Noelin (OLFM1) (NM_001282611) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AMY; NOE1; NOELIN1; OlfA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336395 representing NM_001282611. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGTGCCGCTGCTCAAGATCGGGGTCGTGCTGAGCACCATGGCCATGATCACTAACTGGATGTCC CAGACGCTGCCCTCGCTGGTGGGCCTCAACACCACCAAGCTCTCGGCGGCCGGCGGCGGGACGCTGGAC CGCAGCACCGGCGTGCTGCCCACCAACCCTGAGGAGAGCTGGCAGGTGTACAGCTCTGCCCAGGACAGC GAGGGCAGGTGTATCTGCACAGTGGTCGCTCCACAGCAGACCATGTGTTCACGGGATGCCCGCACAAAA CAGCTGAGGCAGCTACTGGAGAAGGTGCAGAACATGTCTCAATCCATAGAGGTCTTGGACAGGCGGACC CAGAGAGACTTGCAGTACGTGGAGAAGATGGAGAACCAAATGAAAGGACTGGAGTCCAAGTTCAAACAG GTGGAGGAGAGTCATAAGCAACACCTGGCCAGGCAGTTTAAGGCGATAAAAGCGAAAATGGATGAACTT AGGCCTTTGATACCTGTGTTGGAAGAGTACAAGGCCGATGCCAAATTGGTATTGCAGTTTAAAGAGGAG GTCCAGAATCTGACGTCAGTGCTTAACGAGCTGCAAGAGGAAATTGGCGCCTATGACTACGATGAACTT CAGAGCAGAGTGTCCAATCTTGAAGAAAGGCTCCGTGCATGCATGCAAAAACTAGCTTGCGGGAAGTTG ACGGGCATCAGTGACCCCGTGACTGTCAAGACCTCCGGCTCGAGGTTCGGATCCTGGATGACAGACCCT CTCGCCCCTGAAGGCGATAACCGGGTGTGGTACATGGACGGCTATCACAACAACCGCTTCGTACGTGAG TACAAGTCCATGGTTGACTTCATGAACACGGACAATTTCACCTCCCACCGTCTCCCCCACCCCTGGTCG GGCACGGGGCAGGTGGTCTACAACGGTTCTATCTACTTCAACAAGTTCCAGAGCCACATCATCATCAGG TTTGACCTGAAGACAGAGACCATCCTCAAGACCCGCAGCCTGGACTATGCCGGTTACAACAACATGTAC CACTACGCCTGGGGTGGCCACTCGGACATCGACCTCATGGTGGACGAGAGCGGGCTGTGGGCCGTGTAC GCCACCAACCAGAACGCTGGCAACATCGTGGTCAGTAGGCTGGACCCCGTGTCCCTGCAGACCCTGCAG ACCTGGAACACGAGCTACCCCAAGCGCAGCGCCGGGGAGGCCTTCATCATCTGCGGCACGCTGTACGTC ACCAACGGCTACTCAGGGGGTACCAAGGTCCACTATGCATACCAGACCAATGCCTCCACCTATGAATAC ATCGACATCCCATTCCAGAACAAATACTCCCACATCTCCATGCTGGACTACAACCCCAAGGACCGGGCC CTGTATGCCTGGAACAACGGCCACCAGATCCTCTACAACGTGACCCTCTTCCACGTCATCCGCTCCGAC GAGTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282611
Insert Size	1458 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282611.1
RefSeq Size	2791 bp
RefSeq ORF	1458 bp
Locus ID	10439
UniProt ID	Q99784
Protein Families	Druggable Genome
MW	55.3 kDa
Gene Summary	This gene product shares extensive sequence similarity with the rat neuronal olfactomedin-related ER localized protein. While the exact function of the encoded protein is not known, its abundant expression in brain suggests that it may have an essential role in nerve tissue. Several alternatively spliced transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) differs in the 5' UTR and 5' coding region which results in the use of an alternate start codon, compared to variant 1. The encoded isoform (4) has distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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