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SAFB (NM_001201340) Human Tagged ORF Clone
CAT＃: RG234961
- TrueORF®
SAFB (tGFP-tagged) - Homo sapiens scaffold attachment factor B (SAFB), transcript variant 4
ORF Plasmid: DDK
"NM_001201340" in other vectors (2)
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CNY 7,890.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HAP; HET; SAB-B1; SAF-B; SAF-B1; SAFB1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234961 representing NM_001201340
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACTCTGTCAGGCCTAGGTGATTCTGGAGCGGCGGGCGCGGCGGCTCTGAGCTCCGCCTCGT CAGAGACCGGGACGCGGCGCCTCAGCGACCTGCGAGTGATCGATCTGCGGGCGGAGCTGAGGAAACGGAA TGTGGACTCGAGCGGCAACAAGAGCGTTTTGATGGAGCGGCTGAAGAAGGCAATTGAAGATGAAGGTGGT AATCCTGACGAAATTGAAATTACCTCCGAGGGAAACAAGAAAACATCAAAGAGGTCTAGCAAAGGGCGCA AACCAGAAGAAGAGGGTGTGGAAGATAACGGGCTGGAGGAAAACTCTGGGGATGGACAGATAGAGGACAA AGAAACTATAAACAATTTAGATACTTCATCATCTGACTTCACTATATTACAGGAAATTGAAGAGCCATCC CTGGAGCCAGAAAATGAGAAAATACTCGACATTTTGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAG AAAGTTCCGAGCTGGAGCAGCCATTTGCACAGGACACAAGTAGCGTGGGGCCAGACAGAAAGCTTGCGGA GGAAGAGGACCTATTTGACAGCGCCCATCCGGAAGAGGGTGATTTAGATTTGGCCAGCGAGTCAACAGCA CACGCTCAGTCGAGCAAGGCAGACAGCCTGTTAGCGGTAGTGAAAAGGGAGCCCGCGGAGCAGCCAGGCG ATGGCGAGAGGACGGACTGTGAGCCTGTAGGGCTAGAGCCGGCAGTTGAGCAGAGTAGTGCGGCCTCCGA GCTCGCGGAGGCCTCTAGCGAGGAGCTCGCAGAAGCACCCACGGAAGCCCCAAGCCCAGAAGCCAGAGAT AGCAAAGAAGACGGGAGGAAGTTTGATTTTGACGCTTGTAATGAAGTCCCTCCGGCTCCTAAAGAGTCCT CAACCAGTGAGGGCGCTGATCAGAAAATGAGTTCTCCCGAAGATGACTCGGATACAAAAAGGCTTTCCAA AGAGGAAAAGGGTCGCAGCAGTTGTGGTAGAAATTTCTGGGTTAGTGGACTCTCTTCTACAACCAGAGCT ACAGATTTGAAGAATCTTTTCAGCAAATATGGGAAGGTGGTGGGCGCCAAGGTTGTGACAAATGCCCGGA GTCCTGGAGCTCGCTGTTACGGTTTTGTCACGATGTCCACAGCAGAAGAGGCCACAAAATGCATTAACCA CCTGCACAAGACGGAGCTCCACGGAAAGATGATCTCCGTGGAGAAAGCCAAAAATGAACCTGTGGGAAAG AAAACCTCTGACAAAAGAGACAGTGACGGGAAAAAGGAGAAGTCGAGCAACAGTGACAGATCTACAAACC TTAAGAGGGATGATAAATGTGACAGAAAAGATGATGCTAAGAAGGGTGACGACGGAAGTGGAGAAAAGAG TAAGGACCAAGATGATCAGAAACCTGGCCCCTCAGAGCGATCTCGAGCCACAAAGTCAGGAAGTCGAGGG ACCGAACGGACTGTAGTAATGGATAAATCCAAAGGGGTGCCTGTGATTAGTGTAAAAACGTCCGGGTCCA AAGAGAGAGCTTCCAAAAGCCAGGATCGCAAATCAGCCAGCAGAGAGAAGCGGTCCGTCGTGTCCTTTGA TAAGGTCAAGGAGCCTCGGAAGTCAAGAGACTCAGAGTCCCATAGCAGGGTGCGTGAACGCAGTGAACGC