PHKA2 (NM_000292) Human Tagged ORF Clone
CAT#: RG204185
- TrueORF®
PHKA2 (tGFP-tagged) - Human phosphorylase kinase, alpha 2 (liver) (PHKA2)
ORF Plasmid: DDK
"NM_000292" in other vectors (4)
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CNY 11,590.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GSD9A; PHK; PYK; PYKL; XLG; XLG2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204185 representing NM_000292
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAGCAGGAGCAATTCCGGGGTCCGCTTGGACGGGTACGCGCGGCTGGTGCAGCAAACCATCCTGT GTTACCAGAATCCCGTCACGGGGCTGCTGTCAGCCAGCCATGAGCAGAAGGATGCCTGGGTGCGGGATAA CATCTACAGTATCCTGGCCGTGTGGGGCCTGGGCATGGCCTACCGTAAGAATGCAGACCGCGATGAGGAC AAGGCCAAGGCCTACGAGCTGGAGCAGAACGTGGTGAAGCTGATGCGAGGTCTTCTCCAGTGCATGATGA GACAGGTGGCCAAAGTGGAGAAGTTCAAACACACTCAGAGCACCAAGGACAGCCTGCACGCCAAGTACAA CACCGCCACCTGTGGCACGGTGGTGGGCGACGACCAGTGGGGCCACCTCCAGGTGGATGCCACCTCTCTC TTCCTCCTGTTCCTGGCCCAGATGACCGCCTCAGGCTTACGTATCATTTTCACTCTCGATGAGGTGGCCT TCATACAGAATCTTGTCTTTTACATAGAAGCTGCATATAAAGTCGCTGATTATGGAATGTGGGAGCGTGG AGATAAGACTAATCAGGGCATCCCGGAATTGAATGCAAGCTCCGTAGGAATGGCCAAGGCAGCTCTTGAG GCAATTGATGAACTGGACCTTTTTGGAGCCCATGGAGGACGCAAGTCAGTGATTCATGTTCTGCCAGATG AGGTCGAGCACTGCCAGTCTATTCTGTTCTCCATGCTGCCAAGAGCGTCGACATCTAAAGAAATTGATGC TGGACTTCTTTCCATTATTTCCTTCCCGGCCTTTGCAGTGGAAGATGTAAACCTTGTAAATGTGACCAAA AATGAAATTATTTCTAAGCTCCAGGGGCGTTATGGATGCTGTCGCTTCCTTCGAGATGGTTATAAAACTC CAAGAGAGGACCCTAATCGACTGCATTATGACCCTGCTGAACTCAAGCTCTTCGAAAACATTGAATGTGA GTGGCCTGTGTTTTGGACATATTTTATAATAGATGGAGTCTTCAGTGGTGATGCTGTTCAGGTCCAAGAA TACCGAGAGGCCCTGGAGGGAATACTCATCAGAGGCAAGAATGGGATCCGCCTGGTGCCTGAACTCTACG CTGTCCCGCCTAACAAGGTAGATGAAGAGTACAAGAATCCTCACACAGTAGACCGAGTTCCTATGGGGAA GGTGCCTCATCTGTGGGGCCAATCCTTGTACATCCTCAGCTCGCTGTTGGCAGAGGGATTCCTTGCCGCT GGTGAAATCGATCCCTTAAATAGAAGATTTTCCACTTCAGTCAAACCTGATGTTGTAGTACAAGTTACTG TTTTGGCAGAAAACAATCACATTAAGGACTTATTGAGGAAACACGGGGTGAACGTCCAGAGTATCGCGGA CATTCATCCAATTCAAGTCCAGCCGGGCCGGATTCTTAGTCACATATATGCCAAGCTTGGACGGAATAAG AATATGAATTTGAGTGGGCGACCGTATCGACATATTGGTGTCCTTGGAACCTCTAAACTATATGTGATTA GGAACCAAATCTTTACTTTTACACCCCAGTTCACCGACGAGCATCACTTCTACCTGGCCCTCGACAATGA GATGATCGTGGAGATGCTAAGGATCGAGCTGGCCTACCTGTGCACCTGCTGGAGGATGACGGGCAGACCC ACACTCACCTTCCCCATCAGTCGCACCATGCTCACAAATGATGGCTCAGACATTCATTCTGCTGTGCTCT CCACAATTAGAAAACTAGAGGATGGATATTTTGGAGGAGCCAGAGTAAAATTAGGGAACCTTTCGGAATT