Plakophilin 3 (PKP3) (NM_001303029) Human Tagged ORF Clone
CAT#: RC239617
- TrueORF®
PKP3 (myc-DDK-tagged) - Human plakophilin 3 (PKP3), transcript variant 2
ORF Plasmid: tGFP
"NM_001303029" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239617 representing NM_001303029
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCCTGGACACCTCGGCCGTCAGCAGTGGCCTCTGGGATGAGCTGGGAGGCTGGCGGGATCCGGA CCACGAGCCGGCCTGAGGCCGGCGTGTGCTCCCTGGCGCTGCCCTCTGACCTGCAGCTGGACCGCCGGGG CGCCGAGGGGCCGGAGGCCGAGCGGCTGCGGGCAGCCCGCGTCCAGGAGCAGGTCCGCGCCCGCCTCTTG CAGCTGGGACAGCAGCCGCGGCACAACGGGGCCGCTGAGCCCGAGCCTGAGGCCGAGACTGCCAGAGGCA CATCCAGGGGGCAGTACCACACCCTGCAGGCTGGCTTCAGCTCTCGCTCTCAGGGCCTGAGTGGGGACAA GACCTCGGGCTTCCGGCCCATCGCCAAGCCGGCCTACAGCCCAGCCTCCTGGTCCTCCCGCTCCGCCGTG GATCTGAGCTGCAGTCGGAGGCTGAGTTCAGCCCACAACGGGGGCAGCGCCTTTGGGGCCGCTGGGTACG GGGGTGCCCAGCCCACCCCTCCCATGCCCACCAGGCCCGTGTCCTTCCATGAGCGCGGTGGGGTTGGGAG CCGGGCCGACTATGACACACTCTCCCTGCGCTCGCTGCGGCTGGGGCCCGGGGGCCTGGACGACCGCTAC AGCCTGGTGTCTGAGCAGCTGGAGCCCGCGGCCACCTCCACCTACAGGGCCTTTGCGTACGAGCGCCAGG CCAGCTCCAGCTCCAGCCGGGCAGGGGGGCTGGACTGGCCCGAGGCCACTGAGGTTTCCCCGAGCCGGAC CATCCGTGCCCCTGCCGTGCGGACCCTGCAGCGATTCCAGAGCAGCCACCGGAGCCGCGGGGTAGGCGGG GCAGTGCCGGGGGCCGTCCTGGAGCCAGTGGCTCGAGCGCCATCTGTGCGCAGCCTCAGCCTCAGCCTGG CTGACTCGGGCCACCTGCCGGACGTGCATGGGTTCAACAGCTACGGTAGCCACCGAACCCTGCAGAGACT CAGCAGCGGTTTTGATGACATTGACCTGCCCTCAGCAGTCAAGTACCTCATGGCTTCAGACCCCAACCTG CAGGTGCTGGGAGCGGCCTACATCCAGCACAAGTGCTACAGCGATGCAGCCGCCAAGAAGCAGGCCCGCA GCCTTCAGGCCGTGCCTAGGCTGGTGAAGCTCTTCAACCACGCCAACCAGGAAGTGCAGCGCCATGCCAC AGGTGCCATGCGCAACCTCATCTACGACAACGCTGACAACAAGCTGGCCCTGGTGGAGGAGAACGGGATC TTCGAGCTGCTGCGGACACTGCGGGAGCAGGATGATGAGCTTCGCAAAAATGTCACAGGGATCCTGTGGA ACCTTTCATCCAGCGACCACCTGAAGGACCGCCTGGCCAGAGACACGCTGGAGCAGCTCACAGACCTGGT GTTGAGCCCCCTGTCGGGGGCTGGGGGTCCCCCCCTCATCCAGCAGAACGCCTCGGAGGCAGAGATCTTC TACAACGCCACCGGCTTCCTCAGGAACCTCAGCTCAGCCTCTCAGGCCACTCGCCAGAAGATGCGGGAGT GCCACGGGCTGGTGGACGCCCTGGTCACCTCTATCAACCACGCCCTGGACGCGGGCAAATGCGAGGACAA GAGCGTGGAGAACGCGGTGTGCGTCCTGCGGAACCTGTCCTACCGCCTCTACGACGAGATGCCGCCGTCC GCGCTGCAGCGGCTGGAGGGTCGCGGCCGCAGGGACCTGGCGGGGGCGCCGCCGGGAGAGGTCGTGGGCT