Plakophilin 3 (PKP3) (NM_001303029) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303029, the custom clone sequence may differ by one or more nucleotides ATGGAGTCCTGGACACCTCGGCCGTCAGCAGTGGCCTCTGGGATGAGCTGGGAGGCTGGCGGGATCCGGA CCACGAGCCGGCCTGAGGCCGGCGTGTGCTCCCTGGCGCTGCCCTCTGACCTGCAGCTGGACCGCCGGGG CGCCGAGGGGCCGGAGGCCGAGCGGCTGCGGGCAGCCCGCGTCCAGGAGCAGGTCCGCGCCCGCCTCTTG CAGCTGGGACAGCAGCCGCGGCACAACGGGGCCGCTGAGCCCGAGCCTGAGGCCGAGACTGCCAGAGGCA CATCCAGGGGGCAGTACCACACCCTGCAGGCTGGCTTCAGCTCTCGCTCTCAGGGCCTGAGTGGGGACAA GACCTCGGGCTTCCGGCCCATCGCCAAGCCGGCCTACAGCCCAGCCTCCTGGTCCTCCCGCTCCGCCGTG GATCTGAGCTGCAGTCGGAGGCTGAGTTCAGCCCACAACGGGGGCAGCGCCTTTGGGGCCGCTGGGTACG GGGGTGCCCAGCCCACCCCTCCCATGCCCACCAGGCCCGTGTCCTTCCATGAGCGCGGTGGGGTTGGGAG CCGGGCCGACTATGACACACTCTCCCTGCGCTCGCTGCGGCTGGGGCCCGGGGGCCTGGACGACCGCTAC AGCCTGGTGTCTGAGCAGCTGGAGCCCGCGGCCACCTCCACCTACAGGGCCTTTGCGTACGAGCGCCAGG CCAGCTCCAGCTCCAGCCGGGCAGGGGGGCTGGACTGGCCCGAGGCCACTGAGGTTTCCCCGAGCCGGAC CATCCGTGCCCCTGCCGTGCGGACCCTGCAGCGATTCCAGAGCAGCCACCGGAGCCGCGGGGTAGGCGGG GCAGTGCCGGGGGCCGTCCTGGAGCCAGTGGCTCGAGCGCCATCTGTGCGCAGCCTCAGCCTCAGCCTGG CTGACTCGGGCCACCTGCCGGACGTGCATGGGTTCAACAGCTACGGTAGCCACCGAACCCTGCAGAGACT CAGCAGCGGTTTTGATGACATTGACCTGCCCTCAGCAGTCAAGTACCTCATGGCTTCAGACCCCAACCTG CAGGTGCTGGGAGCGGCCTACATCCAGCACAAGTGCTACAGCGATGCAGCCGCCAAGAAGCAGGCCCGCA GCCTTCAGGCCGTGCCTAGGCTGGTGAAGCTCTTCAACCACGCCAACCAGGAAGTGCAGCGCCATGCCAC AGGTGCCATGCGCAACCTCATCTACGACAACGCTGACAACAAGCTGGCCCTGGTGGAGGAGAACGGGATC TTCGAGCTGCTGCGGACACTGCGGGAGCAGGATGATGAGCTTCGCAAAAATGTCACAGGGATCCTGTGGA ACCTTTCATCCAGCGACCACCTGAAGGACCGCCTGGCCAGAGACACGCTGGAGCAGCTCACAGACCTGGT GTTGAGCCCCCTGTCGGGGGCTGGGGGTCCCCCCCTCATCCAGCAGAACGCCTCGGAGGCAGAGATCTTC TACAACGCCACCGGCTTCCTCAGGAACCTCAGCTCAGCCTCTCAGGCCACTCGCCAGAAGATGCGGGAGT GCCACGGGCTGGTGGACGCCCTGGTCACCTCTATCAACCACGCCCTGGACGCGGGCAAATGCGAGGACAA GAGCGTGGAGAACGCGGTGTGCGTCCTGCGGAACCTGTCCTACCGCCTCTACGACGAGATGCCGCCGTCC GCGCTGCAGCGGCTGGAGGGTCGCGGCCGCAGGGACCTGGCGGGGGCGCCGCCGGGAGAGGTCGTGGGCT GCTTCACGCCGCAGAGCCGGCGGCTGCGCGAGCTGCCCCTCGCCGCCGATGCGCTCACCTTCGCGGAGGT GTCCAAGGACCCCAAGGGCCTCGAGTGGCTGTGGAGCCCCCAGATCGTGGGGCTGTACAACCGGCTGCTG CAGCGCTGCGAGCTCAACCGGCACACGACGGAGGCGGCCGCCGGGGCGCTGCAGAACATCACGGCAGGCG ACCGCAGGTGGGCGGGGGTGCTGAGCCGCCTGGCCCTGGAGCAGGAGCGTATTCTGAACCCCCTGCTAGA CCGTGTCAGGACCGCCGACCACCACCAGCTGCGCTCACTGACTGGCCTCATCCGAAACCTGTCTCGGAAC GCTAGGAACAAGGACGAGATGTCCACGAAGGTGGTGAGCCACCTGATCGAGAAGCTGCCGGGCAGCGTGG GTGAGAAGTCGCCCCCAGCCGAGGTGCTGGTCAACATCATAGCTGTGCTCAACAACCTGGTGGTGGCCAG CCCCATCGCTGCCCGAGACCTGCTGTATTTTGACGGACTCCGAAAGCTCATCTTCATCAAGAAGAAGCGG GACAGCCCCGACAGTGAGAAGTCCTCCCGGGCAGCATCCAGCCTCCTGGCCAACCTGTGGCAGTACAACA AGCTCCACCGTGACTTCCGGGCGAAGGGCTATCGGAAGGAGGACTTCCTGGGCCCATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001303029
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303029.1, NP_001289958.1
RefSeq Size	2843 bp
RefSeq ORF	2439 bp
Locus ID	11187
UniProt ID	Q9Y446
Gene Summary	This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may act in cellular desmosome-dependent adhesion and signaling pathways. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) encodes the longer isoform (PKP3b).

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