ZNF451 (NM_001031623) Human Tagged ORF Clone
CAT#: RG215720
- TrueORF®
ZNF451 (tGFP-tagged) - Human zinc finger protein 451 (ZNF451), transcript variant 1
ORF Plasmid: DDK
"NM_001031623" in other vectors (6)
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CNY 12,350.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | COASTER; dJ417I1.1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215720 representing NM_001031623
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACCCGGGGTCGGAAATAATAGAATCTGTCCCTCCAGCTGGCCCTGAGGCATCTGAGTCAACAA CGGATGAAAATGAAGACGACATTCAGTTTGTCAGTGAAGGACCATTACGACCTGTTCTTGAATACATTGA TCTGGTCAGCAGTGATGATGAAGAGCCTAGCACCTCTTATACTGATGAGAATATTAAACGTAAAGACCAT ATTGATTATCAGAAGGATAAAGTTGCTTTAACTCTGGCTCGTCTAGCCCGCCATGTTGAAGTGGAGAAAC AGCAGAAAGAAGAGAAGAATAGAGCATTCAGAGAAAAAATTGATTTTCAGCATGCTCATGGGTTACAAGA ATTGGAATTTATTCGAGGACATTCTGATACAGAAGCAGCAAGACTGTGTGTGGACCAGTGGCTAAAAATG CCAGGACTCAAAACAGGCACAATTAATTGTGGAACAAAAAGTTCATTCCGAAGAGGAGGCCACACGTGGG TGTCTGGGAAACCAATTTTATGTCCTATAATGCACTGTAACAAGGAGTTTGACAATGGGCACCTTCTCTT AGGACATTTGAAAAGGTTCGATCACTCTCCATGTGATCCAACAATTACACTACATGGACCTTTCTTCAGC TCCTTTGCTTGTGTAGTATGTTATAAAAAATTTGTTACTCAACAACAATATAGAGATCACCTTTTTGATA AGGAAGCCACAGATGATGGACATAACAACAACCTTCTTCCTCAGATTATTCAGTGTTTTGCATGTCCAAA TTGCTTCCTTCTTTTTAGCAGAAAGGAGGAGTGTTCAAAGCATATGTCTGGAAAGAATCATTTCCATCAG AGTTTCAAACTGGGTGATAACAAAGGAATTGCACATCCAATATCTTTCCCATCTTTTGCAAAGAAACTTT TGATCTCTCTGTGCAAAGATGTTCCCTTTCAAGTTAAGTGTGTGGCCTGCCACAAGACACTGCGTTCCCA CATGGAGCTCACTGCCCATTTCAGAGTTCATTGTCGAAATGCTGGACCTGTAGCTGTAGCTGAGAAGAGC ATTACCCAGGTTGCAGAGAAATTCATATTAAGAGGTTATTGTCCAGATTGCAATCAAGTCTTTGTGGATG AAACCAGCACCCAAAATCATAAGCAGAATTCAGGACACAAAGTCCGAGTCATTAACTCAGTGGAAGAATC AGTCTTACTCTATTGCCACAGCAGCGAAGGGAACAAGGATCCTTCTTCTGACTTGCATTTATTGTTGGAT CAATCAAAATTTTCATCACTTAAAAGAACCATGTCTATTAAAGAATCTAGCTCACTGGAGTGCATTGCCA TTCCAAAAAAGAAGATGAATTTAAAAGATAAAAGCCATGAAGGTGTTGCTTGTGTCCAGAAAGAAAAATC AGTAGTTAAAACCTGGTTCTGTGAATGCAATCAGCGATTCCCAAGTGAAGATGCAGTAGAAAAGCATGTT TTCTCAGCAAACACAATGGGTTATAAATGTGTGGTCTGTGGAAAGGTATGTGATGATTCAGGGGTCATTC GTTTACACATGAGCCGGATTCACGGAGGGGCACATTTAAATAACTTTCTTTTCTGGTGTCGGACATGCAA AAAGGAGTTAACAAGGAAAGATACTATCATGGCACATGTGACTGAATTTCATAATGGACACAGATATTTT TATGAGATGGATGAGGTAGAAGGTGAAACTTTGCCATCATCCTCTACAACATTGGATAATTTGACTGCTA