TCTCACCACATCGTTCTACACATATCTGACTTTTCTGGATCCAGACTGTGATGAGAAGTTGTTTGACAAT GCCAGCGAAGGGACTTTCAGTCCTGATAGTGATTCAGATTTGGTAGGATATCTGGAAGACACCTGTAATC AAGAAAGCCAAGACGAACTTGACCATTATATCAACCACCTTCTGCAAAGCACATCGTTGAGGTCCTATCT GCCTCCTCTTTGTAAGAACACAGAAGACCGCCATGTCTTCAGTGCTATCCACTCCACGCGGGACATACTT TCTGTGATGGCAAAAGCAAAGGGTTTGGAAGTTCCATTTGTTCCCATGACTTTGCCGACTAAAGTTCTAA GTGCCCACCGTAAATCACTGAATCTTGTTGATTCTCCTCAGCCACTCCTAGAAAAGGTTCCTGAAAGTGA CTTTCAGTGGCCCAGAGATGACCATAGTGACGTGGACTGTGAGAAGCTGGTTGAGCAGCTAAAAGATTGT TCGAACCTACAGGACCAAGCAGACATTCTGTACATTCTTTATGTCATAAAGGGTCCCAGCTGGGACACAA ATCTCTCTGGACAGCACGGGGTCACCGTTCAAAACCTTCTTGGTGAGCTCTATGGGAAAGCCGGCTTGAA CCAGGAGTGGGGTCTGATTCGCTACATCTCAGGCCTTCTCAGGAAGAAAGTGGAGGTCCTGGCTGAGGCC TGCACAGACCTGCTTTCGCACCAGAAGCAGCTCACCGTGGGCCTGCCGCCCGAGCCCCGGGAGAAGATCA TCTCTGCGCCCCTTCCCCCAGAGGAGCTCACAAAACTCATCTACGAGGCCAGTGGGCAGGACATCAGCAT TGCCGTCCTCACGCAGGAGATTGTGGTTTACCTGGCCATGTATGTCAGGGCGCAGCCCAGCCTCTTTGTG GAGATGCTGAGACTCCGGATTGGACTGATCATTCAGGTGATGGCCACGGAGCTGGCACGGAGCCTGAACT GCTCAGGAGAAGAGGCTTCTGAAAGTTTGATGAACCTCAGCCCTTTCGATATGAAAAATCTCCTGCACCA TATTCTAAGTGGGAAAGAGTTTGGCGTTGAAAGAAGTGTGCGCCCTATCCACTCCTCCACATCCAGCCCT ACCATCTCCATCCACGAGGTGGGCCATACCGGAGTCACCAAAACTGAGAGGAGTGGCATTAACAGACTGA GGAGTGAAATGAAACAGATGACTAGGCGGTTTAGTGCTGATGAACAGTTCTTTTCTGTGGGCCAGGCCGC GTCCAGCAGTGCGCATTCCTCCAAGTCTGCGAGGTCCAGCACCCCATCCTCGCCCACTGGCACGTCATCC TCAGACTCGGGAGGACATCACATCGGCTGGGGTGAGCGGCAGGGCCAGTGGCTGCGCAGGAGAAGGCTGG ATGGGGCCATCAACAGGGTCCCCGTGGGATTCTACCAGAGGGTGTGGAAGATCCTCCAGAAGTGCCACGG TCTCTCCATCGATGGTTATGTCCTCCCATCCTCGACGACCCGAGAGATGACCCCGCATGAGATCAAGTTT GCTGTCCATGTCGAATCGGTGCTGAACCGCGTGCCGCAGCCCGAGTACCGGCAGCTGCTGGTGGAAGCCA TCATGGTGCTGACGCTGCTCTCGGACACGGAGATGACCAGCATCGGGGGCATCATCCACGTGGACCAGAT CGTGCAGATGGCCAGTCAGCTGTTCTTGCAGGACCAGGTGTCAATTGGTGCCATGGACACCCTGGAGAAA GACCAAGCCACAGGAATCTGCCACTTCTTTTATGACAGCGCTCCGAGTGGGGCTTATGGGACGATGACCT ACCTAACAAGAGCAGTGGCTTCTTATTTGCAGGAATTGTTGCCCAATTCGGGCTGCCAGATGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204185 representing NM_000292
