TRIB3 (NM_001301193) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C20orf97; NIPK; SINK; SKIP3; TRB3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237700 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC RCATGCGAGCCACCCCTCTGGCTGCTCCTGCGGGTTCCCTGTCCAGGAAGAAGCGGTTGGAGTTGGATGA CAACTTAGATACCGAGCGTCCCGTCCAGAAACGAGCTCGAAGTGGGCCCCAGCCCAGACTGCCCCCCTGC CTGTTGCCCCTGAGCCCACCTACTGCTCCAGATCGTGCAACTGCTGTGGCCACTGCCTCCCGTCTTGGGC CCTATGTCCTCCTGGAGCCCGAGGAGGGCGGGCGGGCCTACCAGGCCCTGCACTGCCCTACAGGCACTGA GTATACCTGCAAGGTGTACCCCGTCCAGGAAGCCCTGGCCGTGCTGGAGCCCTATGCGCGGCTGCCCCCG CACAAGCATGTGGCTCGGCCCACTGAGGTCCTGGCTGGTACCCAGCTCCTCTACGCCTTTTTCACTCGGA CCCATGGGGACATGCACAGCCTGGTGCGAAGCCGCCACCGTATCCCTGAGCCTGAGGCTGCCGTGCTCTT CCGCCAGATGGCCACCGCCCTGGCGCACTGTCACCAGCACGGTCTGGTCCTGCGTGATCTCAAGCTGTGT CGCTTTGTCTTCGCTGACCGTGAGAGGAAGAAGCTGGTGCTGGAGAACCTGGAGGACTCCTGCGTGCTGA CTGGGCCAGATGATTCCCTGTGGGACAAGCACGCGTGCCCAGCCTACGTGGGACCTGAGATACTCAGCTC ACGGGCCTCATACTCGGGCAAGGCAGCCGATGTCTGGAGCCTGGGCGTGGCGCTCTTCACCATGCTGGCC GGCCACTACCCCTTCCAGGACTCGGAGCCTGTCCTGCTCTTCGGCAAGATCCGCCGCGGGGCCTACGCCT TGCCTGCAGGCCTCTCGGCCCCTGCCCGCTGTCTGGTTCGCTGCCTCCTTCGTCGGGAGCCAGCTGAACG GCTCACAGCCACAGGCATCCTCCTGCACCCCTGGCTGCGACAGGACCCGATGCCCTTAGCCCCAACCCGA TCCCATCTCTGGGAGGCTGCCCAGGTGGTCCCTGATGGACTGGGGCTGGACGAAGCCAGGGAAGAGGAGG GAGACAGAGAAGTGGTTCTGTATGGC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237700 protein sequence Red=Cloning site Green=Tags(s) XCEPPLWLLLRVPCPGRSGWSWMTT*IPSVPSRNELEVGPSPDCPPACCP*AHLLLQIVQLLWPLPPVLG PMSSWSPRRAGGPTRPCTALQALSIPARCTPSRKPWPCWSPMRGCPRTSMWLGPLRSWLVPSSSTPFSLG PMGTCTAWCEAATVSLSLRLPCSSARWPPPWRTVTSTVWSCVISSCVALSSLTVRGRSWCWRTWRTPAC* LGQMIPCGTSTRAQPTWDLRYSAHGPHTRARQPMSGAWAWRSSPCWPATTPSRTRSLSCSSARSAAGPTP CLQASRPLPAVWFAASFVGSQLNGSQPQASSCTPGCDRTRCP*PQPDPISGRLPRWSLMDWGWTKPGKRR ETEKWFCM TRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301193
ORF Size	1074 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301193.1, NP_001288122.1
RefSeq Size	2160 bp
RefSeq ORF	1077 bp
Locus ID	57761
UniProt ID	Q96RU7
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
MW	39.6 kDa
Gene Summary	The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]

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