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Endostatin (COL18A1) (NM_130445) Human Untagged Clone
CAT＃: SC305890
COL18A1 (untagged)-Human collagen, type XVIII, alpha 1 (COL18A1), transcript variant 2
CNY 13,720.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KNO; KNO1; KS |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_130445 edited
ATGGCGCCGAGGTGCCCCTGGCCATGGCCGCGGCGGCGGCGCCTCCTGGACGTGCTCGCG CCCCTGGTCCTGCTGCTCGGGGTCCGCGCGGCCTCCGCGGAGCCAGAGCGCATCAGCGAG GAGGTGGGGCTGCTGCAGCTCCTTGGGGACCCCCCGCCCCAGCAGGTCACCCAGACGGAT GACCCCGACGTCGGGCTGGCCTACGTCTTTGGGCCAGATGCCAACAGTGGCCAAGTGGCC CGGTACCACTTCCCCAGCCTCTTCTTCCGTGACTTCTCACTGCTGTTCCACATCCGGCCA GCCACAGAGGGCCCAGGGGTGCTGTTCGCCATCACGGACTCGGCGCAGGCCATGGTCTTG CTGGGCGTGAAGCTCTCTGGGGTGCAGGACGGGCACCAGGACATCTCCCTGCTCTACACA GAACCAGGTGCAGGCCAGACCCACACAGCCGCCAGCTTCCGGCTCCCCGCCTTCGTCGGC CAGTGGACACACTTAGCCCTCAGTGTGGCAGGTGGCTTTGTGGCCCTCTACGTGGACTGT GAGGAGTTCCAGAGAATGCCGCTTGCTCGGTCCTCACGGGGCCTGGAGCTGGAGCCTGGC GCCGGGCTCTTCGTGGCTCAGGCGGGGGGAGCGGACCCTGACAAGTTCCAGGGGGTGATC GCTGAGCTGAAGGTGCGCAGGGACCCCCAGGTGAGCCCCATGCACTGCCTGGACGAGGAA GGCGATGACTCAGATGGGGCATCCGGAGACTCTGGCAGCGGGCTCGGGGACGCCCGGGAG CTTCTCAGGGAGGAGACGGGCGCGGCCCTAAAACCCAGGCTCCCCGCGCCACCCCCCGTC ACCACGCCACCCTTGGCTGGAGGCAGCAGCACGGAAGATTCCAGAAGTGAAGAAGTCGAG GAGCAGACCACGGTGGCTTCGTTAGGAGCTCAGACACTTCCTGGCTCAGATTCTGTCTCC ACGTGGGACGGGAGTGTCCGGACCCCTGGGGGCCGCGTGAAAGAGGGCGGCCTGAAGGGG CAGAAAGGGGAGCCAGGTGTTCCGGGCCCACCTGGCCGGGCAGGCCCCCCAGGATCCCCA TGCCTACCTGGTCCCCCGGGTCTCCCGTGCCCAGTGAGTCCCCTGGGTCCTGCAGGCCCA GCGTTGCAAACTGTCCCCGGACCACAAGGACCCCCAGGGCCTCCGGGGAGGGACGGCACC CCTGGAAGGGACGGCGAGCCGGGCGACCCCGGTGAAGACGGAAAGCCGGGCGACACCGGG CCACAAGGCTTCCCCGGGACTCCAGGGGACGTAGGTCCCAAGGGCGACAAGGGAGACCCT GGGGTTGGAGAGAGAGGGCCCCCAGGACCCCAAGGGCCTCCAGGGCCCCCAGGACCCTCC TTCAGACACGACAAGCTGACCTTCATTGACATGGAGGGATCTGGCTTCGGGGGCGATCTG GAGGCCCTGCGGGGTCCTCGAGGCTTCCCTGGACCTCCCGGACCCCCCGGTGTCCCAGGC CTGCCCGGCGAGCCAGGCCGCTTTGGGGTGAACAGCTCCGACGTCCCAGGACCCGCCGGC CTTCCTGGTGTGCCTGGGCGCGAGGGTCCCCCCGGGTTTCCTGGCCTCCCGGGACCCCCA GGCCCTCCGGGAAGAGAGGGGCCCCCAGGAAGGACTGGGCAGAAAGGCAGCCTGGGTGAA GCAGGCGCCCCAGGACATAAGGGGAGCAAGGGAGCCCCCGGTCCTGCTGGTGCTCGTGGG GAGAGCGGCCTGGCAGGAGCCCCCGGACCTGCTGGACCACCAGGCCCCCCTGGGCCCCCT GGGCCCCCAGGACCAGGACTCCCCGCTGGATTTGATGACATGGAAGGCTCCGGGGGGCCC TTCTGGTCAACAGCCCGAAGCGCTGATGGGCCACAGGGACCTCCCGGCCTGCCGGGACTT AAGGGGGATCCTGGCGTGCCTGGGCTGCCGGGGGCGAAGGGAGAAGTTGGAGCAGATGGA GTCCCCGGGTTCCCCGGCCTCCCTGGCAGAGAGGGCATTGCTGGGCCCCAGGGGCCAAAG GGAGACAGAGGCAGCCGGGGAGAAAAGGGAGATCCAGGGAAGGACGGAGTCGGGCAGCCG GGCCTCCCTGGCCCCCCCGGACCCCCGGGACCTGTGGTCTACGTGTCGGAGCAGGACGGA