Red=Cloning site Green=Tags(s) MRSRSNSGVRLDGYARLVQQTILCYQNPVTGLLSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDED KAKAYELEQNVVKLMRGLLQCMMRQVAKVEKFKHTQSTKDSLHAKYNTATCGTVVGDDQWGHLQVDATSL FLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALE AIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTK NEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFSGDAVQVQE YREALEGILIRGKNGIRLVPELYAVPPNKVDEEYKNPHTVDRVPMGKVPHLWGQSLYILSSLLAEGFLAA GEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKDLLRKHGVNVQSIADIHPIQVQPGRILSHIYAKLGRNK NMNLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDEHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRP TLTFPISRTMLTNDGSDIHSAVLSTIRKLEDGYFGGARVKLGNLSEFLTTSFYTYLTFLDPDCDEKLFDN ASEGTFSPDSDSDLVGYLEDTCNQESQDELDHYINHLLQSTSLRSYLPPLCKNTEDRHVFSAIHSTRDIL SVMAKAKGLEVPFVPMTLPTKVLSAHRKSLNLVDSPQPLLEKVPESDFQWPRDDHSDVDCEKLVEQLKDC SNLQDQADILYILYVIKGPSWDTNLSGQHGVTVQNLLGELYGKAGLNQEWGLIRYISGLLRKKVEVLAEA CTDLLSHQKQLTVGLPPEPREKIISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFV EMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSVRPIHSSTSSP TISIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQFFSVGQAASSSAHSSKSARSSTPSSPTGTSS SDSGGHHIGWGERQGQWLRRRRLDGAINRVPVGFYQRVWKILQKCHGLSIDGYVLPSSTTREMTPHEIKF AVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMTSIGGIIHVDQIVQMASQLFLQDQVSIGAMDTLEK DQATGICHFFYDSAPSGAYGTMTYLTRAVASYLQELLPNSGCQMQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000292 |
ORF Size | 3705 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000292.1, NP_000283.1 |
RefSeq Size | 4566 bp |
RefSeq ORF | 3708 bp |
Locus ID | 5256 |
UniProt ID | P46019 |
Protein Families | Druggable Genome |
Protein Pathways | Calcium signaling pathway, Insulin signaling pathway |
Gene Summary | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, which are encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9A, also known as X-linked liver glycogenosis. Alternatively spliced transcript variants have been reported, but the full-length nature of these variants has not been determined.[provided by RefSeq, Feb 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204185 | PHKA2 (Myc-DDK-tagged)-Human phosphorylase kinase, alpha 2 (liver) (PHKA2) |
CNY 8,600.00 |
|
RC204185L3 | Lenti ORF clone of Human phosphorylase kinase, alpha 2 (liver) (PHKA2), Myc-DDK-tagged |
CNY 12,450.00 |
|
RC204185L4 | Lenti ORF clone of Human phosphorylase kinase, alpha 2 (liver) (PHKA2), mGFP tagged |
CNY 12,450.00 |
|
SC319895 | PHKA2 (untagged)-Human phosphorylase kinase, alpha 2 (liver) (PHKA2) |
CNY 8,608.00 |