Myotilin (MYOT) (NM_001300911) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LGMD1; LGMD1A; MFM3; TTID; TTOD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237870 representing NM_001300911 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCCAACTATCAACAGTCCTCAGCTGGCCAACCTATAAATGCAAAGCCATCCCAAACTGCAAATG CTAAGCCCATACCAAGAACTCCTGATCATGAAATACAAGGATCAAAAGAAGCTTTGATTCAAGATTTGGA AAGAAAGCTGAAATGCAAGGACACCCTTCTTCATAATGGAAATCAACGTCTAACATATGAAGAGAAGATG GCTCGCAGATTGCTAGGACCACAGAATGCAGCTGCTGTGTTTCAAGCTCAGGATGACAGTGGTGCACAAG ACTCGCAGCAACACAACTCAGAACATGCGCGACTGCAAGTTCCTACATCACAAGTAAGAAGTAGATCAAC CTCAAGGGGAGATGTGAATGATCAGGATGCAATCCAGGAGAAATTTTACCCACCACGTTTCATTCAAGTG CCAGAGAACATGTCGATTGATGAAGGAAGATTCTGCAGAATGGACTTCAAAGTGAGTGGACTGCCAGCTC CTGATGTGTCATGGTATCTAAATGGAAGAACAGTTCAATCAGATGATTTGCACAAAATGATAGTGTCTGA GAAGGGTCTTCATTCACTCATCTTTGAAGTAGTCAGAGCTTCAGATGCAGGGGCTTATGCATGTGTTGCC AAGAATAGAGCAGGAGAAGCCACCTTCACTGTGCAGCTGGATGTCCTTGCAAAAGAACATAAAAGAGCAC CAATGTTTATCTACAAACCACAGAGCAAAAAAGTTTTAGAGGGAGATTCAGTGAAACTAGAATGCCAGAT CTCGGCTATACCTCCACCAAAGCTTTTCTGGAAAAGAAATAATGAAATGGTACAATTCAACACTGACCGA ATAAGCTTATATCAAGATAACACTGGAAGAGTTACTTTACTGATAAAAGATGTAAACAAGAAAGATGCTG GGTGGTATACTGTGTCAGCAGTTAATGAAGCTGGAGTGACTACATGTAACACAAGATTAGACGTTACGGC ACGTCCAAACCAAACTCTTCCAGCTCCTAAGCAGTTACGGGTTCGACCAACATTCAGCAAATATTTAGCA CTTAATGGGAAAGGTTTGAATGTAAAACAAGCTTTTAACCCAGAAGGAGAATTTCAGCGTTTGGCAGCTC AATCTGGACTCTATGAAAGTGAAGAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237870 representing NM_001300911 Red=Cloning site Green=Tags(s) MDSNYQQSSAGQPINAKPSQTANAKPIPRTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLTYEEKM ARRLLGPQNAAAVFQAQDDSGAQDSQQHNSEHARLQVPTSQVRSRSTSRGDVNDQDAIQEKFYPPRFIQV PENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVA KNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDR ISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLA LNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300911
ORF Size	1149 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300911.2
RefSeq Size	1939 bp
RefSeq ORF	1152 bp
Locus ID	9499
UniProt ID	Q9UBF9
MW	43.4 kDa
Gene Summary	This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.[provided by RefSeq, Oct 2008]

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