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Endostatin (COL18A1) (NM_130445) Human Tagged ORF Clone
CAT＃: RG209465
- TrueORF®
COL18A1 (tGFP-tagged) - Human collagen, type XVIII, alpha 1 (COL18A1), transcript variant 2
ORF Plasmid: DDK
"NM_130445" in other vectors (2)
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CNY 14,630.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | KNO; KNO1; KS |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG209465 representing NM_130445
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGAGGTGCCCCTGGCCATGGCCGCGGCGGCGGCGCCTCCTGGACGTGCTCGCGCCCCTGGTCC TGCTGCTCGGGGTCCGCGCGGCCTCCGCGGAGCCAGAGCGCATCAGCGAGGAGGTGGGGCTGCTGCAGCT CCTTGGGGACCCCCCGCCCCAGCAGGTCACCCAGACGGATGACCCCGACGTCGGGCTGGCCTACGTCTTT GGGCCAGATGCCAACAGTGGCCAAGTGGCCCGGTACCACTTCCCCAGCCTCTTCTTCCGTGACTTCTCAC TGCTGTTCCACATCCGGCCAGCCACAGAGGGCCCAGGGGTGCTGTTCGCCATCACGGACTCGGCGCAGGC CATGGTCTTGCTGGGCGTGAAGCTCTCTGGGGTGCAGGACGGGCACCAGGACATCTCCCTGCTCTACACA GAACCAGGTGCAGGCCAGACCCACACAGCCGCCAGCTTCCGGCTCCCCGCCTTCGTCGGCCAGTGGACAC ACTTAGCCCTCAGTGTGGCAGGTGGCTTTGTGGCCCTCTACGTGGACTGTGAGGAGTTCCAGAGAATGCC GCTTGCTCGGTCCTCACGGGGCCTGGAGCTGGAGCCTGGCGCCGGGCTCTTCGTGGCTCAGGCGGGGGGA GCGGACCCTGACAAGTTCCAGGGGGTGATCGCTGAGCTGAAGGTGCGCAGGGACCCCCAGGTGAGCCCCA TGCACTGCCTGGACGAGGAAGGCGATGACTCAGATGGGGCATCCGGAGACTCTGGCAGCGGGCTCGGGGA CGCCCGGGAGCTTCTCAGGGAGGAGACGGGCGCGGCCCTAAAACCCAGGCTCCCCGCGCCACCCCCCGTC ACCACGCCACCCTTGGCTGGAGGCAGCAGCACGGAAGATTCCAGAAGTGAAGAAGTCGAGGAGCAGACCA CGGTGGCTTCGTTAGGAGCTCAGACACTTCCTGGCTCAGATTCTGTCTCCACGTGGGACGGGAGTGTCCG GACCCCTGGGGGCCGCGTGAAAGAGGGCGGCCTGAAGGGGCAGAAAGGGGAGCCAGGTGTTCCGGGCCCA CCTGGCCGGGCAGGCCCCCCAGGATCCCCATGCCTACCTGGTCCCCCGGGTCTCCCGTGCCCAGTGAGTC CCCTGGGTCCTGCAGGCCCAGCGTTGCAAACTGTCCCCGGACCACAAGGACCCCCAGGGCCTCCGGGGAG GGACGGCACCCCTGGAAGGGACGGCGAGCCGGGCGACCCCGGTGAAGACGGAAAGCCGGGCGACACCGGG CCACAAGGCTTCCCCGGGACTCCAGGGGACGTAGGTCCCAAGGGCGACAAGGGAGACCCTGGGGTTGGAG AGAGAGGGCCCCCAGGACCCCAAGGGCCTCCAGGGCCCCCAGGACCCTCCTTCAGACACGACAAGCTGAC CTTCATTGACATGGAGGGATCTGGCTTCGGGGGCGATCTGGAGGCCCTGCGGGGTCCTCGAGGCTTCCCT GGACCTCCCGGACCCCCCGGTGTCCCAGGCCTGCCCGGCGAGCCAGGCCGCTTTGGGGTGAACAGCTCCG ACGTCCCAGGACCCGCCGGCCTTCCTGGTGTGCCTGGGCGCGAGGGTCCCCCCGGGTTTCCTGGCCTCCC