HEF1 (NEDD9) (NM_001271033) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAS-L; CAS2; CASL; CASS2; HEF1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330917 representing NM_001271033. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGTATAAGGTGATAACCCCCGTGAGGACAGGCCATGGCTACGTATACGAGTACCCATCCAGATAC CAAAAGGACGTCTATGATATCCCTCCTTCTCATACCACTCAAGGGGTATACGACATCCCTCCCTCATCA GCAAAAGGCCCTGTGTTTTCAGTTCCAGTGGGAGAGATAAAACCTCAAGGGGTGTATGACATCCCGCCT ACAAAAGGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAGCAGGGCTTAGGGAAAAAGACTAT GACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACCGGAGGGGGTTTATGACATTCCT CCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAACTGTGACATTCCAGGAGCTGCA GAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCGAATCACCCACCCCCGCAACTCGGACAGTCAGTG GGCTCTCAGAACGACGCATATGATGTCCCCCGAGGCGTTCAGTTTCTTGAGCCACCAGCAGAAACCAGT GAGAAAGCAAACCCCCAGGAAAGGGATGGTGTTTATGATGTCCCTCTGCATAACCCGCCAGATGCTAAA GGCTCTCGGGACTTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAGGCAGCACCCGGAGTAAC ATGTCCACGTCTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGCTCAGGACAAAAGGCTC TTCCTGGATCCAGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTTGAGATGGGTGTCTCC AGCCTAATGGCACTGGTCACTACCGACTGGCGGTGTTACGGATATATGGAAAGACACATCAATGAAATA CGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAGGAGTACCTCCACTTTGTCAAGGGAGCTGTTGCA AATGCTGCCTGCCTCCCGGAACTCATCCTCCACAACAAGATGAAGCGGGAGCTGCAACGAGTTGAAGAC TCCCACCAGATCCTGAGTCAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGAATATCTTGGCC ATCAACAAGCCCCAGAACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGACGGTGCCCGAT GACGCCAAGCAGCTCACCACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGCCCTGGCAGC TTGCATCTGAAGAATGGGCCGGAGAGCATCATGAACTCAACGGAGTACCCACACGGTGGCTCCCAGGGA CAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCCCACAACAAGGCACTGCCCCCAGGCCTGAGCAAG GAGCAGGCCCCTGACTGTAGCAGCAGTGATGGTTCTGAGAGGAGCTGGATGGATGACTACGATTACGTC CACCTACAGGGTAAGGAGGAGTTTGAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCATGAAA CAGAACAAGATGCAGCTGGAACATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTACAAAG CCCGTGGAGAATGACATCTCGAAGTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTGAGT GCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGACCAATGTGAGACCCATTTCATTTCCCTTCTCAAC GCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCCCAGCCCCCGCGAATCTTCGTGGCACACAGCAAG TTTGTCATCCTCAGTGCACACAAACTGGTGTTCATTGGAGACACGCTGACACGGCAGGTGACTGCCCAG GACATTCGCAACAAAGTCATGAACTCCAGCAACCAGCTCTGCGAGCAGCTCAAGACCATAGTCATGGCA ACCAAGATGGCCGCCCTCCATTACCCCAGCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGAC CTTTCTAGAAATGCCCAGCTGTTCAAGCGCTCTTTGCTGGAGATGGCAACGTTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001271033
Insert Size	2058 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271033.1
RefSeq Size	4103 bp
RefSeq ORF	2058 bp
Locus ID	4739
UniProt ID	Q14511
MW	76.6 kDa
Gene Summary	The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (4) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 4 which is shorter than isoform 1.

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