ACAAGCCTTCATCAGCTATTACTGTTATTGATCATTCCCCGGCAAATAGTTCTCCGAGGGGTAAATGGCA ATGCCGGATTTGTGAAGATATGTTTGATTCCCAGGAATATGTAAAACAGCACTGCATGTCTTTGGCAAGC CACAAGTTTCATAGATACAGCTGTGCTCACTGCAGAAAGCCTTTTCATAAGATAGAAACATTGTACCGAC ATTGCCAAGATGAGCATGACAATGAGATAAAGATTAAATACTTCTGTGGGCTTTGTGATCTTATCTTTAA TGTGGAAGAAGCATTTCTGAGTCATTATGAGGAGCACCACAGCATAGATTATGTATTTGTGTCAGAAAAA ACTGAAACTTCAATTAAAACCGAAGATGATTTTCCAGTAATAGAGACCAGTAACCAGTTAACTTGTGGTT GCCGTGAGAGTTACATCTGTAAAGTCAACAGAAAAGAAGATTATAGCAGATGTCTCCAAATCATGCTGGA TAAAGGAAAACTGTGGTTTCGCTGCAGTTTATGTTCGGCAACAGCACAGAATTTAACCGACATGAACACT CATATCCATCAAGTGCACAAAGAAAAGAGTGATGAGGAGGAGCAGCAGTATGTAATCAAGTGTGGCACCT GCACCAAAGCATTTCATGATCCTGAGAGTGCACAGCAGCATTTCCATAGAAAACATTGCTTCTTACAGAA ACCCAGTGTGGCTCATTTTGGATCTGAAAAATCAAACCTGTACAAGTTTACTGCTAGTGCCTCACATACA GAGAGAAAACTGAAACAGGCAATAAACTATTCAAAAAGTTTAGACATGGAGAAAGGAGTTGAGAATGACC TAAGCTATCAGAATATAGAGGAAGAAATTGTTGAGCTTCCAGATTTGGATTACCTGCGAACCATGACTCA TATAGTCTTTGTAGATTTTGATAACTGGTCAAACTTTTTTGGTCATCTACCAGGGCATCTAAACCAAGGA ACATTTATTTGGGGCTTTCAAGGAGGAAACACCAATTGGAAGCCTCCGCTCAACTGTAAGATTTATAACT ACCTGAACAGGATTGGATGCTTCTTCCTTCATCCTCGCTGTAGTAAAAGAAAAGATGCTGCTGATTTTGC CATATGTATGCATGCTGGCCGTCTAGATGAACAACTACCCAAGCAAATTCCTTTCACCATCCTCTCAGGA GATCAAGGTTTTCTGGAGCTAGAGAATCAATTTAAGAAGACTCAGAGGCCAGCTCATATACTAAACCCTC ACCACTTAGAGGGAGATATGATGTGTGCCTTGTTAAATAGCATATCTGATACCACCAAAGAATGTGACAG TGATGATAACATGGGTGCCAAAAATACTTCAATAGGAGAAGAATTTATATCCACAGAAGATGTGGAATTA GAAGAAGCTATTAGAAGAAGTCTTGAGGAAATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215720 representing NM_001031623
Red=Cloning site Green=Tags(s) MGDPGSEIIESVPPAGPEASESTTDENEDDIQFVSEGPLRPVLEYIDLVSSDDEEPSTSYTDENIKRKDH IDYQKDKVALTLARLARHVEVEKQQKEEKNRAFREKIDFQHAHGLQELEFIRGHSDTEAARLCVDQWLKM PGLKTGTINCGTKSSFRRGGHTWVSGKPILCPIMHCNKEFDNGHLLLGHLKRFDHSPCDPTITLHGPFFS SFACVVCYKKFVTQQQYRDHLFDKEATDDGHNNNLLPQIIQCFACPNCFLLFSRKEECSKHMSGKNHFHQ SFKLGDNKGIAHPISFPSFAKKLLISLCKDVPFQVKCVACHKTLRSHMELTAHFRVHCRNAGPVAVAEKS ITQVAEKFILRGYCPDCNQVFVDETSTQNHKQNSGHKVRVINSVEESVLLYCHSSEGNKDPSSDLHLLLD QSKFSSLKRTMSIKESSSLECIAIPKKKMNLKDKSHEGVACVQKEKSVVKTWFCECNQRFPSEDAVEKHV FSANTMGYKCVVCGKVCDDSGVIRLHMSRIHGGAHLNNFLFWCRTCKKELTRKDTIMAHVTEFHNGHRYF YEMDEVEGETLPSSSTTLDNLTANKPSSAITVIDHSPANSSPRGKWQCRICEDMFDSQEYVKQHCMSLAS