GGGACCCCCAGGCCCTCCGGGAAGAGAGGGGCCCCCAGGAAGGACTGGGCAGAAAGGCAGCCTGGGTGAA GCAGGCGCCCCAGGACATAAGGGGAGCAAGGGAGCCCCCGGTCCTGCTGGTGCTCGTGGGGAGAGCGGCC TGGCAGGAGCCCCCGGACCTGCTGGACCACCAGGCCCCCCTGGGCCCCCTGGGCCCCCAGGACCAGGACT CCCCGCTGGATTTGATGACATGGAAGGCTCCGGGGGGCCCTTCTGGTCAACAGCCCGAAGCGCTGATGGG CCACAGGGACCTCCCGGCCTGCCGGGACTTAAGGGGGATCCTGGCGTGCCTGGGCTGCCGGGGGCGAAGG GAGAAGTTGGAGCAGATGGAGTCCCCGGGTTCCCCGGCCTCCCTGGCAGAGAGGGCATTGCTGGGCCCCA GGGGCCAAAGGGAGACAGAGGCAGCCGGGGAGAAAAGGGAGATCCAGGGAAGGACGGAGTCGGGCAGCCG GGCCTCCCTGGCCCCCCCGGACCCCCGGGACCTGTGGTCTACGTGTCGGAGCAGGACGGATCCGTCCTGA GCGTGCCGGGACCTGAGGGCCGGCCGGGTTTCGCAGGCTTTCCCGGACCTGCAGGACCCAAGGGCAACCT GGGCTCTAAGGGCGAACGAGGCTCCCCGGGACCCAAGGGTGAGAAGGGTGAACCGGGCAGCATCTTCAGC CCCGACGGCGGTGCCCTGGGCCCTGCCCAGAAAGGAGCCAAGGGAGAGCCGGGCTTCCGAGGACCCCCGG GTCCATACGGACGGCCGGGGTACAAGGGAGAGATTGGCTTTCCTGGACGGCCGGGTCGCCCCGGGATGAA CGGATTGAAAGGAGAGAAAGGGGAGCCGGGAGATGCCAGCCTTGGATTTGGCATGAGGGGAATGCCCGGC CCCCCAGGACCTCCAGGGCCCCCAGGCCCTCCAGGGACTCCTGTTTACGACAGCAATGTGTTTGCTGAGT CCAGCCGCCCCGGGCCTCCAGGATTGCCAGGGAATCAGGGCCCTCCAGGACCCAAGGGCGCCAAAGGAGA AGTGGGCCCCCCCGGACCACCAGGGCAGTTTCCGTTTGACTTTCTTCAGTTGGAGGCTGAAATGAAGGGG GAGAAGGGAGACCGAGGTGATGCAGGACAGAAAGGCGAAAGGGGGGAGCCCGGGGGCGGCGGTTTCTTCG GCTCCAGCCTGCCCGGCCCCCCCGGCCCCCCAGGCCCACGTGGCTACCCTGGGATTCCAGGTCCCAAGGG AGAGAGCATCCGGGGCCAGCCCGGCCCACCTGGACCTCAGGGACCCCCCGGCATCGGCTACGAGGGGCGC CAGGGCCCTCCCGGCCCCCCAGGCCCCCCAGGGCCCCCTTCATTTCCTGGCCCTCACAGGCAGACTATCA GCGTTCCCGGCCCTCCGGGCCCCCCTGGGCCCCCTGGGCCCCCTGGAACCATGGGCGCCTCCTCAGGGGT GAGGCTCTGGGCTACACGCCAGGCCATGCTGGGCCAGGTGCACGAGGTTCCCGAGGGCTGGCTCATCTTC GTGGCCGAGCAGGAGGAGCTCTACGTCCGCGTGCAGAACGGGTTCCGGAAGGTCCAGCTGGAGGCCCGGA CACCACTCCCACGAGGGACGGACAATGAAGTGGCCGCCTTGCAGCCCCCCGTGGTGCAGCTGCACGACAG CAACCCCTACCCGCGGCGGGAGCACCCCCACCCCACCGCGCGGCCCTGGCGGGCAGATGACATCCTGGCC AGCCCCCCTCGCCTGCCCGAGCCCCAGCCCTACCCCGGAGCCCCGCACCACAGCTCCTACGTGCACCTGC GGCCGGCACGACCCACAAGCCCACCCGCCCACAGCCACCGCGACTTCCAGCCGGTGCTCCACCTGGTTGC GCTCAACAGCCCCCTGTCAGGCGGCATGCGGGGCATCCGCGGGGCCGACTTCCAGTGCTTCCAGCAGGCG CGGGCCGTGGGGCTGGCGGGCACCTTCCGCGCCTTCCTGTCCTCGCGCCTGCAGGACCTGTACAGCATCG TGCGCCGTGCCGACCGCGCAGCCGTGCCCATCGTCAACCTCAAGGACGAGCTGCTGTTTCCCAGCTGGGA