TCCGTCCTGAGCGTGCCGGGACCTGAGGGCCGGCCGGGTTTCGCAGGCTTTCCCGGACCT GCAGGACCCAAGGGCAACCTGGGCTCTAAGGGCGAACGAGGCTCCCCGGGACCCAAGGGT GAGAAGGGTGAACCGGGCAGCATCTTCAGCCCCGACGGCGGTGCCCTGGGCCCTGCCCAG AAAGGAGCCAAGGGAGAGCCGGGCTTCCGAGGACCCCCGGGTCCATACGGACGGCCGGGG TACAAGGGAGAGATTGGCTTTCCTGGACGGCCGGGTCGCCCCGGGATGAACGGATTGAAA GGAGAGAAAGGGGAGCCGGGAGATGCCAGCCTTGGATTTGGCATGAGGGGAATGCCCGGC CCCCCAGGACCTCCAGGGCCCCCAGGCCCTCCAGGGACTCCTGTTTACGACAGCAATGTG TTTGCTGAGTCCAGCCGCCCCGGGCCTCCAGGATTGCCAGGGAATCAGGGCCCTCCAGGA CCCAAGGGCGCCAAAGGAGAAGTGGGCCCCCCCGGACCACCAGGGCAGTTTCCGTTTGAC TTTCTTCAGTTGGAGGCTGAAATGAAGGGGGAGAAGGGAGACCGAGGTGATGCAGGACAG AAAGGCGAAAGGGGGGAGCCCGGGGGCGGCGGTTTCTTCGGCTCCAGCCTGCCCGGCCCC CCCGGCCCCCCAGGCCCACGTGGCTACCCTGGGATTCCAGGTCCCAAGGGAGAGAGCATC CGGGGCCAGCCCGGCCCACCTGGACCTCAGGGACCCCCCGGCATCGGCTACGAGGGGCGC CAGGGCCCTCCCGGCCCCCCAGGCCCCCCAGGGCCCCCTTCATTTCCTGGCCCTCACAGG CAGACTATCAGCGTTCCCGGCCCTCCGGGCCCCCCTGGGCCCCCTGGGCCCCCTGGAACC ATGGGCGCCTCCTCAGGGGTGAGGCTCTGGGCTACACGCCAGGCCATGCTGGGCCAGGTG CACGAGGTTCCCGAGGGCTGGCTCATCTTCGTGGCCGAGCAGGAGGAGCTCTACGTCCGC GTGCAGAACGGGTTCCGGAAGGTCCAGCTGGAGGCCCGGACACCACTCCCACGAGGGACG GACAATGAAGTGGCCGCCTTGCAGCCCCCCGTGGTGCAGCTGCACGACAGCAACCCCTAC CCGCGGCGGGAGCACCCCCACCCCACCGCGCGGCCCTGGCGGGCAGATGACATCCTGGCC AGCCCCCCTCGCCTGCCCGAGCCCCAGCCCTACCCCGGAGCCCCGCACCACAGCTCCTAC GTGCACCTGCGGCCGGCGCGACCCACAAGCCCACCCGCCCACAGCCACCGCGACTTCCAG CCGGTGCTCCACCTGGTTGCGCTCAACAGCCCCCTGTCAGGCGGCATGCGGGGCATCCGC GGGGCCGACTTCCAGTGCTTCCAGCAGGCGCGGGCCGTGGGGCTGGCGGGCACCTTCCGC GCCTTCCTGTCCTCGCGCCTGCAGGACCTGTACAGCATCGTGCGCCGTGCCGACCGCGCA GCCGTGCCCATCGTCAACCTCAAGGACGAGCTGCTGTTTCCCAGCTGGGAGGCTCTGTTC TCAGGCTCTGAGGGTCCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGACGGCAAGGAC GTCCTGAGGCACCCCACCTGGCCCCAGAAGAGCGTGTGGCATGGCTCGGACCCCAACGGG CGCAGGCTGACCGAGAGCTACTGTGAGACGTGGCGGACGGAGGCTCCCTCGGCCACGGGC CAGGCCTCCTCGCTGCTGGGGGGCAGGCTCCTGGGGCAGAGTGCCGCGAGCTGCCATCAC GCCTACATCGTGCTCTGCATTGAGAACAGCTTCATGACTGCCTCCAAGTAG |
| Restriction Sites | Please inquire |
| ACCN | NM_130445 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_130445.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130445.1, NP_569712.1 |
| RefSeq Size | 5398 bp |
| RefSeq ORF | 4011 bp |
| Locus ID | 80781 |
| UniProt ID | P39060 |
| Gene Summary | This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2, also known as NCI-303) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 3. The encoded isoform (2) has a distinct and shorter N-terminus compared to isoform 3. |
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