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cPLA2 beta (PLA2G4B) (NM_001114633) Human Untagged Clone
CAT＃: SC318981
PLA2G4B (untagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B)
CNY 15,110.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | cPLA2-beta; HsT16992 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318981 representing NM_001114633.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGTGGCAGAGGTGTCCAGGACCTGCCTGCTCACGGTTCGTGTCCTGCAGGCCCATCGCCTACCC TCTAAGGACCTAGTGACCCCCTCTGACTGCTACGTGACTCTCTGGCTGCCCACGGCCTGCAGCCACAGG CTCCAGACACGCACGGTCAAGAACAGCAGTAGCCCTGTCTGGAACCAGAGCTTTCACTTCAGGATCCAC AGGCAGCTCAAGAATGTCATGGAACTGAAAGTCTTTGACCAGGACCTGGTGACCGGAGATGACCCTGTG TTGTCAGTACTGTTTGATGCGGGGACTCTGCGGGCTGGGGAGTTCCGGCGCGAGAGCTTCTCACTGAGC CCTCAGGGTGAGGGGCGCCTGGAAGTTGAATTTCGCCTGCAGAGTCTGGCTGACCGTGGCGAGTGGCTC GTCAGCAATGGCGTTCTGGTGGCCCGGGAGCTCTCCTGCTTGCACGTTCAACTGGAGGAGACAGGAGAC CAGAAGTCCTCAGAGCACAGAGTTCAGCTTGTGGTTCCTGGGTCCTGTGAGGGTCCGCAGGAGGCCTCT GTGGGCACTGGCACCTTCCGCTTCCACTGCCCAGCCTGCTGGGAGCAGGAGCTGAGTATTCGCCTGCAG GATGCCCCCGAGGAGCAACTAAAGGCGCCACTGAGTGCCCTGCCCTCTGGTCAAGTGGTGAGGCTTGTC TTCCCCACGTCCCAGGAGCCCCTGATGAGAGTGGAGCTGAAAAAAGAAGCAGGACTGAGGGAGCTGGCC GTGCGACTGGGCTTCGGGCCCTGTGCAGAGGAGCAGGCCTTCCTGAGCAGGAGGAAGCAGGTGGTGGCC GCGGCCTTGAGGCAGGCCCTGCAGCTGGACGGAGACCTGCAGGAGGATGAGATCCCAGTGGTAGCTATT ATGGCCACTGGTGGTGGGATCCGGGCAATGACTTCCCTGTATGGGCAGCTGGCTGGCCTGAAGGAGCTG GGCCTCTTGGATTGCGTCTCCTACATCACCGGGGCCTCGGGCTCCACCTGGGCCTTGGCCAACCTTTAT GAGGACCCAGAGTGGTCTCAGAAGGACCTGGCAGGGCCCACTGAGTTGCTGAAGACCCAGGTGACCAAG AACAAGCTGGGTGTGCTGGCCCCCAGCCAGCTGCAGCGGTACCGGCAGGAGCTGGCCGAGCGTGCCCGC TTGGGCTACCCAAGCTGCTTCACCAACCTGTGGGCCCTCATCAACGAGGCGCTGCTGCATGATGAGCCC CATGATCACAAGCTCTCAGATCAACGGGAGGCCCTGAGTCATGGCCAGAACCCTCTGCCCATCTACTGT GCCCTCAACACCAAAGGGCAGAGCCTGACCACTTTTGAATTTGGGGAGTGGTGCGAGTTCTCTCCCTAC GAGGTCGGCTTCCCCAAGTACGGGGCCTTCATCCCCTCTGAGCTCTTTGGCTCCGAGTTCTTTATGGGG CAGCTGATGAAGAGGCTTCCTGAGTCCCGCATCTGCTTCTTAGAAGGTATCTGGAGCAACCTGTATGCA GCCAACCTCCAGGACAGCTTATACTGGGCCTCAGAGCCCAGCCAGTTCTGGGACCGCTGGGTCAGGAAC CAGGCCAACCTGGACAAGGAGCAGGTCCCCCTTCTGAAGATAGAAGAACCACCCTCAACAGCCGGCAGG ATAGCTGAGTTTTTCACCGATCTTCTGACGTGGCGTCCACTGGCCCAGGCCACACATAATTTCCTGCGT GGCCTCCATTTCCACAAAGACTACTTTCAGCATCCTCACTTCTCCACATGGAAAGCTACCACTCTGGAT GGGCTCCCCAACCAGCTGACACCCTCGGAGCCCCACCTGTGCCTGCTGGATGTTGGCTACCTCATCAAT ACCAGCTGCCTGCCCCTCCTGCAGCCCACTCGGGACGTGGACCTCATCCTGTCATTGGACTACAACCTC CACGGAGCCTTCCAGCAGTTGCAGCTCCTGGGCCGGTTCTGCCAGGAGCAGGGGATCCCGTTCCCACCC ATCTCGCCCAGCCCCGAAGAGCAGCTCCAGCCTCGGGAGTGCCACACCTTCTCCGACCCCACCTGCCCC GGAGCCCCTGCGGTGCTGCACTTTCCTCTGGTCAGCGACTCCTTCCGGGAGTACTCGGCCCCTGGGGTC CGGCGGACACCCGAGGAGGCGGCAGCTGGGGAGGTGAACCTGTCTTCATCGGACTCTCCCTACCACTAC ACGAAGGTGACCTACAGCCAGGAGGACGTGGACAAGCTGCTGCACCTGACACATTACAATGTCTGCAAC AACCAGGAGCAGCTGCTGGAGGCTCTGCGCCAGGCAGTGCAGCGGAGGCGGCAGCGCAGGCCCCACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001114633 |
| Insert Size | 2346 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001114633.1 |
| RefSeq Size | 2752 bp |
| RefSeq ORF | 2346 bp |
| Locus ID | 100137049 |
| UniProt ID | P0C869 |
| MW | 88 kDa |
| Gene Summary | This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226162 | PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 8,576.00 |
|
| RC226162L1 | Lenti-ORF clone of PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,976.00 |
|
| RC226162L2 | Lenti-ORF clone of PLA2G4B (mGFP-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,170.00 |
|
| RC226162L3 | Lenti-ORF clone of PLA2G4B (Myc-DDK-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,170.00 |
|
| RC226162L4 | Lenti-ORF clone of PLA2G4B (mGFP-tagged)-Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 10,170.00 |
|
| RG226162 | PLA2G4B (tGFP-tagged) - Human phospholipase A2, group IVB (cytosolic) (PLA2G4B) |
CNY 9,120.00 |
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