GGCTCTGTTCTCAGGCTCTGAGGGTCCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGACGGCAAGGAC GTCCTGAGGCACCCCACCTGGCCCCAGAAGAGCGTGTGGCATGGCTCGGACCCCAACGGGCGCAGGCTGA CCGAGAGCTACTGTGAGACGTGGCGGACGGAGGCTCCCTCGGCCACGGGCCAGGCCTCCTCGCTGCTGGG GGGCAGGCTCCTGGGGCAGAGTGCCGCGAGCTGCCATCACGCCTACATCGTGCTCTGCATTGAGAACAGC TTCATGACTGCCTCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209465 representing NM_130445
Red=Cloning site Green=Tags(s) MAPRCPWPWPRRRRLLDVLAPLVLLLGVRAASAEPERISEEVGLLQLLGDPPPQQVTQTDDPDVGLAYVF GPDANSGQVARYHFPSLFFRDFSLLFHIRPATEGPGVLFAITDSAQAMVLLGVKLSGVQDGHQDISLLYT EPGAGQTHTAASFRLPAFVGQWTHLALSVAGGFVALYVDCEEFQRMPLARSSRGLELEPGAGLFVAQAGG ADPDKFQGVIAELKVRRDPQVSPMHCLDEEGDDSDGASGDSGSGLGDARELLREETGAALKPRLPAPPPV TTPPLAGGSSTEDSRSEEVEEQTTVASLGAQTLPGSDSVSTWDGSVRTPGGRVKEGGLKGQKGEPGVPGP PGRAGPPGSPCLPGPPGLPCPVSPLGPAGPALQTVPGPQGPPGPPGRDGTPGRDGEPGDPGEDGKPGDTG PQGFPGTPGDVGPKGDKGDPGVGERGPPGPQGPPGPPGPSFRHDKLTFIDMEGSGFGGDLEALRGPRGFP GPPGPPGVPGLPGEPGRFGVNSSDVPGPAGLPGVPGREGPPGFPGLPGPPGPPGREGPPGRTGQKGSLGE AGAPGHKGSKGAPGPAGARGESGLAGAPGPAGPPGPPGPPGPPGPGLPAGFDDMEGSGGPFWSTARSADG PQGPPGLPGLKGDPGVPGLPGAKGEVGADGVPGFPGLPGREGIAGPQGPKGDRGSRGEKGDPGKDGVGQP GLPGPPGPPGPVVYVSEQDGSVLSVPGPEGRPGFAGFPGPAGPKGNLGSKGERGSPGPKGEKGEPGSIFS PDGGALGPAQKGAKGEPGFRGPPGPYGRPGYKGEIGFPGRPGRPGMNGLKGEKGEPGDASLGFGMRGMPG PPGPPGPPGPPGTPVYDSNVFAESSRPGPPGLPGNQGPPGPKGAKGEVGPPGPPGQFPFDFLQLEAEMKG EKGDRGDAGQKGERGEPGGGGFFGSSLPGPPGPPGPRGYPGIPGPKGESIRGQPGPPGPQGPPGIGYEGR QGPPGPPGPPGPPSFPGPHRQTISVPGPPGPPGPPGPPGTMGASSGVRLWATRQAMLGQVHEVPEGWLIF VAEQEELYVRVQNGFRKVQLEARTPLPRGTDNEVAALQPPVVQLHDSNPYPRREHPHPTARPWRADDILA SPPRLPEPQPYPGAPHHSSYVHLRPARPTSPPAHSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQA RAVGLAGTFRAFLSSRLQDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKD VLRHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCHHAYIVLCIENS FMTASK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130445 |
| ORF Size | 4008 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130445.2, NP_569712.2 |
| RefSeq Size | 5398 bp |
| RefSeq ORF | 4011 bp |
| Locus ID | 80781 |
| UniProt ID | P39060 |
| Gene Summary | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
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