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UBF1 (UBTF) (NM_014233) Human Untagged Clone
CAT＃: SC115105
UBTF (untagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1
CNY 8,400.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CONDBA; NOR-90; UBF; UBF-1; UBF1; UBF2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_014233 edited
ATGAACGGAGAAGCCGACTGCCCCACAGACCTGGAAATGGCCGCCCCCAAAGGCCAAGAC CGTTGGTCCCAGGAAGACATGCTGACTTTGCTGGAATGCATGAAGAACAACCTTCCATCC AATGACAGCTCCAAGTTCAAAACCACCGAATCACACATGGACTGGGAAAAAGTAGCATTT AAAGACTTTTCTGGAGACATGTGCAAGCTCAAATGGGTGGAGATTTCTAATGAGGTGAGG AAGTTCCGTACATTGACAGAATTGATCCTCGATGCTCAGGAACATGTTAAAAATCCTTAC AAAGGCAAAAAACTCAAGAAACACCCAGACTTCCCAAAGAAGCCCCTGACCCCTTATTTC CGCTTCTTCATGGAGAAGCGGGCCAAGTATGCGAAACTCCACCCTGAGATGAGCAACCTG GACCTAACCAAGATTCTGTCCAAGAAATACAAGGAGCTTCCGGAGAAGAAGAAGATGAAA TATATTCAGGACTTCCAGAGAGAGAAACAGGAGTTCGAGCGAAACCTGGCCCGATTCAGG GAGGATCACCCCGACCTAATCCAGAATGCCAAGAAATCGGACATCCCAGAGAAGCCCAAA ACCCCCCAGCAGCTGTGGTACACCCACGAGAAGAAGGTGTATCTCAAAGTGCGGCCAGAT GCCACTACGAAGGAGGTGAAGGACTCCCTGGGGAAGCAGTGGTCTCAGCTCTCGGACAAA AAGAGGCTGAAATGGATTCATAAGGCCCTGGAGCAGCGGAAGGAGTACGAGGAGATCATG AGAGACTATATCCAGAAGCACCCAGAGCTGAACATCAGTGAGGAGGGTATCACCAAGTCC ACCCTCACCAAGGCCGAACGCCAGCTCAAGGACAAGTTTGACGGGCGACCCACCAAGCCA CCTCCGAACAGCTACTCGCTGTACTGCGCAGAGCTCATGGCCAACATGAAGGACGTGCCC AGCACAGAGCGCATGGTGCTGTGCAGCCAGCAGTGGAAGCTGCTGTCCCAGAAGGAGAAG GACGCCTATCACAAGAAGTGTGATCAGAAAAAGAAAGATTACGAGGTGGAGCTGCTCCGT TTCCTCGAGAGCCTGCCTGAGGAGGAGCAGCAGCGGGTCTTGGGGGAAGAGAAGATGCTG AACATCAACAAGAAGCAGGCCACCAGCCCCGCCTCCAAGAAGCCAGCCCAGGAAGGGGGC AAGGGCGGCTCCGAGAAGCCCAAGCGGCCCGTGTCGGCCATGTTCATCTTCTCGGAGGAG AAACGGCGGCAGCTGCAGGAGGAGCGGCCTGAGCTCTCCGAGAGCGAGCTGACCCGCCTG CTGGCCCGAATGTGGAACGACCTGTCTGAGAAGAAGAAGGCCAAGTACAAGGCCCGAGAG GCGGCGCTCAAGGCTCAGTCGGAGAGGAAGCCCGGCGGGGAGCGCGAGGAACGGGGCAAG CTGCCCGAGTCCCCCAAAAGAGCTGAGGAGATCTGGCAACAGAGCGTTATCGGCGACTAC CTGGCCCGCTTCAAGAATGACCGGGTGAAGGCCTTGAAAGCCATGGAAATGACCTGGAAT AACATGGAAAAGAAGGAGAAACTGATGTGGATTAAGAAAGCAGCCGAAGACCAAAAGCGA TATGAGAGAGAGCTGAGTGAGATGCGGGCACCTCCAGCTGCTACAAATTCTTCCAAGAAG ATGAAATTCCAGGGAGAACCCAAGAAGCCTCCCATGAACGGTTACCAGAAGTTCTCCCAG GAGCTGCTGTCCAATGGGGAGCTGAACCACCTGCCGCTGAAGGAGCGCATGGTGGAGATC GGCAGTCGCTGGCAGCGCATCTCCCAGAGCCAGAAGGAGCACTACAAAAAGCTGGCCGAG GAGCAGCAAAAGCAGTACAAGGTGCACCTGGACCTCTGGGTTAAGAGCCTGTCTCCCCAG GACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTAAGAGCATGACCAAGCTGCGA GGCCCAAACCCCAAATCCAGCCGGACTACTCTGCAGTCCAAGTCGGAGTCCGAGGAGGAT GATGAAGAGGATGAGGATGACGAGGACGAGGATGAAGAAGAGGAAGATGATGAGAATGGG GACTCCTCTGAAGATGGCGGCGACTCCTCTGAGTCCAGCAGCGAGGACGAGAGCGAGGAT GGGGATGAGAATGAAGAGGATGACGAGGACGAAGACGACGACGAGGATGACGATGAGGAT GAAGATAATGAGTCCGAGGGCAGCAGCTCCAGCTCCTCCTCCTCAGGGGACTCCTCAGAC TCTGACTCCAACTGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014233 |
| Insert Size | 2920 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014233.1, NP_055048.1 |
| RefSeq Size | 3097 bp |
| RefSeq ORF | 2295 bp |
| Locus ID | 7343 |
| UniProt ID | P17480 |
| Domains | HMG |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of DHX33 as a Mediator of rRNA Synthesis and Cell Growth
,Yandong Zhang, Jason T. Forys, Alexander P. Miceli, Abigail S. Gwinn, and Jason D. Weber,
Mol. Cell. Biol., Dec 2011; 31: 4676 - 4691.
[UBTF]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221399 | UBTF (Myc-DDK-tagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 5,800.00 |
|
| RC221399L3 | Lenti-ORF clone of UBTF (Myc-DDK-tagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 7,700.00 |
|
| RC221399L4 | Lenti-ORF clone of UBTF (mGFP-tagged)-Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 7,700.00 |
|
| RG221399 | UBTF (tGFP-tagged) - Human upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 1 |
CNY 9,992.00 |
