCTCTT CCAGATGGAAAATACAGTCTTCCCACAAAGTGTCCTGTGCCTGTGTGTCGAGGCAGGTCATTTACTGCT CTCCGCAGCTCTCCTCTCACAGTTACGATGGACTGGCAGTCAATCAAAATCCAGGAATTGATGTCTGAT GATCAGAGAGAAGCAGGTCGGATTCCACGAACAATAGAATGTGAGCTTGTTCATGATCTTGTGGATAGC TGTGTCCCGGGAGACACAGTGACTATTACTGGAATTGTCAAAGTCTCAAATGCGGAAGAAGGTTCTCGA AATAAGAATGACAAGTGTATGTTCCTTTTGTATATTGAAGCAAATTCTATTAGTAATAGCAAAGGACAG AAAACAAAGAGTTCTGAGGATGGGTGTAAGCATGGAATGTTGATGGAGTTCTCACTTAAAGACCTTTAT GCCATCCAAGAGATTCAAGCTGAAGAAAACCTGTTTAAACTCATTGTCAACTCGCTTTGCCCTGTCATT TTTGGTCATGAAGCAGCGTGCAATGTTGCCCCACGTGGCGTGTATGTTTGTGGTAACACCACGACCACC TCTGGTCTGACGGTAACTCTTTCAAAAGATAGTTCCTCTGGAGATTTTGCTTTGGAAGCTGGTGCCCTG GTACTTGGTGATCAAGGTATTTGTGGAATCGATGAATTTGATAAGATGGGGAATCAACATCAAGCCTTG TTGGAAGCCATGGAGCAGCAAAGTATTAGTCTTGCTAAGGCTGGTGTGGTTTGTAGCCTTCCTGCAAGA ACTTCCATTATTGCTGCTGCAAATCCAGTTGGAGGACATTACAATAAAGCCAAAACAGTTTCTGAGAAT TTAAAAATGGGGAGTGCACTACTATCCAGATTTGATTTGGTCTTTATCCTGTTAGATACTCCAAATGAG CATCATGATCACTTACTCTCTGAACATGTGATTGCAATAAGAGCTGGAAAGCAGAGAACCATTAGCAGT GCCACAGTAGCTCGTATGAATAGTCAAGATTCAAATACTTCCGTACTTGAAGTAGTTTCTGAGAAGCCA TTATCAGAAAGACTAAAGGTGGTTCCTGGAGAAACAATAGATCCCATTCCCCACCAGCTATTGAGAAAG TACATTGGCTATGCTCGGCAGTATGTGTACCCAAGGCTATCCACAGAAGCTGCTCGAGTTCTTCAAGAT TTTTACCTTGAGCTCCGGAAACAGAGCCAGAGGTTAAATAGCTCACCAATCACTACCAGGCAGCTGGAA TCTTTGATTCGTCTGACAGAGGCACGAGCAAGGTTGGAATTGAGAGAGGAAGCAACCAAAGAAGACGCT GAGGATATAGTGGAAATTATGAAATATAGCATGCTAGGAACTTACTCTGATGAATTTGGGAACCTAGAT TTTGAGCGATCCCAGCATGGTTCTGGAATGAGCAACAGGTCAACAGCGAAAAGATTTATTTCTGCTCTC AACAACGTTGCTGAAAGAACTTATAATAATATATTTCAATTTCATCAACTTCGGCAGATTGCCAAAGAA CTAAACATTCAGGTTGCTGATTTTGAAAATTTTATTGGATCACTAAATGACCAGGGTTACCTCTTGAAA AAAGGCCCAAAAGTTTACCAGCTTCAAACTATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239575
Blue=ORF Red=Cloning site Green=Tag(s) MNGEYRGRGFGRGRFQSWKRGRGGGNFSGKWREREHRPDLSKTTGKRTSEQTPQFLLSTKTPQSMQSTL DRFIPYKGWKLYFSEVYSDSSPLIEKIQAFEKFFTRHIDLYDKDEIERKGSILVDFKELTEGGEVTNLI PDIATELRDAPEKTLACMGLAIHQVLTKDLERHAAELQAQEGLSNDGETMVNVPHIHARVYNYEPLTQL KNVRANYYGKYIALRGTVVRVSNIKPLCTKMAFLCAACGEIQSFPLPDGKYSLPTKCPVPVCRGRSFTA LRSSPLTVTMDWQSIKIQELMSDDQREAGRIPRTIECELVHDLVDSCVPGDTVTITGIVKVSNAEEGSR NKNDKCMFLLYIEANSISNSKGQKTKSSEDGCKHGMLMEFSLKDLYAIQEIQAEENLFKLIVNSLCPVI FGHEAACNVAPRGVYVCGNTTTTSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQAL LEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNE HHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRK YIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDA EDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKE LNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001281522 |
| ORF Size | 2379 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001281522.1, NP_001268451.1 |
| RefSeq Size | 3574 bp |
| RefSeq ORF | 2382 bp |
| Locus ID | 84515 |
| UniProt ID | Q9UJA3 |
| Protein Families | Transcription Factors |
| MW | 89.2 kDa |
| Gene Summary | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013] |
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