Noelin (OLFM1) (NM_001282611) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AMY; NOE1; NOELIN1; OlfA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238501 representing NM_001282611 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTGCCGCTGCTCAAGATCGGGGTCGTGCTGAGCACCATGGCCATGATCACTAACTGGATGTCCC AGACGCTGCCCTCGCTGGTGGGCCTCAACACCACCAAGCTCTCGGCGGCCGGCGGCGGGACGCTGGACCG CAGCACCGGCGTGCTGCCCACCAACCCTGAGGAGAGCTGGCAGGTGTACAGCTCTGCCCAGGACAGCGAG GGCAGGTGTATCTGCACAGTGGTCGCTCCACAGCAGACCATGTGTTCACGGGATGCCCGCACAAAACAGC TGAGGCAGCTACTGGAGAAGGTGCAGAACATGTCTCAATCCATAGAGGTCTTGGACAGGCGGACCCAGAG AGACTTGCAGTACGTGGAGAAGATGGAGAACCAAATGAAAGGACTGGAGTCCAAGTTCAAACAGGTGGAG GAGAGTCATAAGCAACACCTGGCCAGGCAGTTTAAGGCGATAAAAGCGAAAATGGATGAACTTAGGCCTT TGATACCTGTGTTGGAAGAGTACAAGGCCGATGCCAAATTGGTATTGCAGTTTAAAGAGGAGGTCCAGAA TCTGACGTCAGTGCTTAACGAGCTGCAAGAGGAAATTGGCGCCTATGACTACGATGAACTTCAGAGCAGA GTGTCCAATCTTGAAGAAAGGCTCCGTGCATGCATGCAAAAACTAGCTTGCGGGAAGTTGACGGGCATCA GTGACCCCGTGACTGTCAAGACCTCCGGCTCGAGGTTCGGATCCTGGATGACAGACCCTCTCGCCCCTGA AGGCGATAACCGGGTGTGGTACATGGACGGCTATCACAACAACCGCTTCGTACGTGAGTACAAGTCCATG GTTGACTTCATGAACACGGACAATTTCACCTCCCACCGTCTCCCCCACCCCTGGTCGGGCACGGGGCAGG TGGTCTACAACGGTTCTATCTACTTCAACAAGTTCCAGAGCCACATCATCATCAGGTTTGACCTGAAGAC AGAGACCATCCTCAAGACCCGCAGCCTGGACTATGCCGGTTACAACAACATGTACCACTACGCCTGGGGT GGCCACTCGGACATCGACCTCATGGTGGACGAGAGCGGGCTGTGGGCCGTGTACGCCACCAACCAGAACG CTGGCAACATCGTGGTCAGTAGGCTGGACCCCGTGTCCCTGCAGACCCTGCAGACCTGGAACACGAGCTA CCCCAAGCGCAGCGCCGGGGAGGCCTTCATCATCTGCGGCACGCTGTACGTCACCAACGGCTACTCAGGG GGTACCAAGGTCCACTATGCATACCAGACCAATGCCTCCACCTATGAATACATCGACATCCCATTCCAGA ACAAATACTCCCACATCTCCATGCTGGACTACAACCCCAAGGACCGGGCCCTGTATGCCTGGAACAACGG CCACCAGATCCTCTACAACGTGACCCTCTTCCACGTCATCCGCTCCGACGAGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238501 representing NM_001282611 Red=Cloning site Green=Tags(s) MSVPLLKIGVVLSTMAMITNWMSQTLPSLVGLNTTKLSAAGGGTLDRSTGVLPTNPEESWQVYSSAQDSE GRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQYVEKMENQMKGLESKFKQVE ESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQFKEEVQNLTSVLNELQEEIGAYDYDELQSR VSNLEERLRACMQKLACGKLTGISDPVTVKTSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSM VDFMNTDNFTSHRLPHPWSGTGQVVYNGSIYFNKFQSHIIIRFDLKTETILKTRSLDYAGYNNMYHYAWG GHSDIDLMVDESGLWAVYATNQNAGNIVVSRLDPVSLQTLQTWNTSYPKRSAGEAFIICGTLYVTNGYSG GTKVHYAYQTNASTYEYIDIPFQNKYSHISMLDYNPKDRALYAWNNGHQILYNVTLFHVIRSDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282611
ORF Size	1455 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282611.2
RefSeq Size	2791 bp
RefSeq ORF	1458 bp
Locus ID	10439
UniProt ID	Q99784
Protein Families	Druggable Genome
MW	55.8 kDa
Gene Summary	This gene product shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of the encoded protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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