GPR161 (NM_001267612) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RE2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330770 representing NM_001267612. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTGTACCCCATGAAGATCACAGGGAACCGGGCTGTGATGGCACTTGTCTACATCTGGCTTCACTCG CTCATCGGCTGCCTGCCACCCCTGTTTGGTTGGTCATCCGTGGAGTTTGACGAGTTCAAATGGATGTGT GTGGCTGCTTGGCACCGGGAGCCTGGCTACACGGCCTTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTT CTGGTCATGCTGGTGTGCTATGGCTTCATCTTCCGCGTGGCCAGGGTCAAGGCACGCAAGGTGCACTGT GGCACAGTCGTCATCGTGGAGGAGGATGCTCAGAGGACCGGGAGGAAGAACTCCAGCACCTCCACCTCC TCTTCAGGCAGCAGGAGGAATGCCTTTCAGGGTGTGGTCTACTCGGCCAACCAGTGCAAAGCCCTCATC ACCATCCTGGTGGTCCTCGGTGCCTTCATGGTCACCTGGGGCCCCTACATGGTTGTCATCGCCTCTGAG GCCCTCTGGGGGAAAAGCTCCGTCTCCCCGAGCCTGGAGACTTGGGCCACATGGCTGTCCTTTGCCAGC GCTGTCTGCCACCCCCTGATCTATGGACTCTGGAACAAGACAGTTCGCAAAGAACTACTGGGCATGTGC TTTGGGGACCGGTATTATCGGGAACCATTTGTGCAACGACAGAGGACTTCCAGGCTCTTCAGCATTTCC AACAGGATCACAGACCTGGGCCTGTCCCCACACCTCACTGCGCTCATGGCAGGTGGACAGCCCCTGGGG CACAGCAGCAGCACGGGGGACACTGGCTTCAGCTGCTCCCAGGACTCAGGGACAGATATGATGCTGCTT GAGGACTACACGTCTGATGACAACCCTCCCTCTCACTGCACTTGCCCACCCAAGAGAAGGAGCTCGGTG ACATTTGAGGATGAAGTGGAACAAATCAAAGAAGCTGCCAAGAACTCGATTCTTCATGTGAAAGCTGAA GTACACAAGTCCTTGGACAGTTACGCAGCAAGCTTGGCCAAAGCCATTGAGGCCGAAGCCAAAATCAAC TTATTTGGGGAGGAGGCTTTGCCAGGGGTCTTGGTTACAGCACGGACTGTCCCGGGGGGCGGCTTCGGG GGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGGCTGCAGTTGCAGAGCATCGAAGAAGGAGATGTT TTAGCTGCCGAGCAGAGATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267612
Insert Size	1194 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267612.1
RefSeq Size	7771 bp
RefSeq ORF	1194 bp
Locus ID	23432
UniProt ID	Q8N6U8
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	43.9 kDa
Gene Summary	The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (5) differs in the 5' UTR and lacks a portion of the 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (4) with a shorter N-terminus compared to isoform 1. Variants 5 and 11 both encode the same isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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