PAF1 (NM_001256826) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	F23149_1; PD2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332485 representing NM_001256826. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGCCCACCATCCAGACCCAGGCCCAGCGGGAGGATGGCCACAGGTCTGGAGTGGTCTGCCGAGTC AAGTACTGCAATAGCCTCCCTGATATCCCCTTCGACCCCAAGTTCATCACCTACCCCTTCGACCAGAAC AGGTTCGTCCAGTACAAAGCCACTTCCTTGGAGAAACAGCACAAACATGACCTCCTGACTGAGCCAGAC CTGGGGGTCACCATCGATCTCATCAATCCTGACACCTACCGCATCGACCCCAATGTTCTTCTAGATCCA GCTGATGAGAAACTTTTGGAAGAGGAGATTCAGGCCCCCACCAGCTCCAAGAGATCCCAGCAGCACGCG AAGGTGGTGCCATGGATGCGAAAGACAGAGTACATCTCCACTGAGTTCAACCGTTATGGCATCTCCAAT GAGAAGCCTGAGGTCAAGATTGGGGTTTCTGTGAAGCAGCAGTTTACCGAGGAAGAAATATACAAAGAC AGGGATAGCCAGATCACAGCCATTGAGAAGACTTTTGAGGATGCCCAGAAATCAATCTCACAGCATTAC AGCAAACCCCGAGTCACACCGGTGGAGGTCATGCCTGTCTTCCCAGACTTTAAGATGTGGATCAATCCA TGTGCTCAGGTGATCTTTGACTCAGACCCAGCCCCCAAGGACACGAGTGGTGCAGCTGCGTTGGAGATG ATGTCTCAGGCCATGATTAGGGGCATGATGGATGAGGAAGGGAACCAGTTTGTGGCCTATTTCCTGCCT GTAGAAGAGACGTTGAAGAAACGAAAGCGGGACCAGGAGGAGGAGATGGACTATGCACCAGATGATGTG TATGACTACAAAATTGCTCGGGAGTACAACTGGAACGTGAAGAACAAAGCTAGCAAGGGCTATGAGGAA AACTACTTCTTCATCTTCCGAGAGGGTGACGGGGTTTACTACAATGAGTTGGAAACCAGGGTCCGCCTT AGTAAGCGCCGGGCCAAGGCTGGGGTTCAGTCAGGCACCAACGCCCTGCTTGTGGTCAAACATCGGGAC ATGAATGAGAAGGAACTGGAAGCTCAGGAGGCACGGAAGGCCCAGCTAGAAAACCACGAACCGGAGGAG GAAGAGGAAGAGGAGATGGAGACAGAAGAGAAAGAAGCTGGGGGCTCAGTGATGCTGATTCTGAGGACG ATGCCGACTCTGATGATGAGGACAGAGGACAGGCCCAAGGTGGCAGTGACAATGATTCAGACAGCGGCA GCAATGGGGGTGGCCAGCGGAGCCGGAGCCACAGCCGCAGCGCCAGTCCCTTCCCCAGTGGCAGCGAGC ACTCGGCCCAGGAGGATGGCAGTGAAGCTGCAGCTTCTGATTCCAGTGAAGCTGATAGTGACAGTGACT GAGTCCCAGGGCATTCAGGGCTGGTTCAGACACCATTATTGTGAGCAGCAAAGCACTTTTCTAGTGGTC TGTTTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256826
Insert Size	1458 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256826.1
RefSeq Size	2106 bp
RefSeq ORF	1458 bp
Locus ID	54623
UniProt ID	Q8N7H5
MW	55.5 kDa
Gene Summary	This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region and uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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