HKFHRYSCAHCRKPFHKIETLYRHCQDEHDNEIKIKYFCGLCDLIFNVEEAFLSHYEEHHSIDYVFVSEK TETSIKTEDDFPVIETSNQLTCGCRESYICKVNRKEDYSRCLQIMLDKGKLWFRCSLCSATAQNLTDMNT HIHQVHKEKSDEEEQQYVIKCGTCTKAFHDPESAQQHFHRKHCFLQKPSVAHFGSEKSNLYKFTASASHT ERKLKQAINYSKSLDMEKGVENDLSYQNIEEEIVELPDLDYLRTMTHIVFVDFDNWSNFFGHLPGHLNQG TFIWGFQGGNTNWKPPLNCKIYNYLNRIGCFFLHPRCSKRKDAADFAICMHAGRLDEQLPKQIPFTILSG DQGFLELENQFKKTQRPAHILNPHHLEGDMMCALLNSISDTTKECDSDDNMGAKNTSIGEEFISTEDVEL EEAIRRSLEEM TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001031623 |
ORF Size | 3183 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001031623.1, NP_001026794.1 |
RefSeq Size | 5060 bp |
RefSeq ORF | 3186 bp |
Locus ID | 26036 |
UniProt ID | Q9Y4E5 |
Protein Families | Transcription Factors |
Gene Summary | E3 SUMO-protein ligase; has a preference for SUMO2 and SUMO3 and facilitates UBE2I/UBC9-mediated sumoylation of target proteins (PubMed:26524493, PubMed:26524494). Plays a role in protein SUMO2 modification in response to stress caused by DNA damage and by proteasome inhibitors (in vitro). Required for MCM4 sumoylation (By similarity). Has no activity with SUMO1 (PubMed:26524493). Preferentially transfers an additional SUMO2 chain onto the SUMO2 consensus site 'Lys-11' (PubMed:26524493). Negatively regulates transcriptional activation mediated by the SMAD4 complex in response to TGF-beta signaling. Inhibits EP300-mediated acetylation of histone H3 at 'Lys-9' (PubMed:24324267). Plays a role in regulating the transcription of AR targets (PubMed:18656483).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215720 | ZNF451 (Myc-DDK-tagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1 |
CNY 7,392.00 |
|
RC215720L1 | Lenti-ORF clone of ZNF451 (Myc-DDK-tagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1 |
CNY 9,792.00 |
|
RC215720L2 | Lenti-ORF clone of ZNF451 (mGFP-tagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1 |
CNY 13,110.00 |
|
RC215720L3 | Lenti-ORF clone of ZNF451 (Myc-DDK-tagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1 |
CNY 13,110.00 |
|
RC215720L4 | Lenti-ORF clone of ZNF451 (mGFP-tagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1 |
CNY 13,110.00 |
|
SC302520 | ZNF451 (untagged)-Human zinc finger protein 451 (ZNF451), transcript variant 1 |
CNY 20,430.00 |