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GPR161 (NM_001267612) Human Tagged ORF Clone
CAT＃: RG232654
- TrueORF®
GPR161 (tGFP-tagged) - Homo sapiens G protein-coupled receptor 161 (GPR161), transcript variant 5
ORF Plasmid: DDK
"NM_001267612" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RE2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG232654 representing NM_001267612
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTACCCCATGAAGATCACAGGGAACCGGGCTGTGATGGCACTTGTCTACATCTGGCTTCACTCGC TCATCGGCTGCCTGCCACCCCTGTTTGGTTGGTCATCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGT GGCTGCTTGGCACCGGGAGCCTGGCTACACGGCCTTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTTCTG GTCATGCTGGTGTGCTATGGCTTCATCTTCCGCGTGGCCAGGGTCAAGGCACGCAAGGTGCACTGTGGCA CAGTCGTCATCGTGGAGGAGGATGCTCAGAGGACCGGGAGGAAGAACTCCAGCACCTCCACCTCCTCTTC AGGCAGCAGGAGGAATGCCTTTCAGGGTGTGGTCTACTCGGCCAACCAGTGCAAAGCCCTCATCACCATC CTGGTGGTCCTCGGTGCCTTCATGGTCACCTGGGGCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCT GGGGGAAAAGCTCCGTCTCCCCGAGCCTGGAGACTTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTG CCACCCCCTGATCTATGGACTCTGGAACAAGACAGTTCGCAAAGAACTACTGGGCATGTGCTTTGGGGAC CGGTATTATCGGGAACCATTTGTGCAACGACAGAGGACTTCCAGGCTCTTCAGCATTTCCAACAGGATCA CAGACCTGGGCCTGTCCCCACACCTCACTGCGCTCATGGCAGGTGGACAGCCCCTGGGGCACAGCAGCAG CACGGGGGACACTGGCTTCAGCTGCTCCCAGGACTCAGGGACAGATATGATGCTGCTTGAGGACTACACG TCTGATGACAACCCTCCCTCTCACTGCACTTGCCCACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATG AAGTGGAACAAATCAAAGAAGCTGCCAAGAACTCGATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTT GGACAGTTACGCAGCAAGCTTGGCCAAAGCCATTGAGGCCGAAGCCAAAATCAACTTATTTGGGGAGGAG GCTTTGCCAGGGGTCTTGGTTACAGCACGGACTGTCCCGGGGGGCGGCTTCGGGGGCCGCCGAGGCAGCA GAACTCTTGTGAGCCAGAGGCTGCAGTTGCAGAGCATCGAAGAAGGAGATGTTTTAGCTGCCGAGCAGAG A ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG232654 representing NM_001267612
Red=Cloning site Green=Tags(s) MVYPMKITGNRAVMALVYIWLHSLIGCLPPLFGWSSVEFDEFKWMCVAAWHREPGYTAFWQIWCALFPFL VMLVCYGFIFRVARVKARKVHCGTVVIVEEDAQRTGRKNSSTSTSSSGSRRNAFQGVVYSANQCKALITI LVVLGAFMVTWGPYMVVIASEALWGKSSVSPSLETWATWLSFASAVCHPLIYGLWNKTVRKELLGMCFGD RYYREPFVQRQRTSRLFSISNRITDLGLSPHLTALMAGGQPLGHSSSTGDTGFSCSQDSGTDMMLLEDYT SDDNPPSHCTCPPKRRSSVTFEDEVEQIKEAAKNSILHVKAEVHKSLDSYAASLAKAIEAEAKINLFGEE ALPGVLVTARTVPGGGFGGRRGSRTLVSQRLQLQSIEEGDVLAAEQR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001267612 |
| ORF Size | 1191 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001267612.2 |
| RefSeq Size | 7771 bp |
| RefSeq ORF | 1194 bp |
| Locus ID | 23432 |
| UniProt ID | Q8N6U8 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017] |
Documents
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| SDS |
