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Metabotropic Glutamate Receptor 3 (GRM3) (NM_000840) Human Tagged ORF Clone
CAT＃: RG207379
- TrueORF®
GRM3 (tGFP-tagged) - Human glutamate receptor, metabotropic 3 (GRM3)
ORF Plasmid: DDK
"NM_000840" in other vectors (6)
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CNY 8,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GLUR3; GPRC1C; mGlu3; MGLUR3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207379 representing NM_000840
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATGTTGACAAGACTGCAAGTTCTTACCTTAGCTTTGTTTTCAAAGGGATTTTTACTCTCTTTAG GGGACCATAACTTTCTAAGGAGAGAGATTAAAATAGAAGGTGACCTTGTTTTAGGGGGCCTGTTTCCTAT TAACGAAAAAGGCACTGGAACTGAAGAATGTGGGCGAATCAATGAAGACCGAGGGATTCAACGCCTGGAA GCCATGTTGTTTGCTATTGATGAAATCAACAAAGATGATTACTTGCTACCAGGAGTGAAGTTGGGTGTTC ACATTTTTGATACATGTTCAAGGGATACCTATGCATTGGAGCAATCACTGGAGTTTGTCAGGGCATCTTT GACAAAAGTGGATGAAGCTGAGTATATGTGTCCTGATGGATCCTATGCCATTCAAGAAAACATCCCACTT CTCATTGCAGGGGTCATTGGTGGCTCTTATAGCAGTGTTTCCATACAGGTGGCAAACCTGCTGCGGCTCT TCCAGATCCCTCAGATCAGCTACGCATCCACCAGCGCCAAACTCAGTGATAAGTCGCGCTATGATTACTT TGCCAGGACCGTGCCCCCCGACTTCTACCAGGCCAAAGCCATGGCTGAGATCTTGCGCTTCTTCAACTGG ACCTACGTGTCCACAGTAGCCTCCGAGGGTGATTACGGGGAGACAGGGATCGAGGCCTTCGAGCAGGAAG CCCGCCTGCGCAACATCTGCATCGCTACGGCGGAGAAGGTGGGCCGCTCCAACATCCGCAAGTCCTACGA CAGCGTGATCCGAGAACTGTTGCAGAAGCCCAACGCGCGCGTCGTGGTCCTCTTCATGCGCAGCGACGAC TCGCGGGAGCTCATTGCAGCCGCCAGCCGCGCCAATGCCTCCTTCACCTGGGTGGCCAGCGACGGCTGGG GCGCGCAGGAGAGCATCATCAAGGGCAGCGAGCATGTGGCCTACGGCGCCATCACCCTGGAGCTGGCCTC CCAGCCTGTCCGCCAGTTCGACCGCTACTTCCAGAGCCTCAACCCCTACAACAACCACCGCAACCCCTGG TTCCGGGACTTCTGGGAGCAAAAGTTTCAGTGCAGCCTCCAGAACAAACGCAACCACAGGCGCGTCTGCG ACAAGCACCTGGCCATCGACAGCAGCAACTACGAGCAAGAGTCCAAGATCATGTTTGTGGTGAACGCGGT GTATGCCATGGCCCACGCTTTGCACAAAATGCAGCGCACCCTCTGTCCCAACACTACCAAGCTTTGTGAT GCTATGAAGATCCTGGATGGGAAGAAGTTGTACAAGGATTACTTGCTGAAAATCAACTTCACGGCTCCAT TCAACCCAAATAAAGATGCAGATAGCATAGTCAAGTTTGACACTTTTGGAGATGGAATGGGGCGATACAA CGTGTTCAATTTCCAAAATGTAGGTGGAAAGTATTCCTACTTGAAAGTTGGTCACTGGGCAGAAACCTTA TCGCTAGATGTCAACTCTATCCACTGGTCCCGGAACTCAGTCCCCACTTCCCAGTGCAGCGACCCCTGTG CCCCCAATGAAATGAAGAATATGCAACCAGGGGATGTCTGCTGCTGGATTTGCATCCCCTGTGAACCCTA CGAATACCTGGCTGATGAGTTTACCTGTATGGATTGTGGGTCTGGACAGTGGCCCACTGCAGACCTAACT GGATGCTATGACCTTCCTGAGGACTACATCAGGTGGGAAGACGCCTGGGCCATTGGCCCAGTCACCATTG CCTGTCTGGGTTTTATGTGTACATGCATGGTTGTAACTGTTTTTATCAAGCACAACAACACACCCTTGGT CAAAGCATCGGGCCGAGAACTCTGCTACATCTTATTGTTTGGGGTTGGCCTGTCATACTGCATGACATTC TTCTTCATTGCCAAGCCATCACCAGTCATCTGTGCATTGCGCCGACTCGGGCTGGGGAGTTCCTTCGCTA TCTGTTACTCAGCCCTGCTGACCAAGACAAACTGCATTGCCCGCATCTTCGATGGGGTCAAGAATGGCGC TCAGAGGCCAAAATTCATCAGCCCCAGTTCTCAGGTTTTCATCTGCCTGGGTCTGATCCTGGTGCAAATT