GCTTCACGCCGCAGAGCCGGCGGCTGCGCGAGCTGCCCCTCGCCGCCGATGCGCTCACCTTCGCGGAGGT GTCCAAGGACCCCAAGGGCCTCGAGTGGCTGTGGAGCCCCCAGATCGTGGGGCTGTACAACCGGCTGCTG CAGCGCTGCGAGCTCAACCGGCACACGACGGAGGCGGCCGCCGGGGCGCTGCAGAACATCACGGCAGGCG ACCGCAGGTGGGCGGGGGTGCTGAGCCGCCTGGCCCTGGAGCAGGAGCGTATTCTGAACCCCCTGCTAGA CCGTGTCAGGACCGCCGACCACCACCAGCTGCGCTCACTGACTGGCCTCATCCGAAACCTGTCTCGGAAC GCTAGGAACAAGGACGAGATGTCCACGAAGGTGGTGAGCCACCTGATCGAGAAGCTGCCGGGCAGCGTGG GTGAGAAGTCGCCCCCAGCCGAGGTGCTGGTCAACATCATAGCTGTGCTCAACAACCTGGTGGTGGCCAG CCCCATCGCTGCCCGAGACCTGCTGTATTTTGACGGACTCCGAAAGCTCATCTTCATCAAGAAGAAGCGG GACAGCCCCGACAGTGAGAAGTCCTCCCGGGCAGCATCCAGCCTCCTGGCCAACCTGTGGCAGTACAACA AGCTCCACCGTGACTTCCGGGCGAAGGGCTATCGGAAGGAGGACTTCCTGGGCCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239617 representing NM_001303029
Red=Cloning site Green=Tags(s) MESWTPRPSAVASGMSWEAGGIRTTSRPEAGVCSLALPSDLQLDRRGAEGPEAERLRAARVQEQVRARLL QLGQQPRHNGAAEPEPEAETARGTSRGQYHTLQAGFSSRSQGLSGDKTSGFRPIAKPAYSPASWSSRSAV DLSCSRRLSSAHNGGSAFGAAGYGGAQPTPPMPTRPVSFHERGGVGSRADYDTLSLRSLRLGPGGLDDRY SLVSEQLEPAATSTYRAFAYERQASSSSSRAGGLDWPEATEVSPSRTIRAPAVRTLQRFQSSHRSRGVGG AVPGAVLEPVARAPSVRSLSLSLADSGHLPDVHGFNSYGSHRTLQRLSSGFDDIDLPSAVKYLMASDPNL QVLGAAYIQHKCYSDAAAKKQARSLQAVPRLVKLFNHANQEVQRHATGAMRNLIYDNADNKLALVEENGI FELLRTLREQDDELRKNVTGILWNLSSSDHLKDRLARDTLEQLTDLVLSPLSGAGGPPLIQQNASEAEIF YNATGFLRNLSSASQATRQKMRECHGLVDALVTSINHALDAGKCEDKSVENAVCVLRNLSYRLYDEMPPS ALQRLEGRGRRDLAGAPPGEVVGCFTPQSRRLRELPLAADALTFAEVSKDPKGLEWLWSPQIVGLYNRLL QRCELNRHTTEAAAGALQNITAGDRRWAGVLSRLALEQERILNPLLDRVRTADHHQLRSLTGLIRNLSRN ARNKDEMSTKVVSHLIEKLPGSVGEKSPPAEVLVNIIAVLNNLVVASPIAARDLLYFDGLRKLIFIKKKR DSPDSEKSSRAASSLLANLWQYNKLHRDFRAKGYRKEDFLGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001303029 |
ORF Size | 2436 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001303029.2 |
RefSeq Size | 2843 bp |
RefSeq ORF | 2439 bp |
Locus ID | 11187 |
UniProt ID | Q9Y446 |
MW | 89.1 kDa |
Gene Summary | This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may act in cellular desmosome-dependent adhesion and signaling pathways. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] |
Documents
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