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UBF1 (UBTF) (NM_014233) Human Tagged ORF Clone
CAT＃: RG221399
- TrueORF®
UBTF (tGFP-tagged) - Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1
ORF Plasmid: DDK
"NM_014233" in other vectors (4)
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CNY 9,992.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CONDBA; NOR-90; UBF; UBF-1; UBF1; UBF2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG221399 representing NM_014233
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGGAGAAGCCGACTGCCCCACAGACCTGGAAATGGCCGCCCCCAAAGGCCAAGACCGTTGGTCCC AGGAAGACATGCTGACTTTGCTGGAATGCATGAAGAACAACCTTCCATCCAATGACAGCTCCAAGTTCAA AACCACCGAATCACACATGGACTGGGAAAAAGTAGCATTTAAAGACTTTTCTGGAGACATGTGCAAGCTC AAATGGGTGGAGATTTCTAATGAGGTGAGGAAGTTCCGTACATTGACAGAATTGATCCTCGATGCTCAGG AACATGTTAAAAATCCTTACAAAGGCAAAAAACTCAAGAAACACCCAGACTTCCCAAAGAAGCCCCTGAC CCCTTATTTCCGCTTCTTCATGGAGAAGCGGGCCAAGTATGCGAAACTCCACCCTGAGATGAGCAACCTG GACCTAACCAAGATTCTGTCCAAGAAATACAAGGAGCTTCCGGAGAAGAAGAAGATGAAATATATTCAGG ACTTCCAGAGAGAGAAACAGGAGTTCGAGCGAAACCTGGCCCGATTCAGGGAGGATCACCCCGACCTAAT CCAGAATGCCAAGAAATCGGACATCCCAGAGAAGCCCAAAACCCCCCAGCAGCTGTGGTACACCCACGAG AAGAAGGTGTATCTCAAAGTGCGGCCAGATGCCACTACGAAGGAGGTGAAGGACTCCCTGGGGAAGCAGT GGTCTCAGCTCTCGGACAAAAAGAGGCTGAAATGGATTCATAAGGCCCTGGAGCAGCGGAAGGAGTACGA GGAGATCATGAGAGACTATATCCAGAAGCACCCAGAGCTGAACATCAGTGAGGAGGGTATCACCAAGTCC ACCCTCACCAAGGCCGAACGCCAGCTCAAGGACAAGTTTGACGGGCGACCCACCAAGCCACCTCCGAACA GCTACTCGCTGTACTGCGCAGAGCTCATGGCCAACATGAAGGACGTGCCCAGCACAGAGCGCATGGTGCT GTGCAGCCAGCAGTGGAAGCTGCTGTCCCAGAAGGAGAAGGACGCCTATCACAAGAAGTGTGATCAGAAA AAGAAAGATTACGAGGTGGAGCTGCTCCGTTTCCTCGAGAGCCTGCCTGAGGAGGAGCAGCAGCGGGTCT TGGGGGAAGAGAAGATGCTGAACATCAACAAGAAGCAGGCCACCAGCCCCGCCTCCAAGAAGCCAGCCCA GGAAGGGGGCAAGGGCGGCTCCGAGAAGCCCAAGCGGCCCGTGTCGGCCATGTTCATCTTCTCGGAGGAG AAACGGCGGCAGCTGCAGGAGGAGCGGCCTGAGCTCTCCGAGAGCGAGCTGACCCGCCTGCTGGCCCGAA TGTGGAACGACCTGTCTGAGAAGAAGAAGGCCAAGTACAAGGCCCGAGAGGCGGCGCTCAAGGCTCAGTC GGAGAGGAAGCCCGGCGGGGAGCGCGAGGAACGGGGCAAGCTGCCCGAGTCCCCCAAAAGAGCTGAGGAG ATCTGGCAACAGAGCGTTATCGGCGACTACCTGGCCCGCTTCAAGAATGACCGGGTGAAGGCCTTGAAAG CCATGGAAATGACCTGGAATAACATGGAAAAGAAGGAGAAACTGATGTGGATTAAGAAAGCAGCCGAAGA CCAAAAGCGATATGAGAGAGAGCTGAGTGAGATGCGGGCACCTCCAGCTGCTACAAATTCTTCCAAGAAG ATGAAATTCCAGGGAGAACCCAAGAAGCCTCCCATGAACGGTTACCAGAAGTTCTCCCAGGAGCTGCTGT CCAATGGGGAGCTGAACCACCTGCCGCTGAAGGAGCGCATGGTGGAGATCGGCAGTCGCTGGCAGCGCAT CTCCCAGAGCCAGAAGGAGCACTACAAAAAGCTGGCCGAGGAGCAGCAAAAGCAGTACAAGGTGCACCTG GACCTCTGGGTTAAGAGCCTGTCTCCCCAGGACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTA AGAGCATGACCAAGCTGCGAGGCCCAAACCCCAAATCCAGCCGGACTACTCTGCAGTCCAAGTCGGAGTC CGAGGAGGATGATGAAGAGGATGAGGATGACGAGGACGAGGATGAAGAAGAGGAAGATGATGAGAATGGG GACTCCTCTGAAGATGGCGGCGACTCCTCTGAGTCCAGCAGCGAGGACGAGAGCGAGGATGGGGATGAGA ATGAAGAGGATGACGAGGACGAAGACGACGACGAGGATGACGATGAGGATGAAGATAATGAGTCCGAGGG CAGCAGCTCCAGCTCCTCCTCCTCAGGGGACTCCTCAGACTCTGACTCCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221399 representing NM_014233
Red=Cloning site Green=Tags(s) MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKL KWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNL DLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHE KKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKS TLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQK KKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEE KRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEE IWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKK MKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHL DLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENG DSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014233 |
| ORF Size | 2292 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014233.1, NP_055048.1 |
| RefSeq Size | 3097 bp |
| RefSeq ORF | 2295 bp |
| Locus ID | 7343 |
| UniProt ID | P17480 |
| Domains | HMG |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221399 | UBTF (Myc-DDK-tagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 5,800.00 |
|
| RC221399L3 | Lenti-ORF clone of UBTF (Myc-DDK-tagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 7,700.00 |
|
| RC221399L4 | Lenti-ORF clone of UBTF (mGFP-tagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 7,700.00 |
|
| SC115105 | UBTF (untagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 8,400.00 |
