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HEF1 (NEDD9) (NM_001271033) Human Tagged ORF Clone
CAT＃: RG233078
- TrueORF®
NEDD9 (tGFP-tagged) - Homo sapiens neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 4
ORF Plasmid: DDK
"NM_001271033" in other vectors (2)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAS-L; CAS2; CASL; CASS2; HEF1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG233078 representing NM_001271033
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTATAAGGTGATAACCCCCGTGAGGACAGGCCATGGCTACGTATACGAGTACCCATCCAGATACC AAAAGGACGTCTATGATATCCCTCCTTCTCATACCACTCAAGGGGTATACGACATCCCTCCCTCATCAGC AAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAGATAAAACCTCAAGGGGTGTATGACATCCCGCCTACA AAAGGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAGCAGGGCTTAGGGAAAAAGACTATGACT TCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACCGGAGGGGGTTTATGACATTCCTCCAAC CTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAACTGTGACATTCCAGGAGCTGCAGAACCG GTGGCTCGAAGGCACCAGAGCCTGTCCCCGAATCACCCACCCCCGCAACTCGGACAGTCAGTGGGCTCTC AGAACGACGCATATGATGTCCCCCGAGGCGTTCAGTTTCTTGAGCCACCAGCAGAAACCAGTGAGAAAGC AAACCCCCAGGAAAGGGATGGTGTTTATGATGTCCCTCTGCATAACCCGCCAGATGCTAAAGGCTCTCGG GACTTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAGGCAGCACCCGGAGTAACATGTCCACGT CTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGCTCAGGACAAAAGGCTCTTCCTGGATCC AGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTTGAGATGGGTGTCTCCAGCCTAATGGCA CTGGTCACTACCGACTGGCGGTGTTACGGATATATGGAAAGACACATCAATGAAATACGCACAGCAGTGG ACAAGGTGGAGCTGTTCCTGAAGGAGTACCTCCACTTTGTCAAGGGAGCTGTTGCAAATGCTGCCTGCCT CCCGGAACTCATCCTCCACAACAAGATGAAGCGGGAGCTGCAACGAGTTGAAGACTCCCACCAGATCCTG AGTCAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGAATATCTTGGCCATCAACAAGCCCCAGA ACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGACGGTGCCCGATGACGCCAAGCAGCTCAC CACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGCCCTGGCAGCTTGCATCTGAAGAATGGG CCGGAGAGCATCATGAACTCAACGGAGTACCCACACGGTGGCTCCCAGGGACAGCTGCTGCATCCTGGTG ACCACAAGGCCCAGGCCCACAACAAGGCACTGCCCCCAGGCCTGAGCAAGGAGCAGGCCCCTGACTGTAG CAGCAGTGATGGTTCTGAGAGGAGCTGGATGGATGACTACGATTACGTCCACCTACAGGGTAAGGAGGAG TTTGAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCATGAAACAGAACAAGATGCAGCTGGAAC ATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTACAAAGCCCGTGGAGAATGACATCTCGAA GTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTGAGTGCTCAGGATCGGCAGTTGCTGTGC TTCTACTATGACCAATGTGAGACCCATTTCATTTCCCTTCTCAACGCCATTGACGCACTCTTCAGTTGTG TCAGCTCAGCCCAGCCCCCGCGAATCTTCGTGGCACACAGCAAGTTTGTCATCCTCAGTGCACACAAACT GGTGTTCATTGGAGACACGCTGACACGGCAGGTGACTGCCCAGGACATTCGCAACAAAGTCATGAACTCC AGCAACCAGCTCTGCGAGCAGCTCAAGACCATAGTCATGGCAACCAAGATGGCCGCCCTCCATTACCCCA GCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGACCTTTCTAGAAATGCCCAGCTGTTCAAGCG CTCTTTGCTGGAGATGGCAACGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233078 representing NM_001271033
Red=Cloning site Green=Tags(s) MKYKVITPVRTGHGYVYEYPSRYQKDVYDIPPSHTTQGVYDIPPSSAKGPVFSVPVGEIKPQGVYDIPPT KGVYAIPPSACRDEAGLREKDYDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYNCDIPGAAEP VARRHQSLSPNHPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLHNPPDAKGSR DLVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQALEMGVSSLMA LVTTDWRCYGYMERHINEIRTAVDKVELFLKEYLHFVKGAVANAACLPELILHNKMKRELQRVEDSHQIL SQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPDDAKQLTTTINTNAEALFRPGPGSLHLKNG PESIMNSTEYPHGGSQGQLLHPGDHKAQAHNKALPPGLSKEQAPDCSSSDGSERSWMDDYDYVHLQGKEE FERQQKELLEKENIMKQNKMQLEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNSGVSAQDRQLLC FYYDQCETHFISLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVTAQDIRNKVMNS SNQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001271033 |
| ORF Size | 2055 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001271033.1, NP_001257962.1 |
| RefSeq Size | 4103 bp |
| RefSeq ORF | 2058 bp |
| Locus ID | 4739 |
| UniProt ID | Q14511 |
| Gene Summary | The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC233078 | NEDD9 (Myc-DDK tagged) - Homo sapiens neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 4 |
CNY 5,800.00 |
|
| SC330917 | NEDD9 (untagged) - Homo sapiens neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 4 |
CNY 6,650.00 |
