GRIK2 (NM_021956) Human Tagged ORF Clone
CAT#: RG222369
- TrueORF®
GRIK2 (tGFP-tagged) - Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1
ORF Plasmid: DDK
"NM_021956" in other vectors (6)
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CNY 8,248.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | EAA4; GLR6; GluK2; GLUK6; GLUR6; MRT6 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222369 representing NM_021956
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATAGTTTTGAAGCATCCAAGAAAGACCTTTATCTCCTCTTCAGCCTGTGATCAGCTGTCTCTTGGGG TGGCTGCCATCTTCGGGCCTTCACACAGCTCATCAGCAAACGCAGTGCAGTCCATCTGCAATGCTCTGGG AGTTCCCCACATACAGACCCGCTGGAAGCACCAGGTGTCAGACAACAAAGATTCCTTCTATGTCAGTCTC TACCCAGACTTCTCTTCACTCAGCCGTGCCATTTTAGACCTGGTGCAGTTTTTCAAGTGGAAAACCGTCA CGGTTGTGTATGATGACAGCACTGGTCTCATTCGTTTGCAAGAGCTCATCAAAGCTCCATCAAGGTATAA TCTTCGACTCAAAATTCGTCAGTTACCTGCTGATACAAAGGATGCAAAACCCTTACTAAAAGAAATGAAA AGAGGCAAGGAGTTTCATGTAATCTTTGATTGTAGCCATGAAATGGCAGCAGGCATTTTAAAACAGGCAT TAGCTATGGGAATGATGACAGAATACTATCATTATATCTTTACCACTCTGGACCTCTTTGCTCTTGATGT TGAGCCCTACCGATACAGTGGTGTTAACATGACAGGGTTCAGAATATTAAATACAGAAAATACCCAAGTC TCCTCCATCATTGAAAAGTGGTCGATGGAACGATTGCAGGCACCTCCGAAACCCGATTCAGGTTTGCTGG ATGGATTTATGACGACTGATGCTGCTCTAATGTATGATGCTGTGCATGTGGTGTCTGTGGCCGTTCAACA GTTTCCCCAGATGACAGTCAGTTCCTTGCAGTGTAATCGACATAAACCCTGGCGCTTCGGGACCCGCTTT ATGAGTCTAATTAAAGAGGCACATTGGGAAGGCCTCACAGGCAGAATAACTTTCAACAAAACCAATGGCT TGAGAACAGATTTTGATTTGGATGTGATCAGTCTGAAGGAAGAAGGTCTAGAAAAGATTGGAACGTGGGA TCCAGCCAGTGGCCTGAATATGACAGAAAGTCAAAAGGGAAAGCCAGCGAACATCACAGATTCGTTATCC AATCGTTCTTTGATTGTTACCACCATTTTGGAAGAGCCTTATGTCCTTTTTAAGAAGTCTGACAAACCTC TCTATGGTAATGATCGATTTGAAGGCTATTGCATTGATCTCCTCAGAGAGTTATCTACAATCCTTGGCTT TACATATGAAATTAGACTTGTGGAAGATGGGAAATATGGAGCCCAGGATGATGCCAATGGACAATGGAAT GGAATGGTTCGTGAACTAATTGATCATAAAGCTGACCTTGCAGTTGCTCCACTGGCTATTACCTATGTTC GAGAGAAGGTCATCGACTTTTCCAAGCCCTTTATGACACTTGGAATAAGTATTTTGTACCGCAAGCCCAA TGGTACAAACCCAGGCGTCTTCTCCTTCCTGAATCCTCTCTCCCCTGATATCTGGATGTATATTCTGCTG GCTTGCTTGGGTGTCAGTTGTGTGCTCTTTGTCATAGCCAGGTTTAGTCCTTATGAGTGGTATAATCCAC ACCCTTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACCTTGCTAAATAGTTTCTGGTTTGGAGT TGGAGCTCTCATGCGGCAAGGTTCTGAGCTCATGCCCAAAGCACTGTCCACCAGGATAGTGGGAGGCATT TGGTGGTTTTTCACACTTATCATCATTTCTTCGTATACTGCTAACTTAGCCGCCTTTCTGACAGTGGAAC GCATGGAATCCCCTATTGACTCTGCTGATGATTTAGCTAAACAAACCAAGATAGAATATGGAGCAGTAGA GGATGGTGCAACCATGACTTTTTTCAAGAAATCAAAAATCTCCACGTATGACAAAATGTGGGCCTTTATG AGTAGCAGAAGGCAGTCAGTGCTGGTCAAAAGTAATGAAGAAGGAATCCAGCGAGTCCTCACCTCTGATT ATGCTTTCCTAATGGAGTCAACAACCATCGAGTTTGTTACCCAGCGGAACTGTAACCTGACACAGATTGG CGGCCTTATAGACTCTAAAGGTTATGGCGTTGGCACTCCCATGGGTTCTCCATATCGAGACAAAATTACC ATAGCAATTCTTCAGCTGCAAGAGGAAGGCAAACTGCATATGATGAAGGAGAAATGGTGGAGGGGCAATG GTTGCCCAGAAGAGGAGAGCAAAGAGGCCAGTGCCCTGGGGGTTCAGAATATTGGTGGCATCTTCATTGT TCTGGCAGCCGGCTTGGTGCTTTCAGTTTTTGTGGCAGTGGGAGAATTTTTATACAAATCCAAAAAAAAC GCTCAATTGGAAAAGAGGTCCTTCTGTAGTGCCATGGTAGAAGAATTGAGGATGTCCCTGAAGTGCCAGC GTCGGTTAAAACATAAGCCACAGGCCCCAGTTATTGTGAAAACAGAAGAAGTTATCAACATGCACACATT TAACGACAGAAGGTTGCCAGGTAAAGAAACCATGGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222369 representing NM_021956
Red=Cloning site Green=Tags(s) MIVLKHPRKTFISSSACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSL YPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKDAKPLLKEMK RGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFRILNTENTQV SSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRHKPWRFGTRF MSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGKPANITDSLS NRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGAQDDANGQWN GMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYILL ACLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMRQGSELMPKALSTRIVGGI WWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKISTYDKMWAFM SSRRQSVLVKSNEEGIQRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPMGSPYRDKIT IAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVGEFLYKSKKN AQLEKRSFCSAMVEELRMSLKCQRRLKHKPQAPVIVKTEEVINMHTFNDRRLPGKETMA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021956 |
ORF Size | 2487 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021956.2, NP_068775.1 |
RefSeq Size | 3322 bp |
RefSeq ORF | 2727 bp |
Locus ID | 2898 |
UniProt ID | Q13002 |
Domains | lig_chan, ANF_receptor |
Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Elfn1-induced constitutive activation of mGluR7 determines frequency-dependent recruitment of SOM interneurons
,Jason Stachniak, T;Lauren Sylwestrak, E;Scheiffele, P;Hall, BJ;Ghosh, A;,
J. Neurosci.
,PubMed ID 30940718
[GRIK2]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC222369 | GRIK2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1 |
CNY 6,648.00 |
|
RC222369L1 | Lenti ORF clone of Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,048.00 |
|
RC222369L2 | Lenti ORF clone of Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
RC222369L3 | Lenti ORF clone of Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
RC222369L4 | Lenti ORF clone of Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
SC121951 | GRIK2 (untagged)-Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1 |
CNY 6,656.00 |