WIPI2 (NM_001278299) Human Tagged ORF Clone
CAT#: RC237362
- TrueORF®
WIPI2 (myc-DDK-tagged) - Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 6
ORF Plasmid: tGFP
"NM_001278299" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237362 representing NM_001278299
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGCTGCATACGATCAGGGAGACGCCTCCAAACCCTGCAGGCCTGTGTGCGCTGTCAATCAACA ACGACAACTGCTACTTGGCGTACCCAGGGAGCGCGACCATCGGAGAGGTGCAGGTCTTCGATACCATTAA TTTGAGAGCTGCAAACATGATTCCGGCTCACGACAGTCCTTTAGCGGCACTGGCCTTTGACGCAAGTGGA ACTAAACTTGCCACGGCTTCGGAGAAGGGGACCGTGATTAGGGTATTTTCCATTCCAGAAGGACAAAAAC TCTTTGAGTTTCGGAGAGGAGTAAAGAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAGCATGGACGGCAT GTTCCTCTCCGCCTCCAGCAACACTGAGACCGTGCACATCTTCAAACTCGAGACTGTGAAAGAAAAACCC CCAGAGGAGCCCACCACCTGGACCGGGTACTTCGGGAAAGTGCTCATGGCCTCCACCAGCTACCTGCCTT CCCAAGTGACAGAAATGTTCAACCAGGGCAGAGCCTTCGCCACGGTCCGCCTGCCATTCTGCGGCCACAA AAACATCTGCTCGCTAGCCACAATTCAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCCGACGGGTACCTG TACATGTACAACCTGGACCCCCAGGAGGGCGGCGAGTGTGCCCTGATGAAGCAGCACCGGCTGGACGGCA GTCTGGAAACGACCAATGAGATCTTGGACTCTGCCTCTCACGACTGCCCCTTAGTCACTCAGACATACGG CGCAGCTGCAGGAAAAGGTACTTACGTGCCTTCATCCCCAACGAGACTTGCCTACACAGACGACCTGGGT GCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACAGCGAGCACCCGC CCATGATTCTTCGGACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237362 representing NM_001278299
Red=Cloning site Green=Tags(s) MKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASG TKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKP PEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYL YMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYTDDLG AVGGACLEDEASALRLDEDSEHPPMILRTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278299 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001278299.2 |
RefSeq Size | 4663 bp |
RefSeq ORF | 933 bp |
Locus ID | 26100 |
UniProt ID | Q9Y4P8 |
MW | 34 kDa |
Gene Summary | WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008] |
Documents
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FAQs |
SDS |