PMPCA (NM_001282946) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Alpha-MPP; CLA1; CPD3; INPP5E; MAS2; P-55; SCAR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238153 representing NM_001282946 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGAAATACCTTAGTGGAATTGCTCACTTTTTGGAAAAATTGGCATTTTCGTCTACTGCTCGATT TGACAGCAAAGATGAAATTCTGCTTACGTTGGAAAAGCATGGGGGTATCTGTGACTGCCAGACATCAAGA CACCACCATGTATGCTGTGTCTGCTGATAGCAAAGGCTTGGACACGGTGGTTGCCTTACTGGCTGATGTG GTTCTGCAGCCCCGGCTAACAGATGAAGAAGTCGAGATGACGCGGATGGCGGTCCAGTTTGAGCTGGAGG ACCTGAACCTGCGGCCTGACCCAGAGCCACTTCTCACCGAGATGATTCATGAAGCGGCTTACAGGGAGAA CACAGTTGGCCTCCACCGTTTCTGCCCCACAGAAAACGTAGCAAAGATCAACCGAGAGGTGCTGCATTCC TACCTGAGGAACTACTACACTCCCGACCGCATGGTGCTGGCCGGCGTGGGCGTGGAGCACGAGCATCTGG TGGACTGTGCCCGGAAGTACCTCCTGGGGGTCCAGCCGGCCTGGGGGAGCGCAGAGGCCGTGGATATTGA CAGATCTGTGGCCCAGTACACTGGGGGGATTGCCAAGCTAGAAAGAGACATGTCCAATGTCAGCCTGGGC CCGACCCCCATCCCCGAGCTCACGCACATCATGGTTGGACTGGAGAGCTGCTCCTTCCTGGAGGAGGACT TCATCCCCTTTGCAGTGTTGAACATGATGATGGGCGGAGGTGGCTCCTTCTCGGCTGGTGGGCCCGGCAA GGGCATGTTCTCCAGGCTCTACCTCAACGTGCTCAACAGGCACCACTGGATGTATAACGCGACCTCCTAC CACCACAGCTACGAGGACACTGGCCTCCTTTGCATCCATGCCAGCGCCGACCCAAGACAGGTTCGAGAAA TGGTAGAAATCATCACAAAGGAGTTTATTTTAATGGGCGGAACCGTGGACACGGTGGAGCTGGAACGAGC CAAGACGCAGCTGACATCAATGCTCATGATGAACCTGGAATCCAGGCCTGTGATCTTCGAGGATGTGGGG AGGCAGGTGCTGGCCACTCGCTCCAGAAAGCTGCCGCACGAGCTGTGCACGCTCATCCGCAACGTGAAGC CGGAAGATGTGAAGAGAGTCGCTTCTAAGATGCTCCGAGGGAAGCCGGCAGTGGCCGCCCTGGGTGACCT GACTGACCTGCCCACGTATGAGCACATCCAGACCGCCCTGTCGAGTAAGGACGGGCGCCTGCCCAGGACG TACCGGCTCTTCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238153 representing NM_001282946 Red=Cloning site Green=Tags(s) MKRNTLVELLTFWKNWHFRLLLDLTAKMKFCLRWKSMGVSVTARHQDTTMYAVSADSKGLDTVVALLADV VLQPRLTDEEVEMTRMAVQFELEDLNLRPDPEPLLTEMIHEAAYRENTVGLHRFCPTENVAKINREVLHS YLRNYYTPDRMVLAGVGVEHEHLVDCARKYLLGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNVSLG PTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSFSAGGPGKGMFSRLYLNVLNRHHWMYNATSY HHSYEDTGLLCIHASADPRQVREMVEIITKEFILMGGTVDTVELERAKTQLTSMLMMNLESRPVIFEDVG RQVLATRSRKLPHELCTLIRNVKPEDVKRVASKMLRGKPAVAALGDLTDLPTYEHIQTALSSKDGRLPRT YRLFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282946
ORF Size	1275 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282946.1, NP_001269875.1
RefSeq Size	2180 bp
RefSeq ORF	1278 bp
Locus ID	23203
UniProt ID	Q10713
Protein Families	Druggable Genome, Protease
MW	48.6 kDa
Gene Summary	The protein encoded by this gene is found in the mitochondrion, where it represents the alpha subunit of a proteolytic heterodimer. This heterodimer is responsible for cleaving the transit peptide from nuclear-encoded mitochondrial proteins. Defects in this gene are a cause of spinocerebellar ataxia, autosomal recessive 2. [provided by RefSeq, Mar 2016]

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