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cPLA2 beta (PLA2G4B) (NM_001114633) Human Tagged ORF Clone
CAT＃: RG226162
- TrueORF®
PLA2G4B (tGFP-tagged) - Human phospholipase A2, group IVB (cytosolic) (PLA2G4B)
ORF Plasmid: DDK
"NM_001114633" in other vectors (6)
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CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | cPLA2-beta; HsT16992 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226162 representing NM_001114633
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGGCAGAGGTGTCCAGGACCTGCCTGCTCACGGTTCGTGTCCTGCAGGCCCATCGCCTACCCT CTAAGGACCTAGTGACCCCCTCTGACTGCTACGTGACTCTCTGGCTGCCCACGGCCTGCAGCCACAGGCT CCAGACACGCACGGTCAAGAACAGCAGTAGCCCTGTCTGGAACCAGAGCTTTCACTTCAGGATCCACAGG CAGCTCAAGAATGTCATGGAACTGAAAGTCTTTGACCAGGACCTGGTGACCGGAGATGACCCTGTGTTGT CAGTACTGTTTGATGCGGGGACTCTGCGGGCTGGGGAGTTCCGGCGCGAGAGCTTCTCACTGAGCCCTCA GGGTGAGGGGCGCCTGGAAGTTGAATTTCGCCTGCAGAGTCTGGCTGACCGTGGCGAGTGGCTCGTCAGC AATGGCGTTCTGGTGGCCCGGGAGCTCTCCTGCTTGCACGTTCAACTGGAGGAGACAGGAGACCAGAAGT CCTCAGAGCACAGAGTTCAGCTTGTGGTTCCTGGGTCCTGTGAGGGTCCGCAGGAGGCCTCTGTGGGCAC TGGCACCTTCCGCTTCCACTGCCCAGCCTGCTGGGAGCAGGAGCTGAGTATTCGCCTGCAGGATGCCCCC GAGGAGCAACTAAAGGCGCCACTGAGTGCCCTGCCCTCTGGTCAAGTGGTGAGGCTTGTCTTCCCCACGT CCCAGGAGCCCCTGATGAGAGTGGAGCTGAAAAAAGAAGCAGGACTGAGGGAGCTGGCCGTGCGACTGGG CTTCGGGCCCTGTGCAGAGGAGCAGGCCTTCCTGAGCAGGAGGAAGCAGGTGGTGGCCGCGGCCTTGAGG CAGGCCCTGCAGCTGGACGGAGACCTGCAGGAGGATGAGATCCCAGTGGTAGCTATTATGGCCACTGGTG GTGGGATCCGGGCAATGACTTCCCTGTATGGGCAGCTGGCTGGCCTGAAGGAGCTGGGCCTCTTGGATTG CGTCTCCTACATCACCGGGGCCTCGGGCTCCACCTGGGCCTTGGCCAACCTTTATGAGGACCCAGAGTGG TCTCAGAAGGACCTGGCAGGGCCCACTGAGTTGCTGAAGACCCAGGTGACCAAGAACAAGCTGGGTGTGC TGGCCCCCAGCCAGCTGCAGCGGTACCGGCAGGAGCTGGCCGAGCGTGCCCGCTTGGGCTACCCAAGCTG CTTCACCAACCTGTGGGCCCTCATCAACGAGGCGCTGCTGCATGATGAGCCCCATGATCACAAGCTCTCA GATCAACGGGAGGCCCTGAGTCATGGCCAGAACCCTCTGCCCATCTACTGTGCCCTCAACACCAAAGGGC AGAGCCTGACCACTTTTGAATTTGGGGAGTGGTGCGAGTTCTCTCCCTACGAGGTCGGCTTCCCCAAGTA CGGGGCCTTCATCCCCTCTGAGCTCTTTGGCTCCGAGTTCTTTATGGGGCAGCTGATGAAGAGGCTTCCT GAGTCCCGCATCTGCTTCTTAGAAGGTATCTGGAGCAACCTGTATGCAGCCAACCTCCAGGACAGCTTAT ACTGGGCCTCAGAGCCCAGCCAGTTCTGGGACCGCTGGGTCAGGAACCAGGCCAACCTGGACAAGGAGCA GGTCCCCCTTCTGAAGATAGAAGAACCACCCTCAACAGCCGGCAGGATAGCTGAGTTTTTCACCGATCTT CTGACGTGGCGTCCACTGGCCCAGGCCACACATAATTTCCTGCGTGGCCTCCATTTCCACAAAGACTACT TTCAGCATCCTCACTTCTCCACATGGAAAGCTACCACTCTGGATGGGCTCCCCAACCAGCTGACACCCTC