GAACAACGCATGCAGGCGCAGTGGGAGCGCGAGGAGCGTGAGCGGCTGGAGATTGCCCGAGAGAGGCTGG CCTTCCAGCGCCAGCGGCTGGAGCGGGAGCGCATGGAGCGGGAACGGCTGGAGCGCGAACGCATGCACGT GGAGCACGAGCGCAGGCGCGAGCAGGAGCGCATCCACCGTGAGCGCGAGGAGCTGAGGCGCCAGCAGGAA CTGCGCTATGAGCAGGAGCGGCGGCCCGCGGTGCGGCGGCCCTACGACCTGGACCGGCGAGATGATGCCT ATTGGCCGGAAGCCAAGCGGGCCGCCCTGGATGAGCGCTACCATTCTGACTTTAACCGCCAGGACCGCTT CCACGACTTTGACCACAGGGACCGCGGCCGCTACCCCGACCACTCGGTGGACAGGAGAGAAGGTTCAAGG TCAATGATGGGAGAACGAGAAGGACAGCATTACCCAGAACGCCATGGAGGACCAGAGCGCCACGGCCGGG ACTCCCGCGATGGCTGGGGGGGCTATGGCTCTGACAAGAGGATGAGCGAGGGCCGGGGGCTGCCTCCTCC CCCCAGGGGCAGACGTGACTGGGGGGACCATGGCCGAAGAGAGGATGACCGGTCATGGCAGGGCACGGCC GACGGGGGCATGATGGACAGGGATCACAAGAGGTGGCAAGGTGGCGAGAGAAGCATGTCCGGTCACTCCG GGCCTGGCCACATGATGAACCGAGGAGGAATGTCAGGGCGCGGCAGCTTTGCCCCAGGCGGGGCCTCCCG GGGCCACCCCATCCCACACGGTGGCATGCAGGGCGGGTTTGGAGGCCAGAGCCGGGGGAGCAGGCCCAGC GATGCCCGCTTCACTCGCCGCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234961 representing NM_001201340
Red=Cloning site Green=Tags(s) MAETLSGLGDSGAAGAAALSSASSETGTRRLSDLRVIDLRAELRKRNVDSSGNKSVLMERLKKAIEDEGG NPDEIEITSEGNKKTSKRSSKGRKPEEEGVEDNGLEENSGDGQIEDKETINNLDTSSSDFTILQEIEEPS LEPENEKILDILGETCKSEPVKEESSELEQPFAQDTSSVGPDRKLAEEEDLFDSAHPEEGDLDLASESTA HAQSSKADSLLAVVKREPAEQPGDGERTDCEPVGLEPAVEQSSAASELAEASSEELAEAPTEAPSPEARD SKEDGRKFDFDACNEVPPAPKESSTSEGADQKMSSPEDDSDTKRLSKEEKGRSSCGRNFWVSGLSSTTRA TDLKNLFSKYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCINHLHKTELHGKMISVEKAKNEPVGK KTSDKRDSDGKKEKSSNSDRSTNLKRDDKCDRKDDAKKGDDGSGEKSKDQDDQKPGPSERSRATKSGSRG TERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKVKEPRKSRDSESHSRVRERSER EQRMQAQWEREERERLEIARERLAFQRQRLERERMERERLERERMHVEHERRREQERIHREREELRRQQE LRYEQERRPAVRRPYDLDRRDDAYWPEAKRAALDERYHSDFNRQDRFHDFDHRDRGRYPDHSVDRREGSR SMMGEREGQHYPERHGGPERHGRDSRDGWGGYGSDKRMSEGRGLPPPPRGRRDWGDHGRREDDRSWQGTA DGGMMDRDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHPIPHGGMQGGFGGQSRGSRPS DARFTRRY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001201340 |
| ORF Size | 2544 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001201340.2 |
| RefSeq Size | 2914 bp |
| RefSeq ORF | 2547 bp |
| Locus ID | 6294 |
| UniProt ID | Q15424 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011] |
Documents
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