RNF183 (NM_145051) Human Tagged ORF Clone
CAT#: RG201440
- TrueORF®
RNF183 (tGFP-tagged) - Human ring finger protein 183 (RNF183)
ORF Plasmid: DDK
"NM_145051" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG201440 representing NM_145051
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGCAGCAGGGCCGGGAGCTTGAGGCTGAGTGCCCCGTCTGCTGGAACCCCTTCAACAACACGT TCCATACCCCCAAAATGCTGGATTGCTGCCACTCCTTCTGCGTGGAATGTCTGGCCCACCTCAGCCTTGT GACTCCAGCCCGGCGCCGCCTGCTGTGCCCACTCTGTCGCCAGCCCACAGTGCTGGCCTCAGGGCAGCCT GTCACTGACTTGCCCACGGACACTGCCATGCTCACCCTGCTCCGCCTGGAGCCCCACCATGTCATCCTGG AAGGCCATCAGCTGTGCCTCAAGGACCAGCCCAAGAGCCGCTACTTCCTGCGCCAGCCTCGAGTCTACAC GCTGGACCTTGGCCCCCAGCCTGGGGGCCAGACTGGGCCGCCCCCAGACACGGCCTCTGCCACCGTGTCT ACGCCCATCCTCATCCCCAGCCACCACTCTTTGAGGGAGTGTTTCCGCAACCCTCAGTTCCGCATCTTTG CCTACCTGATGGCCGTCATCCTCAGTGTCACTCTGTTGCTCATATTCTCCATCTTTTGGACCAAGCAGTT CCTTTGGGGTGTGGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG201440 representing NM_145051
Red=Cloning site Green=Tags(s) MAEQQGRELEAECPVCWNPFNNTFHTPKMLDCCHSFCVECLAHLSLVTPARRRLLCPLCRQPTVLASGQP VTDLPTDTAMLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDLGPQPGGQTGPPPDTASATVS TPILIPSHHSLRECFRNPQFRIFAYLMAVILSVTLLLIFSIFWTKQFLWGVG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145051 |
ORF Size | 576 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145051.2, NP_659488.1 |
RefSeq Size | 1265 bp |
RefSeq ORF | 579 bp |
Locus ID | 138065 |
UniProt ID | Q96D59 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | Acts as a E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:29507230). Triggers apoptosis in response to prolonged ER stress by mediating the polyubiquitination and subsequent proteasomal degradation of BCL2L1 (PubMed:29507230). May collaborate with FATE1 to restrain BIK protein levels thus regulating apoptotic signaling (PubMed:26567849).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201440 | RNF183 (Myc-DDK-tagged)-Human ring finger protein 183 (RNF183) |
CNY 2,400.00 |
|
RC201440L1 | Lenti ORF clone of Human ring finger protein 183 (RNF183), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC201440L2 | Lenti ORF clone of Human ring finger protein 183 (RNF183), mGFP tagged |
CNY 5,890.00 |
|
RC201440L3 | Lenti ORF clone of Human ring finger protein 183 (RNF183), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201440L4 | Lenti ORF clone of Human ring finger protein 183 (RNF183), mGFP tagged |
CNY 5,890.00 |
|
SC123208 | RNF183 (untagged)-Human ring finger protein 183 (RNF183) |
CNY 2,400.00 |