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C19orf46 (SYNE4) (NM_001039876) Human Tagged ORF Clone
CAT＃: RG223193
- TrueORF®
SYNE4 (tGFP-tagged) - Human chromosome 19 open reading frame 46 (C19orf46)
ORF Plasmid: DDK
"NM_001039876" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C19orf46; DFNB76; KASH4; Nesp4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223193 representing NM_001039876
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCTGTCCCTGCCTCTGGGCCCTAGACTTGGCTCAGAGCCCCTCAACCACCCACCGGGAGCACCTA GAGAGGCGGACATTGTTGGATGCACCGTCTGCCCCGCGTCCGGAGAGGAGAGCACGAGCCCAGAGCAGGC CCAGACCCTGGGACAGGACTCCTTGGGCCCTCCTGAGCACTTCCAGGGTGGGCCAAGGGGCAATGAGCCT GCCGCTCACCCCCCGAGATGGTCAACACCCTCTTCCTACGAGGACCCAGCTGGGGGCAAACACTGTGAGC ACCCCATTTCTGGCCTGGAGGTACTAGAGGCTGAGCAGAACAGCCTGCACCTGTGCCTGCTGGGGCTGGG CCGCCGGCTGCAGGACCTGGAGCAAGGCCTGGGGCACTGGGCATTGGCCCAGAGTGGGATGGTGCAGCTG CAGGCCCTCCAGGTGGACCTACGAGGGGCAGCTGAGCGTGTGGAGGCGCTGCTAGCGTTTGGTGAGGGGC TGGCACAGCGGAGTGAGCCCAGGGCCTGGGCAGCCCTGGAGCAGATCCTGCGGGCCCTGGGAGCTTACCG AGACTCCATCTTCCGGCGGCTCTGGCAGCTGCAGGCCCAGCTGGTCAGCTACAGCCTGGTGTTCGAGGAG GCCAACACGCTGGACCAGGACTTGGAGGTCGAGGGAGACTCGGACTGGCCAGGACCTGGTGGGGTCTGGG GGCCCTGGGCACCCAGTAGCCTCCCCACTTCCACAGAGTTGGAGTGGGATCCGGCGGGGGACATTGGGGG CCTTGGGCCCTTGGGACAAAAGACAGCCCGGACACTAGGAGTGCCCTGTGAGCTGTGTGGCCAGAGGGGG CCCCAGGGCAGGGGACAAGGCCTTGAGGAAGCAGACACCTCTCACTCCCGACAGGACATGCTGGAGTCTG GCCTCGGCCACCAGAAACGCTTAGCACGTCACCAAAGACACTCCCTGCTCCGGAAGCCTCAGGACAAGAA GAGGCAAGCATCTCCTCATCTCCAGGATGTGAGGCTGGAGGGGAATCCAGGGGCCCCCGATCCTGCATCC AGGCAGCCTCTGACCTTCCTCCTTATCCTCTTCCTCCTCTTCCTCCTCCTGGTGGGTGCCATGTTTCTCC TGCCCGCGTCAGGAGGCCCCTGCTGCTCTCATGCCCGAATACCCAGGACACCCTACCTGGTGCTCAGCTA TGTCAATGGTCTTCCCCCAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223193 representing NM_001039876
Red=Cloning site Green=Tags(s) MALSLPLGPRLGSEPLNHPPGAPREADIVGCTVCPASGEESTSPEQAQTLGQDSLGPPEHFQGGPRGNEP AAHPPRWSTPSSYEDPAGGKHCEHPISGLEVLEAEQNSLHLCLLGLGRRLQDLEQGLGHWALAQSGMVQL QALQVDLRGAAERVEALLAFGEGLAQRSEPRAWAALEQILRALGAYRDSIFRRLWQLQAQLVSYSLVFEE ANTLDQDLEVEGDSDWPGPGGVWGPWAPSSLPTSTELEWDPAGDIGGLGPLGQKTARTLGVPCELCGQRG PQGRGQGLEEADTSHSRQDMLESGLGHQKRLARHQRHSLLRKPQDKKRQASPHLQDVRLEGNPGAPDPAS RQPLTFLLILFLLFLLLVGAMFLLPASGGPCCSHARIPRTPYLVLSYVNGLPPV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039876 |
| ORF Size | 1212 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039876.3 |
| RefSeq Size | 1541 bp |
| RefSeq ORF | 1215 bp |
| Locus ID | 163183 |
| UniProt ID | Q8N205 |
| Protein Families | Transmembrane |
| Gene Summary | This gene is a member of the nesprin family of genes, that encode KASH (Klarsicht, Anc-1, Syne Homology) domain-containing proteins. In addition to the KASH domain, this protein also contains a coiled-coil and leucine zipper region, a spectrin repeat, and a kinesin-1 binding region. This protein localizes to the outer nuclear membrane, and is part of the linker of nucleoskeleton and cytoskeleton (LINC) complex in the nuclear envelope. LINC complexes are formed by SUN (Sad1, UNC-84)-KASH pairs, and are thought to mechanically couple nuclear components to the cytoskeleton. Mutations in this gene have been associated with progressive high-frequency hearing loss. The absence of this protein in mice also caused hearing loss, and changes in hair cell morphology in the ears. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223193 | SYNE4 (Myc-DDK-tagged)-Human chromosome 19 open reading frame 46 (C19orf46) |
CNY 3,656.00 |
|
| RC223193L3 | Lenti ORF clone of Human chromosome 19 open reading frame 46 (C19orf46), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC223193L4 | Lenti ORF clone of Human chromosome 19 open reading frame 46 (C19orf46), mGFP tagged |
CNY 5,890.00 |
|
| SC311016 | SYNE4 (untagged)-Human chromosome 19 open reading frame 46 (C19orf46) |
CNY 6,560.00 |
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