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PAF1 (NM_001256826) Human Tagged ORF Clone
CAT＃: RG234246
- TrueORF®
PAF1 (tGFP-tagged) - Homo sapiens Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (PAF1), transcript variant 2
ORF Plasmid: DDK
"NM_001256826" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | F23149_1; PD2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234246 representing NM_001256826
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCCACCATCCAGACCCAGGCCCAGCGGGAGGATGGCCACAGGTCTGGAGTGGTCTGCCGAGTCA AGTACTGCAATAGCCTCCCTGATATCCCCTTCGACCCCAAGTTCATCACCTACCCCTTCGACCAGAACAG GTTCGTCCAGTACAAAGCCACTTCCTTGGAGAAACAGCACAAACATGACCTCCTGACTGAGCCAGACCTG GGGGTCACCATCGATCTCATCAATCCTGACACCTACCGCATCGACCCCAATGTTCTTCTAGATCCAGCTG ATGAGAAACTTTTGGAAGAGGAGATTCAGGCCCCCACCAGCTCCAAGAGATCCCAGCAGCACGCGAAGGT GGTGCCATGGATGCGAAAGACAGAGTACATCTCCACTGAGTTCAACCGTTATGGCATCTCCAATGAGAAG CCTGAGGTCAAGATTGGGGTTTCTGTGAAGCAGCAGTTTACCGAGGAAGAAATATACAAAGACAGGGATA GCCAGATCACAGCCATTGAGAAGACTTTTGAGGATGCCCAGAAATCAATCTCACAGCATTACAGCAAACC CCGAGTCACACCGGTGGAGGTCATGCCTGTCTTCCCAGACTTTAAGATGTGGATCAATCCATGTGCTCAG GTGATCTTTGACTCAGACCCAGCCCCCAAGGACACGAGTGGTGCAGCTGCGTTGGAGATGATGTCTCAGG CCATGATTAGGGGCATGATGGATGAGGAAGGGAACCAGTTTGTGGCCTATTTCCTGCCTGTAGAAGAGAC GTTGAAGAAACGAAAGCGGGACCAGGAGGAGGAGATGGACTATGCACCAGATGATGTGTATGACTACAAA ATTGCTCGGGAGTACAACTGGAACGTGAAGAACAAAGCTAGCAAGGGCTATGAGGAAAACTACTTCTTCA TCTTCCGAGAGGGTGACGGGGTTTACTACAATGAGTTGGAAACCAGGGTCCGCCTTAGTAAGCGCCGGGC CAAGGCTGGGGTTCAGTCAGGCACCAACGCCCTGCTTGTGGTCAAACATCGGGACATGAATGAGAAGGAA CTGGAAGCTCAGGAGGCACGGAAGGCCCAGCTAGAAAACCACGAACCGGAGGAGGAAGAGGAAGAGGAGA TGGAGACAGAAGAGAAAGAAGCTGGGGGCTCAGTGATGCTGATTCTGAGGACGATGCCGACTCTGATGAT GAGGACAGAGGACAGGCCCAAGGTGGCAGTGACAATGATTCAGACAGCGGCAGCAATGGGGGTGGCCAGC GGAGCCGGAGCCACAGCCGCAGCGCCAGTCCCTTCCCCAGTGGCAGCGAGCACTCGGCCCAGGAGGATGG CAGTGAAGCTGCAGCTTCTGATTCCAGTGAAGCTGATAGTGACAGTGACTGAGTCCCAGGGCATTCAGGG CTGGTTCAGACACCATTATTGTGAGCAGCAAAGCACTTTTCTAGTGGTCTGTTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234246 representing NM_001256826
Red=Cloning site Green=Tags(s) MAPTIQTQAQREDGHRSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDL GVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEK PEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQ VIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYK IAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKE LEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSVMLILRTMPTLMMRTEDRPKVAVTMIQTAAAMGVAS GAGATAAAPVPSPVAASTRPRRMAVKLQLLIPVKLIVTVTESQGIQGWFRHHYCEQQSTFLVVCL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001256826 |
| ORF Size | 1455 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001256826.2 |
| RefSeq Size | 2106 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 54623 |
| UniProt ID | Q8N7H5 |
| Gene Summary | This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC234246 | PAF1 (Myc-DDK tagged) - Homo sapiens Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (PAF1), transcript variant 2 |
CNY 3,990.00 |
|
| SC332485 | PAF1 (untagged) - Homo sapiens Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) (PAF1), transcript variant 2 |
CNY 4,660.00 |