GGAGCCCCACCTGTGCCTGCTGGATGTTGGCTACCTCATCAATACCAGCTGCCTGCCCCTCCTGCAGCCC ACTCGGGACGTGGACCTCATCCTGTCATTGGACTACAACCTCCACGGAGCCTTCCAGCAGTTGCAGCTCC TGGGCCGGTTCTGCCAGGAGCAGGGGATCCCGTTCCCACCCATCTCGCCCAGCCCCGAAGAGCAGCTCCA GCCTCGGGAGTGCCACACCTTCTCCGACCCCACCTGCCCCGGAGCCCCTGCGGTGCTGCACTTTCCTCTG GTCAGCGACTCCTTCCGGGAGTACTCGGCCCCTGGGGTCCGGCGGACACCCGAGGAGGCGGCAGCTGGGG AGGTGAACCTGTCTTCATCGGACTCTCCCTACCACTACACGAAGGTGACCTACAGCCAGGAGGACGTGGA CAAGCTGCTGCACCTGACACATTACAATGTCTGCAACAACCAGGAGCAGCTGCTGGAGGCTCTGCGCCAG GCAGTGCAGCGGAGGCGGCAGCGCAGGCCCCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226162 representing NM_001114633
Red=Cloning site Green=Tags(s) MAVAEVSRTCLLTVRVLQAHRLPSKDLVTPSDCYVTLWLPTACSHRLQTRTVKNSSSPVWNQSFHFRIHR QLKNVMELKVFDQDLVTGDDPVLSVLFDAGTLRAGEFRRESFSLSPQGEGRLEVEFRLQSLADRGEWLVS NGVLVARELSCLHVQLEETGDQKSSEHRVQLVVPGSCEGPQEASVGTGTFRFHCPACWEQELSIRLQDAP EEQLKAPLSALPSGQVVRLVFPTSQEPLMRVELKKEAGLRELAVRLGFGPCAEEQAFLSRRKQVVAAALR QALQLDGDLQEDEIPVVAIMATGGGIRAMTSLYGQLAGLKELGLLDCVSYITGASGSTWALANLYEDPEW SQKDLAGPTELLKTQVTKNKLGVLAPSQLQRYRQELAERARLGYPSCFTNLWALINEALLHDEPHDHKLS DQREALSHGQNPLPIYCALNTKGQSLTTFEFGEWCEFSPYEVGFPKYGAFIPSELFGSEFFMGQLMKRLP ESRICFLEGIWSNLYAANLQDSLYWASEPSQFWDRWVRNQANLDKEQVPLLKIEEPPSTAGRIAEFFTDL LTWRPLAQATHNFLRGLHFHKDYFQHPHFSTWKATTLDGLPNQLTPSEPHLCLLDVGYLINTSCLPLLQP TRDVDLILSLDYNLHGAFQQLQLLGRFCQEQGIPFPPISPSPEEQLQPRECHTFSDPTCPGAPAVLHFPL VSDSFREYSAPGVRRTPEEAAAGEVNLSSSDSPYHYTKVTYSQEDVDKLLHLTHYNVCNNQEQLLEALRQ AVQRRRQRRPH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001114633 |
| ORF Size | 2343 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001114633.1, NP_001108105.1 |
| RefSeq Size | 2752 bp |
| RefSeq ORF | 2346 bp |
| Locus ID | 100137049 |
| UniProt ID | P0C869 |
| Gene Summary | This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226162 | PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 8,576.00 |
|
| RC226162L1 | Lenti-ORF clone of PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,976.00 |
|
| RC226162L2 | Lenti-ORF clone of PLA2G4B (mGFP-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,170.00 |
|
| RC226162L3 | Lenti-ORF clone of PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,170.00 |
|
| RC226162L4 | Lenti-ORF clone of PLA2G4B (mGFP-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,170.00 |
|
| SC318981 | PLA2G4B (untagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 15,110.00 |
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