GTGATGGTGTCTGTGTGGCTCATCCTGGAGGCCCCAGGCACCAGGAGGTATACCCTTGCAGAGAAGCGGG AAACAGTCATCCTAAAATGCAATGTCAAAGATTCCAGCATGTTGATCTCTCTTACCTACGATGTGATCCT GGTGATCTTATGCACTGTGTACGCCTTCAAAACGCGGAAGTGCCCAGAAAATTTCAACGAAGCTAAGTTC ATAGGTTTTACCATGTACACCACGTGCATCATCTGGTTGGCCTTCCTCCCTATATTTTATGTGACATCAA GTGACTACAGAGTGCAGACGACAACCATGTGCATCTCTGTCAGCCTGAGTGGCTTTGTGGTCTTGGGCTG TTTGTTTGCACCCAAGGTTCACATCATCCTGTTTCAACCCCAGAAGAATGTTGTCACACACAGACTGCAC CTCAACAGGTTCAGTGTCAGTGGAACTGGGACCACATACTCTCAGTCCTCTGCAAGCACGTATGTGCCAA CGGTGTGCAATGGGCGGGAAGTCCTCGACTCCACCACCTCATCTCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207379 representing NM_000840
Red=Cloning site Green=Tags(s) MKMLTRLQVLTLALFSKGFLLSLGDHNFLRREIKIEGDLVLGGLFPINEKGTGTEECGRINEDRGIQRLE AMLFAIDEINKDDYLLPGVKLGVHIFDTCSRDTYALEQSLEFVRASLTKVDEAEYMCPDGSYAIQENIPL LIAGVIGGSYSSVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFYQAKAMAEILRFFNW TYVSTVASEGDYGETGIEAFEQEARLRNICIATAEKVGRSNIRKSYDSVIRELLQKPNARVVVLFMRSDD SRELIAAASRANASFTWVASDGWGAQESIIKGSEHVAYGAITLELASQPVRQFDRYFQSLNPYNNHRNPW FRDFWEQKFQCSLQNKRNHRRVCDKHLAIDSSNYEQESKIMFVVNAVYAMAHALHKMQRTLCPNTTKLCD AMKILDGKKLYKDYLLKINFTAPFNPNKDADSIVKFDTFGDGMGRYNVFNFQNVGGKYSYLKVGHWAETL SLDVNSIHWSRNSVPTSQCSDPCAPNEMKNMQPGDVCCWICIPCEPYEYLADEFTCMDCGSGQWPTADLT GCYDLPEDYIRWEDAWAIGPVTIACLGFMCTCMVVTVFIKHNNTPLVKASGRELCYILLFGVGLSYCMTF FFIAKPSPVICALRRLGLGSSFAICYSALLTKTNCIARIFDGVKNGAQRPKFISPSSQVFICLGLILVQI VMVSVWLILEAPGTRRYTLAEKRETVILKCNVKDSSMLISLTYDVILVILCTVYAFKTRKCPENFNEAKF IGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCISVSLSGFVVLGCLFAPKVHIILFQPQKNVVTHRLH LNRFSVSGTGTTYSQSSASTYVPTVCNGREVLDSTTSSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000840 |
| ORF Size | 2637 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000840.2, NP_000831.2 |
| RefSeq Size | 4260 bp |
| RefSeq ORF | 2640 bp |
| Locus ID | 2913 |
| UniProt ID | Q14832 |
| Domains | 7tm_3, ANF_receptor |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Gene Summary | L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207379 | GRM3 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 3 (GRM3) |
CNY 6,440.00 |
|
| RC207379L1 | Lenti ORF clone of Human glutamate receptor, metabotropic 3 (GRM3), Myc-DDK-tagged |
CNY 8,840.00 |
|
| RC207379L2 | Lenti ORF clone of Human glutamate receptor, metabotropic 3 (GRM3), mGFP tagged |
CNY 9,030.00 |
|
| RC207379L3 | Lenti ORF clone of Human glutamate receptor, metabotropic 3 (GRM3), Myc-DDK-tagged |
CNY 8,840.00 |
|
| RC207379L4 | Lenti ORF clone of Human glutamate receptor, metabotropic 3 (GRM3), mGFP tagged |
CNY 8,840.00 |
|
| SC125500 | GRM3 (untagged)-Human glutamate receptor, metabotropic 3 (GRM3) |
CNY 6,448.00 |
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