Achaete scute homolog 3 (ASCL3) (NM_020646) Human Tagged ORF Clone

CAT#: RG220042

  • TrueORF®

ASCL3 (tGFP-tagged) - Human achaete-scute complex homolog 3 (Drosophila) (ASCL3)

ORF Plasmid: DDK tGFP



  "NM_020646" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
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Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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ASCL3 mouse monoclonal antibody,clone OTI1C5
    • 100 ul

CNY 1,999.00
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms bHLHa42; HASH3; SGN1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG220042 representing NM_020646
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGACAACAGAGGCAACTCTAGTCTACCTGACAAACTTCCTATCTTCCCTGATTCTGCCCGCTTGC
CACTGACCAGGTCCTTCTATCTGGAGCCCATGGTCACTTTCCACGTGCACCCAGAGGCCCCGGTGTCATC
CCCTTACTCTGAGGAGCTGCCACGGCTGCCTTTTCCCAGCGACTCTCTTATCCTGGGAAATTACAGTGAA
CCCTGCCCCTTCTCTTTCCCGATGCCTTATCCAAATTACAGAGGGTGCGAGTACTCCTACGGGCCAGCCT
TCACCCGGAAAAGGAATGAGCGGGAAAGGCAGCGGGTGAAATGTGTCAATGAAGGCTATGCCCAGCTCCG
CCATCATCTGCCAGAGGAGTATTTGGAGAAGCGACTCAGCAAAGTGGAAACCCTCAGAGCTGCGATCAAG
TACATTAACTACCTGCAGTCTCTTCTGTACCCTGATAAAGCTGAGACCAAGAATAACCCTGGAAAAGTTT
CCTCCATGATAGCAACCACCAGCCACCATGCTGACCCTATGTTCAGAATTGTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG220042 representing NM_020646
Red=Cloning site Green=Tags(s)

MMDNRGNSSLPDKLPIFPDSARLPLTRSFYLEPMVTFHVHPEAPVSSPYSEELPRLPFPSDSLILGNYSE
PCPFSFPMPYPNYRGCEYSYGPAFTRKRNERERQRVKCVNEGYAQLRHHLPEEYLEKRLSKVETLRAAIK
YINYLQSLLYPDKAETKNNPGKVSSMIATTSHHADPMFRIV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020646
ORF Size 543 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020646.1, NP_065697.1
RefSeq Size 650 bp
RefSeq ORF 546 bp
Locus ID 56676
UniProt ID Q9NQ33
Gene Summary Basic helix-loop-helix transcription factors, such as ASCL3, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).[supplied by OMIM, Mar 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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