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C1orf69 (IBA57) (NM_001010867) Human Tagged ORF Clone
CAT＃: RG211947
- TrueORF®
IBA57 (tGFP-tagged) - Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57)
ORF Plasmid: DDK
"NM_001010867" in other vectors (6)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C1orf69; MMDS3; SPG74 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211947 representing NM_001010867
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGCGGCGCTGCTTCGAGGCGCCACTCCGGGGCGCGGCGGCCCGGTCTGGCGCTGGCGGCTGC GCGCGGCCCCAAGGTGCCGCCTGGCCCACAGCTCCTGCAGTCCTGGTGGCGACCCAACGGCCGGAGCGGC CTGGGCCTGCTTCCGGCTGGACGGGCGCACCCTGCTGCGCGTGCGTGGCCCCGACGCGGCGCCCTTCCTG CTAGGGCTGCTGACCAATGAACTGCCGCTTCCGAGTCCTGCGGCCGCGGGGGCCCCGCCTGCTGCGCGCG CGGGCTACGCCCACTTCCTGAACGTGCAGGGCCGGACGCTCTATGACGTCATCTTGTACGGGCTCCAGGA ACACTCGGAGGTGTCTGGCTTCCTTCTGGAGTGTGACAGCTCGGTGCAGGGCGCGCTGCAGAAGCACCTC GCGCTATACAGGATCCGGCGGAAGGTCACGGTGGAGCCGCACCCGGAGCTGCGAGTGTGGGCGGTGTTGC CCAGTTCCCCTGAGGCCTGCGGGGCTGCATCGCTGCAGGAGAGGGCAGGGGCTGCCGCCATCCTCATCCG CGACCCGCGAACAGCACGCATGGGGTGGCGGCTCCTCACCCAGGATGAAGGCCCAGCCCTGGTGCCCGGG GGCCGGCTCGGGGACTTGTGGGATTATCACCAGCACCGATACCTGCAAGGCGTTCCTGAGGGGGTCCGAG ACTTGCCTCCTGGGGTGGCCCTGCCCCTGGAGTCCAACCTGGCCTTCATGAACGGCGTGAGCTTCACCAA AGGCTGCTACATTGGCCAGGAGCTGACGGCCCGCACCCACCACATGGGCGTCATCCGCAAGCGCCTCTTC CCTGTCCGGTTCTTGGACCCCCTTCCCACCAGTGGCATCACCCCTGGTGCCACGGTGCTGACTGCCTCAG GACAGACTGTGGGCAAGTTCAGGGCTGGCCAGGGCAACGTGGGGCTGGCCCTGCTGTGGTCAGAGAAGAT CAAGGGTCCTCTGCACATCAGAGCCTCTGAGGGTGCCCAGGTGGCCTTAGCCGCATCTGTGCCAGACTGG TGGCCTACAGTCTCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211947 representing NM_001010867
Red=Cloning site Green=Tags(s) MATAALLRGATPGRGGPVWRWRLRAAPRCRLAHSSCSPGGDPTAGAAWACFRLDGRTLLRVRGPDAAPFL LGLLTNELPLPSPAAAGAPPAARAGYAHFLNVQGRTLYDVILYGLQEHSEVSGFLLECDSSVQGALQKHL ALYRIRRKVTVEPHPELRVWAVLPSSPEACGAASLQERAGAAAILIRDPRTARMGWRLLTQDEGPALVPG GRLGDLWDYHQHRYLQGVPEGVRDLPPGVALPLESNLAFMNGVSFTKGCYIGQELTARTHHMGVIRKRLF PVRFLDPLPTSGITPGATVLTASGQTVGKFRAGQGNVGLALLWSEKIKGPLHIRASEGAQVALAASVPDW WPTVSK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001010867 |
| ORF Size | 1068 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001010867.4 |
| RefSeq Size | 7817 bp |
| RefSeq ORF | 1071 bp |
| Locus ID | 200205 |
| UniProt ID | Q5T440 |
| Gene Summary | The protein encoded by this gene localizes to the mitochondrion and is part of the iron-sulfur cluster assembly pathway. The encoded protein functions late in the biosynthesis of mitochondrial 4Fe-4S proteins. Defects in this gene have been associated with autosomal recessive spastic paraplegia-74 and with multiple mitochondrial dysfunctions syndrome-3. Two transcript variants encoding different isoforms have been found for this gene. The smaller isoform is not likely to be localized to the mitochondrion since it lacks the amino-terminal transit peptide. [provided by RefSeq, Jul 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211947 | IBA57 (Myc-DDK-tagged)-Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57) |
CNY 3,656.00 |
|
| RC211947L1 | Lenti ORF clone of Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211947L2 | Lenti ORF clone of Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57), mGFP tagged |
CNY 5,890.00 |
|
| RC211947L3 | Lenti ORF clone of Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211947L4 | Lenti ORF clone of Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57), mGFP tagged |
CNY 5,890.00 |
|
| SC301491 | IBA57 (untagged)-Human IBA57, iron-sulfur cluster assembly homolog (S. cerevisiae) (IBA57) |
CNY 5,800.00 |
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